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Information on EC 2.4.1.10 - levansucrase
for references in articles please use BRENDA:EC2.4.1.10
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IUBMB Comments
Some other sugars can act as D-fructosyl acceptors.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
- 2.4.1.10
-
fructosylation
- fructooligosaccharides
-
transfructosylation
- mobilis
-
zymomonas
- exopolysaccharide
- erwinia
- raffinose
-
prebiotics
- inulin
-
levan-type
- diazotrophicus
- fructosyltransferases
- leuconostoc
- reuteri
- inulosucrase
-
gluconacetobacter
- mesenteroides
- dextransucrase
-
sucrose:sucrose
- synthesis
-
gluconobacter
- 6-kestose
- aquatilis
-
rahnella
-
exocellular
- invertases
-
hpaec-pad
-
sourdough
-
amylovoran
-
antitermination
- oxydans
- food industry
- agriculture
Synonyms
levansucrase, 6-sft, sucrose:fructan 6-fructosyltransferase, fructansucrase, lsc-3, t2-ls, t1-ls, sucrose 6-fructosyltransferase, more
top
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
6-SFT
beta-2,6-fructan:D-glucose 1-fructosyltransferase
-
-
-
-
beta-2,6-fructosyltransferase
-
-
-
-
fructansucrase
fructosyltransferase, sucrose 6-
-
-
-
-
Lev
levanase-sucrase
levansucrase
LevB1SacB
LevJ
LEVS
Lsc
Lsc-3
Lsc2
Lsc3
LscrA
LsdA
LvnS
m1ft
M1FT protein
SacB
sucrose 6-fructosyltransferase
-
-
-
-
sucrose: 2, 6-beta-D-fructan 6-beta-Dfructosyltransferase
sucrose:2,6-beta-D-fructan:6-beta-D-fructosyltransferase
sucrose:fructan 6-fructosyltransferase
T1-LS
T2-LS
sucrose: 2, 6-beta-D-fructan 6-beta-Dfructosyltransferase
-
sucrose:2,6-beta-D-fructan:6-beta-D-fructosyltransferase
-
sucrose:2,6-beta-D-fructan:6-beta-D-fructosyltransferase
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
mechanism
-
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
ping-pong mechanism
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sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
ping-pong mechanism
-
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
some other sugars can act as D-fructosyl acceptors
-
-
-
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
formation of a transient fructosyl-enzyme intermediate, kinetics of the hydrolase, exchange and transfructosylation reaction
-
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
N-terminal catalytic domain, C-terminal glucan-binding domain with glycine-rich repeats
-
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
N-terminal catalytic domain, C-terminal glucan-binding domain with glycine-rich repeats
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
reaction kinetics are dependent on sucrose concentration
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sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
Glu278, located in DXXER conserved motif, and Asp194, located in conserved RDP motif, and His296 are involved in catalysis
-
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
Asp309 is involved in catalytic ping-pong mechanism, localization in conserved RDP-motif
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
N-terminal catalytic domain, C-terminal glucan-binding domain with glycine-rich repeats
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
hexosyl group transfer
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-
-
-
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SYSTEMATIC NAME
IUBMB Comments
sucrose:[6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside 6-beta-D-fructosyltransferase
Some other sugars can act as D-fructosyl acceptors.
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CAS REGISTRY NUMBER
COMMENTARY
9030-17-5
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1-kestotriose + sucrose
1,6-kestotetraose + ?
-
Substrates: 1-kestotriose is the preferred substrate
Products: -
Products: -
?
2 sucrose
D-glucose + beta-D-fructofuranosyl-(2,6)-beta-D-fructofuranosyl-(2,1)-alpha-D-glucopyranoside
alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside
?
alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside
?
alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside
?
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n
D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-beta-D-fructosyl]n
D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-beta-D-fructosyl]n+1
sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside
D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside
sucrose + cellobiose
beta-D-glucopyranosyl-(1,4)-alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside + D-glucose
sucrose + cellobiose
D-glucose + beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
sucrose + D-xylose
beta-D-fructofuranosyl-beta-D-xylopyranoside + D-glucose
Substrates: -
Products: -
Products: -
?
sucrose + D-xylose
D-glucose + beta-D-fructofuranosyl-(2,1)-alpha-D-xylopyranoside
-
Substrates: -
Products: NMR product analysis
Products: NMR product analysis
?
sucrose + H2O
glucose + fructose + levan + sucrose + fructooligosaccharides
-
Substrates: -
Products: -
Products: -
?
sucrose + isomaltose
isomaltosyl-fructose + D-glucose
Substrates: -
Products: -
Products: -
?
sucrose + L-arabinose
6-kestotriose + ?
Substrates: -
Products: -
Products: -
?
sucrose + L-arabinose
6-kestotriose + fructosylated L-arabinose
Substrates: -
Products: -
Products: -
?
sucrose + L-galactose
beta-D-fructofuranosyl-beta-L-galactopyranoside + D-glucose
Substrates: -
Products: -
Products: -
?
sucrose + L-glucose
beta-D-fructofuranosyl-beta-L-glucopyranoside + D-glucose
Substrates: -
Products: -
Products: -
?
sucrose + L-xylose
beta-D-fructofuranosyl-beta-L-xylopyranoside + D-glucose
Substrates: -
Products: -
Products: -
?
sucrose + lactose
beta-D-galactopyranosyl-(1,4)-alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside + D-glucose
Substrates: -
Products: -
Products: -
?
sucrose + lactose
D-glucose + beta-D-fructofuranosyl-beta-D-galactopyranosyl-(1-4)-beta-D-glucopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + lactose
D-glucose + beta-D-galactopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
sucrose + lactose
D-glucose + beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
sucrose + lactose
D-glucose + O-beta-D-galactopyranosyl-(1,4)-O-beta-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside
sucrose + lactose
fructosylated lactose + ?
Substrates: -
Products: -
Products: -
?
sucrose + lactose
lactosucrose + D-glucose
Substrates: the production condition is optimized as pH at 6.0, temperature at 35°C, 5 units/ml enzyme, 180 g/l sucrose, and 180 g/l lactose
Products: -
Products: -
?
sucrose + levan
?
Substrates: mutant enzymes H243L and S164A synthesize either high or low levan molecular weight
Products: -
Products: -
?
sucrose + maltose
D-glucose + alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
sucrose + polygalacturonic acid
?
Substrates: -
Products: -
Products: -
?
sucrose + raffinose
Fru(beta-2,1)Gal + fructosylated lactose + fructosylated cellobiose + 1-kestotriose + 6-kestotriose + ?
Substrates: -
Products: -
Products: -
?
sucrose + raffinose
melibiose + D-fructose + levan
Substrates: -
Products: -
Products: -
?
sucrose + stachyose
manninotriose + D-fructose
Substrates: -
Products: -
Products: -
?
sucrose + sucrose
D-glucose + (2,6-beta-D-fructosyl)3
Q60114
Substrates: dimer form
Products: degree of polymerisation 3
Products: degree of polymerisation 3
?
sucrose + sucrose
D-glucose + 6-kestose + 6-nystose + 6,6,6-kestopentaose + levanbiose + levantriose + ?
sucrose + sucrose
D-glucose + levan + fructooligosaccharides
Substrates: -
Products: -
Products: -
?
sucrose + sucrose
D-glucose + low-molecular weight levan + short-chain fructooligosaccharides (DP 3-6)
sucrose + sucrose
D-glucose + neokestose
-
Substrates: -
Products: identified by one-dimensional and correlation spectroscopy (i.e. COSY, TOCSY, HMBC, DEPT and HSQC)
Products: identified by one-dimensional and correlation spectroscopy (i.e. COSY, TOCSY, HMBC, DEPT and HSQC)
?
sucrose + sucrose
D-glucose + nystose
-
Substrates: -
Products: identified by HPAEC
Products: identified by HPAEC
?
sucrose + sucrose
kestose + D-glucose
-
Substrates: high concentration of sucrose
Products: -
Products: -
?
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside
D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
Q60114
Substrates: pH lower than 7.0, fibril form
Products: -
Products: -
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
surgarcane molasse
D-glucose + fructose + kestose + nystose + difructose + levan
Substrates: -
Products: -
Products: -
?
sweet sorghum juice
D-glucose + fructose + kestose + nystose + difructose + levan
Substrates: -
Products: -
Products: -
?
table sugar
D-glucose + fructose + kestose + nystose + difructose + levan
Substrates: -
Products: -
Products: -
?
2 sucrose
6-kestose + D-glucose
-
Substrates: -
Products: -
Products: -
?
2 sucrose
6-kestose + D-glucose
Substrates: -
Products: -
Products: -
?
2 sucrose
6-kestose + D-glucose
Substrates: -
Products: -
Products: -
?
2 sucrose
6-kestose + D-glucose
Substrates: -
Products: -
Products: -
?
2 sucrose
6-kestose + D-glucose
Substrates: -
Products: -
Products: -
?
2 sucrose
D-glucose + beta-D-fructofuranosyl-(2,6)-beta-D-fructofuranosyl-(2,1)-alpha-D-glucopyranoside
-
Substrates: -
Products: -
Products: -
?
2 sucrose
D-glucose + beta-D-fructofuranosyl-(2,6)-beta-D-fructofuranosyl-(2,1)-alpha-D-glucopyranoside
-
Substrates: -
Products: -
Products: -
?
alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside
?
-
Substrates: besides the major product, levan, the enzyme produces two galactosyloligofructosides with D-Gal-Fru
Products: -
Products: -
?
alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside
?
-
Substrates: besides the major product, levan, the enzyme produces two galactosyloligofructosides with D-Gal-Fru
Products: -
Products: -
?
alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside
?
-
Substrates: only one mannosyloligofructoside is formed
Products: -
Products: -
?
alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside
?
-
Substrates: only one mannosyloligofructoside is formed
Products: -
Products: -
?
alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside
?
-
Substrates: besides the major product, levan, the enzyme produces at least three different xylosyloligofructosides with D-Xyl-Fru
Products: -
Products: -
?
alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside
?
-
Substrates: besides the major product, levan, the enzyme produces at least three different xylosyloligofructosides with D-Xyl-Fru
Products: -
Products: -
?
jaggary
D-glucose + fructose + kestose + nystose + difructose + levan
Substrates: -
Products: -
Products: -
?
jaggary
D-glucose + fructose + kestose + nystose + difructose + levan
Leuconostoc mesenteroides MTCC10508
Substrates: -
Products: -
Products: -
?
raffinose + (2,6-beta-D-fructosyl)n
galactose + (2,6-beta-D-fructosyl)n+1
-
Substrates: -
Products: the levan synthesized on raffinose contains one mol of galactosylglucose per mol as one of the 2 terminal glycosyl moieties
Products: the levan synthesized on raffinose contains one mol of galactosylglucose per mol as one of the 2 terminal glycosyl moieties
?
raffinose + (2,6-beta-D-fructosyl)n
galactose + (2,6-beta-D-fructosyl)n+1
-
Substrates: in 0.38 M phosphate buffer
Products: -
Products: -
?
raffinose + (2,6-beta-D-fructosyl)n
galactose + (2,6-beta-D-fructosyl)n+1
-
Substrates: -
Products: -
Products: -
?
sucrose
levan + fructooligosaccharides
Substrates: the reaction occurs only in the presence of high salt concentrations (more than 1.5 M NaCl)
Products: -
Products: -
?
sucrose
levan + fructooligosaccharides
Substrates: the reaction occurs only in the presence of high salt concentrations (more than 1.5 M NaCl)
Products: -
Products: -
?
sucrose
levan + kestose
-
Substrates: at concentrations below 85 mM (at 37°C). Above 85 mM sucrose, the transglycosylation activity of Lev gradually becomes significant. Transglycosylation never exceeds 50% of total enzyme activity
Products: -
Products: -
?
sucrose
levan + kestose
-
Substrates: at concentrations below 85 mM (at 37°C). Above 85 mM sucrose, the transglycosylation activity of Lev gradually becomes significant. Transglycosylation never exceeds 50% of total enzyme activity
Products: -
Products: -
?
sucrose + (2,6-beta-D-fructosyl)n
alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
-
Substrates: -
Products: -
Products: -
?
sucrose + (2,6-beta-D-fructosyl)n
alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
-
Substrates: capacity to produce extracellular homopolysaccharides of fructose, modulation of the composition of the biofilms that form on teeth
Products: -
Products: -
?
sucrose + (2,6-beta-D-fructosyl)n
alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
-
Substrates: -
Products: -
Products: -
?
sucrose + (2,6-beta-D-fructosyl)n
alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
-
Substrates: capacity to produce extracellular homopolysaccharides of fructose, modulation of the composition of the biofilms that form on teeth
Products: -
Products: -
?
sucrose + (2,6-beta-D-fructosyl)n
D-glucose + (2,6-beta-D-fructosyl)n+1
Substrates: -
Products: -
Products: -
?
sucrose + (2,6-beta-D-fructosyl)n
D-glucose + (2,6-beta-D-fructosyl)n+1
-
Substrates: reduction of glucose by yeasts (Candida cacaoi DSM 2226), introduced into a dialysing membrane, situated in the reaction medium, and the presence of Mn2+ results in an increase of levan synthesis efficiency to 64%
Products: -
Products: -
?
sucrose + (2,6-beta-D-fructosyl)n
D-glucose + (2,6-beta-D-fructosyl)n+1
-
Substrates: -
Products: -
Products: -
?
sucrose + (2,6-beta-D-fructosyl)n
D-glucose + (2,6-beta-D-fructosyl)n+1
-
Substrates: polyfructan produced by the levansucrase from Bacillus megaterium has a molecular mass of 2711 kDa and consisted mainly of beta(2,6) linkages. Besides the polyfructan formation, the wild-type levansucrase of Bacillus megaterium also synthesizes five different detectable oligosaccharides. Three products are identified: 1-kestose (isokestose), 6-kestose and nystose which are known acceptors for the transfer of fructosyl units
Products: -
Products: -
?
sucrose + (2,6-beta-D-fructosyl)n
D-glucose + (2,6-beta-D-fructosyl)n+1
-
Substrates: polyfructan produced by the levansucrase from Bacillus megaterium has a molecular mass of 2711 kDa and consisted mainly of beta(2,6) linkages. Besides the polyfructan formation, the wild-type levansucrase of Bacillus megaterium also synthesizes five different detectable oligosaccharides. Three products are identified: 1-kestose (isokestose), 6-kestose and nystose which are known acceptors for the transfer of fructosyl units
Products: -
Products: -
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
Substrates: reverse reaction is probably catalyzed by a different levanase
Products: -
Products: -
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
Substrates: enzyme is probably involved in initiation and progression of dental caries and periodontal diseases
Products: -
Products: -
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
Substrates: reverse reaction is probably catalyzed by a different levanase
Products: -
Products: -
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
Substrates: enzyme is probably involved in initiation and progression of dental caries and periodontal diseases
Products: -
Products: -
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
Substrates: -
Products: high molecular weight branched levan
Products: high molecular weight branched levan
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
Substrates: -
Products: product forms a complex with the enzyme
Products: product forms a complex with the enzyme
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
Aerobacter levanicum
-
Substrates: -
Products: product forms a complex with the enzyme
Products: product forms a complex with the enzyme
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
Aerobacter levanicum
-
Substrates: levan biosynthesis
Products: -
Products: -
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
Substrates: -
Products: -
Products: -
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
Substrates: activity is affected by sacU mutation
Products: -
Products: -
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
Substrates: -
Products: -
Products: -
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
Substrates: -
Products: -
Products: -
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
Substrates: -
Products: -
Products: -
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1