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sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
mechanism
-
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
ping-pong mechanism
-
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
ping-pong mechanism
-
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
some other sugars can act as D-fructosyl acceptors
-
-
-
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
formation of a transient fructosyl-enzyme intermediate, kinetics of the hydrolase, exchange and transfructosylation reaction
-
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
N-terminal catalytic domain, C-terminal glucan-binding domain with glycine-rich repeats
-
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
N-terminal catalytic domain, C-terminal glucan-binding domain with glycine-rich repeats
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
reaction kinetics are dependent on sucrose concentration
-
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
Glu278, located in DXXER conserved motif, and Asp194, located in conserved RDP motif, and His296 are involved in catalysis
-
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
Asp309 is involved in catalytic ping-pong mechanism, localization in conserved RDP-motif
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
N-terminal catalytic domain, C-terminal glucan-binding domain with glycine-rich repeats
-
-
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1-kestotriose + sucrose
1,6-kestotetraose + ?
-
1-kestotriose is the preferred substrate
-
-
?
2 sucrose
6-kestose + D-glucose
2 sucrose
D-glucose + beta-D-fructofuranosyl-(2,6)-beta-D-fructofuranosyl-(2,1)-alpha-D-glucopyranoside
6G-kestotriose + sucrose
6-kestotriose + ?
-
-
-
-
?
alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside
?
alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside
?
alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside
?
D-raffinose
D-fructose + melibiose
-
-
-
?
jaggary
D-glucose + fructose + kestose + nystose + difructose + levan
raffinose + (2,6-beta-D-fructosyl)n
galactose + (2,6-beta-D-fructosyl)n+1
stachyose + (2,6-beta-D-fructosyl)n
?
-
-
-
-
?
sucrose
levan + fructooligosaccharides
sucrose + (2,6-beta-D-fructosyl)n
alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
sucrose + (2,6-beta-D-fructosyl)n
D-glucose + (2,6-beta-D-fructosyl)n+1
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
sucrose + ?
polyfructan + ?
sucrose + acarbose
D-glucose + 1'-beta-D-fructofuranosyl alpha-acarbose
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n
D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-alpha-D-fructosyl]n+1
sucrose + alpha-D-glucosyl-(1->2)-[(2->6)-beta-D-fructosyl]n
D-glucose + alpha-D-glucosyl-(1->2)-[(2->6)-beta-D-fructosyl]n+1
sucrose + arabinose
?
-
-
-
?
sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside
D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside
sucrose + cellobiose
?
-
-
-
-
?
sucrose + cellobiose
beta-D-glucopyranosyl-(1,4)-alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside + D-glucose
sucrose + cellobiose
D-glucose + beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
sucrose + cellobiose
fructosylated cellobiose + ?
sucrose + D-arabinose
?
-
-
-
-
?
sucrose + D-fucose
beta-D-fructofuranosyl-alpha-D-fucopyranoside + D-glucose
sucrose + D-galactose
?
-
-
-
-
?
sucrose + D-galactose
beta-D-fructofuranosyl-alpha-D-galactopyranoside + D-glucose
sucrose + D-glucose
glucose + sucrose
sucrose + D-xylose
beta-D-fructofuranosyl-alpha-D-xylopyranoside + D-glucose
sucrose + D-xylose
beta-D-fructofuranosyl-beta-D-xylopyranoside + D-glucose
-
-
-
?
sucrose + D-xylose
D-glucose + alpha-D-xylopyranosyl-(1->2)-beta-D-fructofuranoside
sucrose + D-xylose
D-glucose + beta-D-fructofuranosyl-(2,1)-alpha-D-xylopyranoside
-
-
NMR product analysis
-
?
sucrose + fructose
?
-
-
-
?
sucrose + galactose
Fru(beta-2,6)Gal + ?
sucrose + glucose
1-kestotriose + 6-kestotriose + 1-kestotetraose + ?
sucrose + glucose
?
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
sucrose + H2O
glucose + fructose + levan + sucrose + fructooligosaccharides
-
-
-
-
?
sucrose + hydroquinone
D-glucose + 4-hydroxyphenyl-beta-D-fructofuranoside
sucrose + inositol
?
-
low activity
-
-
?
sucrose + inulin
?
-
-
-
-
?
sucrose + isomaltose
D-glucose + theanderose
sucrose + isomaltose
isomaltosyl-fructose + D-glucose
-
-
-
?
sucrose + L-arabinose
6-kestotriose + ?
-
-
-
?
sucrose + L-arabinose
6-kestotriose + fructosylated L-arabinose
-
-
-
?
sucrose + L-arabinose
?
-
-
-
-
?
sucrose + L-galactose
beta-D-fructofuranosyl-beta-L-galactopyranoside + D-glucose
-
-
-
?
sucrose + L-glucose
beta-D-fructofuranosyl-beta-L-glucopyranoside + D-glucose
-
-
-
?
sucrose + L-xylose
beta-D-fructofuranosyl-beta-L-xylopyranoside + D-glucose
-
-
-
?
sucrose + lactose
beta-D-galactopyranosyl-(1,4)-alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside + D-glucose
-
-
-
?
sucrose + lactose
D-glucose + beta-D-fructofuranosyl-beta-D-galactopyranosyl-(1-4)-beta-D-glucopyranoside
-
-
-
-
?
sucrose + lactose
D-glucose + beta-D-galactopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
sucrose + lactose
D-glucose + beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
sucrose + lactose
D-glucose + lactosylfructoside
sucrose + lactose
D-glucose + O-beta-D-galactopyranosyl-(1,4)-O-beta-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside
sucrose + lactose
fructosylated lactose + ?
-
-
-
?
sucrose + lactose
lactosucrose
sucrose + lactose
lactosucrose + D-glucose
the production condition is optimized as pH at 6.0, temperature at 35°C, 5 units/ml enzyme, 180 g/l sucrose, and 180 g/l lactose
-
-
?
sucrose + levan
?
mutant enzymes H243L and S164A synthesize either high or low levan molecular weight
-
-
?
sucrose + maltose
D-glucose + alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside
sucrose + maltose
erlose + D-glucose
-
-
-
?
sucrose + maltotriose
?
-
-
-
-
?
sucrose + mannose
?
-
-
-
?
sucrose + melibiose
?
-
-
-
-
?
sucrose + melibiose
D-glucose + raffinose
sucrose + melibiose
raffinose + D-glucose
sucrose + methanol
glucose + methyl-beta-D-fructoside
sucrose + polygalacturonic acid
?
-
-
-
?
sucrose + raffinose
Fru(beta-2,1)Gal + fructosylated lactose + fructosylated cellobiose + 1-kestotriose + 6-kestotriose + ?
-
-
-
?
sucrose + raffinose
melibiose + ?
-
-
-
-
?
sucrose + raffinose
melibiose + D-fructose + levan
-
-
-
?
sucrose + stachyose
manninotriose + D-fructose
-
-
-
?
sucrose + sucrose
1-kestose + 6-kestose + neo-kestose + ?
sucrose + sucrose
6-kestose + levanbiose + blastose + 1-kestose + ?
sucrose + sucrose
D-glucose + (2,6-beta-D-fructosyl)3
Q60114
dimer form
degree of polymerisation 3
-
?
sucrose + sucrose
D-glucose + 1-kestose
sucrose + sucrose
D-glucose + 1-kestose + nystose + ?
sucrose + sucrose
D-glucose + 6-kestose
sucrose + sucrose
D-glucose + 6-kestose + 6-nystose + 6,6,6-kestopentaose + levanbiose + levantriose + ?
sucrose + sucrose
D-glucose + levan
sucrose + sucrose
D-glucose + levan + fructooligosaccharides
-
-
-
?
sucrose + sucrose
D-glucose + levan type-fructan
sucrose + sucrose
D-glucose + low-molecular weight levan + short-chain fructooligosaccharides (DP 3-6)
sucrose + sucrose
D-glucose + neokestose
-
-
identified by one-dimensional and correlation spectroscopy (i.e. COSY, TOCSY, HMBC, DEPT and HSQC)
-
?
sucrose + sucrose
D-glucose + nystose
-
-
identified by HPAEC
-
?
sucrose + sucrose
kestose + D-glucose
-
high concentration of sucrose
-
-
?
sucrose + triisopropylbenzenesulfonyllevan
?
-
-
-
-
?
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside
D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
Q60114
pH lower than 7.0, fibril form
-
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
surgarcane molasse
D-glucose + fructose + kestose + nystose + difructose + levan
-
-
-
?
sweet sorghum juice
D-glucose + fructose + kestose + nystose + difructose + levan
-
-
-
?
table sugar
D-glucose + fructose + kestose + nystose + difructose + levan
-
-
-
?
additional information
?
-
2 sucrose
6-kestose + D-glucose
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
?
2 sucrose
6-kestose + D-glucose
-
-
-
-
?
2 sucrose
D-glucose + beta-D-fructofuranosyl-(2,6)-beta-D-fructofuranosyl-(2,1)-alpha-D-glucopyranoside
-
-
-
-
?
2 sucrose
D-glucose + beta-D-fructofuranosyl-(2,6)-beta-D-fructofuranosyl-(2,1)-alpha-D-glucopyranoside
-
-
-
-
?
alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside
?
-
besides the major product, levan, the enzyme produces two galactosyloligofructosides with D-Gal-Fru
-
-
?
alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside
?
-
besides the major product, levan, the enzyme produces two galactosyloligofructosides with D-Gal-Fru
-
-
?
alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside
?
-
only one mannosyloligofructoside is formed
-
-
?
alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside
?
-
only one mannosyloligofructoside is formed
-
-
?
alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside
?
-
besides the major product, levan, the enzyme produces at least three different xylosyloligofructosides with D-Xyl-Fru
-
-
?
alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside
?
-
besides the major product, levan, the enzyme produces at least three different xylosyloligofructosides with D-Xyl-Fru
-
-
?
inulin + H2O
?
-
-
-
-
?
jaggary
D-glucose + fructose + kestose + nystose + difructose + levan
-
-
-
?
jaggary
D-glucose + fructose + kestose + nystose + difructose + levan
-
-
-
?
raffinose + (2,6-beta-D-fructosyl)n
galactose + (2,6-beta-D-fructosyl)n+1
-
-
the levan synthesized on raffinose contains one mol of galactosylglucose per mol as one of the 2 terminal glycosyl moieties
?
raffinose + (2,6-beta-D-fructosyl)n
galactose + (2,6-beta-D-fructosyl)n+1
-
in 0.38 M phosphate buffer
-
-
?
raffinose + (2,6-beta-D-fructosyl)n
galactose + (2,6-beta-D-fructosyl)n+1
-
-
-
-
?
sucrose
levan + fructooligosaccharides
the reaction occurs only in the presence of high salt concentrations (more than 1.5 M NaCl)
-
-
?
sucrose
levan + fructooligosaccharides
the reaction occurs only in the presence of high salt concentrations (more than 1.5 M NaCl)
-
-
?
sucrose
levan + kestose
-
at concentrations below 85 mM (at 37°C). Above 85 mM sucrose, the transglycosylation activity of Lev gradually becomes significant. Transglycosylation never exceeds 50% of total enzyme activity
-
-
?
sucrose
levan + kestose
-
at concentrations below 85 mM (at 37°C). Above 85 mM sucrose, the transglycosylation activity of Lev gradually becomes significant. Transglycosylation never exceeds 50% of total enzyme activity
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
-
capacity to produce extracellular homopolysaccharides of fructose, modulation of the composition of the biofilms that form on teeth
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
alpha-D-glucose + (2,6-beta-D-fructosyl)n+1
-
capacity to produce extracellular homopolysaccharides of fructose, modulation of the composition of the biofilms that form on teeth
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
D-glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
D-glucose + (2,6-beta-D-fructosyl)n+1
-
reduction of glucose by yeasts (Candida cacaoi DSM 2226), introduced into a dialysing membrane, situated in the reaction medium, and the presence of Mn2+ results in an increase of levan synthesis efficiency to 64%
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
D-glucose + (2,6-beta-D-fructosyl)n+1
-
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
D-glucose + (2,6-beta-D-fructosyl)n+1
-
polyfructan produced by the levansucrase from Bacillus megaterium has a molecular mass of 2711 kDa and consisted mainly of beta(2,6) linkages. Besides the polyfructan formation, the wild-type levansucrase of Bacillus megaterium also synthesizes five different detectable oligosaccharides. Three products are identified: 1-kestose (isokestose), 6-kestose and nystose which are known acceptors for the transfer of fructosyl units
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
D-glucose + (2,6-beta-D-fructosyl)n+1
-
polyfructan produced by the levansucrase from Bacillus megaterium has a molecular mass of 2711 kDa and consisted mainly of beta(2,6) linkages. Besides the polyfructan formation, the wild-type levansucrase of Bacillus megaterium also synthesizes five different detectable oligosaccharides. Three products are identified: 1-kestose (isokestose), 6-kestose and nystose which are known acceptors for the transfer of fructosyl units
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
reverse reaction is probably catalyzed by a different levanase
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
enzyme is probably involved in initiation and progression of dental caries and periodontal diseases
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fr
2.4.1.10 levansucrase
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