General Stability | Organism |
---|---|
DTT and glycerol stabilize | Colwellia maris |
DTT stabilizes | Colwellia maris |
salt does not stabilize | Colwellia maris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | - |
Colwellia maris | |
NaCl | - |
Colwellia maris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0198 | - |
glyoxylate | - |
Colwellia maris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | about 35% of the activation with Mg2+ | Colwellia maris | |
Mg2+ | required | Colwellia maris | |
Mn2+ | 19% of the activation with Mg2+ | Colwellia maris | |
Ni2+ | about 98% of the activation with Mg2+ | Colwellia maris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
gel filtration | Colwellia maris |
763000 | - |
2 * 763000, SDS-PAGE | Colwellia maris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + acetyl-CoA + H2O | Colwellia maris | enzyme specifically involved in glyoxalate cycle metabolism | (S)-malate + CoA | - |
? | |
glyoxylate + acetyl-CoA + H2O | Colwellia maris | enzyme essential for acetate use in the bacterial cells | (S)-malate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Colwellia maris | - |
formerly Vibrio sp. strain ABE-1 | - |
Purification (Comment) | Organism |
---|---|
- |
Colwellia maris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:acetate-grown cell | - |
Colwellia maris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
69.6 | - |
- |
Colwellia maris |
Storage Stability | Organism |
---|---|
-20°C, stable for more than one month | Colwellia maris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + acetyl-CoA + H2O | - |
Colwellia maris | (S)-malate + CoA | - |
? | |
glyoxylate + acetyl-CoA + H2O | enzyme specifically involved in glyoxalate cycle metabolism | Colwellia maris | (S)-malate + CoA | - |
? | |
glyoxylate + acetyl-CoA + H2O | enzyme essential for acetate use in the bacterial cells | Colwellia maris | (S)-malate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 763000, SDS-PAGE | Colwellia maris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Colwellia maris |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Km for glyoxylate increases with decreasing temperature | Colwellia maris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
60.7 | - |
acetyl-CoA | at 20°C | Colwellia maris | |
161 | - |
acetyl-CoA | at 45°C | Colwellia maris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Colwellia maris |