Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pyruvate | - |
Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
gel filtration | Escherichia coli |
56000 | - |
gel filtration | Bacillus licheniformis |
56000 | - |
gel filtration | Vogesella indigofera |
56000 | - |
1 * 56000, SDS-PAGE | Escherichia coli |
58000 | - |
1 * 58000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
62000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Bacillus sp. (in: Bacteria) | - |
thermophilic | - |
Escherichia coli | - |
- |
- |
Vogesella indigofera | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Bacillus sp. (in: Bacteria) |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Bacillus sp. (in: Bacteria) | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26.9 | - |
wild-type enzyme | Bacillus sp. (in: Bacteria) |
27 | - |
mutant enzyme PC2 NG35 | Bacillus sp. (in: Bacteria) |
34.3 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + acetyl-CoA + H2O | - |
Escherichia coli | (S)-malate + CoA | - |
? | |
glyoxylate + acetyl-CoA + H2O | - |
Bacillus sp. (in: Bacteria) | (S)-malate + CoA | - |
? | |
glyoxylate + acetyl-CoA + H2O | - |
Bacillus licheniformis | (S)-malate + CoA | - |
? | |
glyoxylate + acetyl-CoA + H2O | - |
Vogesella indigofera | (S)-malate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 58000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
monomer | 1 * 56000, SDS-PAGE | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
50 mM Tris-HCl, pH 8.0, without KCl or with 0.2 M KCl, half-life: 25 min | Escherichia coli |
45 | - |
pH 7.5-9.5, completely stable for at least 2 h | Bacillus sp. (in: Bacteria) |
50 | - |
50 mM Tris-HCL, pH 8.0, without KCl or with 0.2 M KCl, half-life: 3 min | Escherichia coli |
60 | - |
25 mM Tris-HCl, pH 7.0, without KCl, half-life: 168 min, 25 mM Tris-HCL, pH 7.0, with KCl, half-life: 30 min, 25 mM glycine-NaOH buffer, pH 8.5, without KCl, half-life: 750 min, 25 mM glycine-NaOH buffer, pH 8.5, with 0.2 KCl, half-life: 50 min, 25 mM glycine-NaOH buffer, pH 9.0, without KCl, half-life: 522 min, 25 mM glycine-NaOH buffer, pH 9.0, with 0.2 KCl, half-life: 12 min, 25 mM Tris-HCL, pH 6.86, with 0.2 M KCl, half-life: 2 min | Bacillus sp. (in: Bacteria) |