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Literature summary for 2.3.2.21 extracted from
- Mutations of residues in pocket P1 of a cyclodipeptide synthase strongly increase product formation (2017), J. Nat. Prod., 80, 2917-2922 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli resulted in the formation of cyclo-(L-Tyr-L-Tyr) as the minor and cyclo-(L-Tyr-L-Phe) as the major products | Nocardiopsis prasina |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| T82A | site-directed mutagenesis, cYY/cYF product ratio compared to wild-type enzyme | Nocardiopsis prasina |
| T82A/Y196F | site-directed mutagenesis, cYY/cYF product ratio compared to wild-type enzyme | Nocardiopsis prasina |
| T82A/Y196L | site-directed mutagenesis, cYY/cYF product ratio compared to wild-type enzyme | Nocardiopsis prasina |
| T82V | site-directed mutagenesis, cYY/cYF product ratio compared to wild-type enzyme | Nocardiopsis prasina |
| T82V/Y196F | site-directed mutagenesis, an 8fold increase in product formation for cyclo(L-tyrosyl-L-tyrosyl) and 10fold for cyclo(L-tyrosyl-L-phenylalanyl) are detected in the double mutant compared to the wild-type, cYY/cYF product ratio compared to wild-type enzyme | Nocardiopsis prasina |
| T82V/Y196L | site-directed mutagenesis, cYY/cYF product ratio compared to wild-type enzyme | Nocardiopsis prasina |
| Y196F | site-directed mutagenesis, cYY/cYF product ratio compared to wild-type enzyme | Nocardiopsis prasina |
| Y196L | site-directed mutagenesis, cYY/cYF product ratio compared to wild-type enzyme | Nocardiopsis prasina |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-tyrosyl-tRNATyr | Nocardiopsis prasina | - |
tRNATyr + cyclo(L-tyrosyl-L-tyrosyl) | - |
? |  |
| L-tyrosyl-tRNATyr + L-phenylalanyl-tRNAPhe | Nocardiopsis prasina | - |
tRNATyr + tRNAPhe + cyclo(L-tyrosyl-L-phenylalanyl) | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nocardiopsis prasina | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography | Nocardiopsis prasina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-tyrosyl-tRNATyr | - |
Nocardiopsis prasina | 2 tRNATyr + cyclo(L-tyrosyl-L-tyrosyl) | - |
? | |
| 2 L-tyrosyl-tRNATyr | - |
Nocardiopsis prasina | tRNATyr + cyclo(L-tyrosyl-L-tyrosyl) | - |
? | |
| L-tyrosyl-tRNATyr + L-phenylalanyl-tRNAPhe | - |
Nocardiopsis prasina | tRNATyr + tRNAPhe + cyclo(L-tyrosyl-L-phenylalanyl) | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDPS | - |
Nocardiopsis prasina |
| CDPS-Np | - |
Nocardiopsis prasina |
| cyclodipeptide synthase | - |
Nocardiopsis prasina |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
in vivo assay at | Nocardiopsis prasina |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the CDPS family, NYH subfamily containing the catalytic residues Asn40, Tyr178, and His203 (AlbC numbering). The exception of Tyr196, CDPS-Np shares identical amino acids in other positions with one or more CDPSs in pocket P1 | Nocardiopsis prasina |
| additional information | strong influence on product accumulation of the amino acid residues in pocket P1 | Nocardiopsis prasina |
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Release 2023.1 (January 2023)
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