Literature summary for 2.3.2.15 extracted from

Park, H.C.; Hwang, J.E.; Jiang, Y.; Kim, Y.J.; Kim, S.H.; Nguyen, X.C.; Kim, C.Y.; Chung, W.S.
Functional characterisation of two phytochelatin synthases in rice (Oryza sativa cv. Milyang 117) that respond to cadmium stress (2019), Plant Biol., 21, 854-861 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene PCS15, DNA and amino acid sequence determinataion and analysis, RT-PCR expression analysis, recombinant expression in Saccharomyces cerevisiae ycf1 mutant (DELTAycf1) cells (DTY167) confers Cd resistance. The growth of OsPCS15-transformed DELTAycf1 cells is similar to that of empty vector-transformed wild-type and DELTAycf1 cells. Recombinant overexpression of GFP-tagged enzyme PCS15 in Arabidopsis thaliana under control of the CaMV 35S promoter leading to increasing Cd2+ concentrations. Root growth in OsPCS15 transgenic plants is reduced by approximately 26% following treatment with 0.05-0.07 mM CdCl2. In contrast, ectopic expression of PCS15 increases the sensitivity to Cd in Arabidopsis thaliana	Oryza sativa
gene PCS5, DNA and amino acid sequence determinataion and analysis, RT-PCR expression analysis, recombinant expression in Saccharomyces cerevisiae ycf1 mutant (DELTAycf1) cells (DTY167) confers Cd resistance. The growth of OsPCS5-transformed DELTAycf1 cells is similar to that of empty vector-transformed wild-type and DELTAycf1 cells. Recombinant overexpression of GFP-tagged enzyme PCS5 in Arabidopsis thaliana under control of the CaMV 35S promoter leading to increasing Cd2+ concentrations. Root growth in OsPCS5 transgenic plants is reduced by approximately 33% following treatment with 0.05-0.07 mM CdCl2. In contrast, ectopic expression of PCS5 increases the sensitivity to Cd in Arabidopsis thaliana	Oryza sativa

Cloned (Comment)

Organism

gene PCS15, DNA and amino acid sequence determinataion and analysis, RT-PCR expression analysis, recombinant expression in Saccharomyces cerevisiae ycf1 mutant (DELTAycf1) cells (DTY167) confers Cd resistance. The growth of OsPCS15-transformed DELTAycf1 cells is similar to that of empty vector-transformed wild-type and DELTAycf1 cells. Recombinant overexpression of GFP-tagged enzyme PCS15 in Arabidopsis thaliana under control of the CaMV 35S promoter leading to increasing Cd2+ concentrations. Root growth in OsPCS15 transgenic plants is reduced by approximately 26% following treatment with 0.05-0.07 mM CdCl2. In contrast, ectopic expression of PCS15 increases the sensitivity to Cd in Arabidopsis thaliana

Oryza sativa

gene PCS5, DNA and amino acid sequence determinataion and analysis, RT-PCR expression analysis, recombinant expression in Saccharomyces cerevisiae ycf1 mutant (DELTAycf1) cells (DTY167) confers Cd resistance. The growth of OsPCS5-transformed DELTAycf1 cells is similar to that of empty vector-transformed wild-type and DELTAycf1 cells. Recombinant overexpression of GFP-tagged enzyme PCS5 in Arabidopsis thaliana under control of the CaMV 35S promoter leading to increasing Cd2+ concentrations. Root growth in OsPCS5 transgenic plants is reduced by approximately 33% following treatment with 0.05-0.07 mM CdCl2. In contrast, ectopic expression of PCS5 increases the sensitivity to Cd in Arabidopsis thaliana

Oryza sativa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Oryza sativa	5829	-

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell suspension culture	-	Oryza sativa	-
additional information	PCS15 expression pattern in rice, overview	Oryza sativa	-
root	-	Oryza sativa	-

Synonyms

Synonyms	Comment	Organism
OsPCS15	-	Oryza sativa
OsPCS5	-	Oryza sativa
PCS	-	Oryza sativa
PCS15	-	Oryza sativa
PCS5	-	Oryza sativa
phytochelatin synthase	-	Oryza sativa

Expression

Organism	Comment	Expression
Oryza sativa	expression of PCS15 is induced by Cd2+ and As5+ ions	up
Oryza sativa	expression of PCS5 is induced by Cd2+ and As5+ ions	up