Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GSH | the maximal activity is attained at GSH concentrations around 10 and 15 mM when the enzyme is assayed in the presence of 0.005 and 0.01 mM total Cd2+, respectively | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Rosetta (DE3) pLysS cells | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | the enzyme also possesses a second Cd2+ binding site where Cd2+ binds to induce an inhibitory effect | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | the enzyme possesses two Cd2+ binding sites, Cd2+ binds to one of these sites to activate the enzyme. In the presence of 0.01 mM Cd2+, activity increases and reaches the maximum rate with an increase in GSH concentration up to 15 mM | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56500 | - |
x * 56500, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + [Glu(-Cys)]n-Gly | Arabidopsis thaliana | - |
Gly + [Glu(-Cys)]n+1-Gly | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ affinity column chromatography and HisTrap HP column chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + [Glu(-Cys)]n-Gly | - |
Arabidopsis thaliana | Gly + [Glu(-Cys)]n+1-Gly | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56500, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
gamma-glutamylcysteinyl transpeptidase | - |
Arabidopsis thaliana |
PCS1 | - |
Arabidopsis thaliana |
phytochelatin synthase | - |
Arabidopsis thaliana |