Literature summary for 2.3.1.51 extracted from

Zhang, Q.Y.; Niu, L.X.; Yu, R.; Zhang, X.X.; Bai, Z.Z.; Duan, K.; Gao, Q.H.; Zhang, Y.L.
Cloning, characterization, and expression analysis of a gene encoding a putative lysophosphatidic acid acyltransferase from seeds of Paeonia rockii (2017), Appl. Biochem. Biotechnol., 182, 721-741 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene PrLPAAT1, DNA and amino acid sequence determination and analysis, domain structure, phylogenetic analysis, quantitative real-time RT-PCR enzyme expression analysis, recombinant overexpression using a seed-specific promoter in Arabidopsis thaliana plants, recombinant expression of GFP-tagged enzyme in the lower epidermis of Nicotiana tabacum leaves by Agrobacterium-mediated transformation method	Paeonia rockii

Cloned (Comment)

Organism

gene PrLPAAT1, DNA and amino acid sequence determination and analysis, domain structure, phylogenetic analysis, quantitative real-time RT-PCR enzyme expression analysis, recombinant overexpression using a seed-specific promoter in Arabidopsis thaliana plants, recombinant expression of GFP-tagged enzyme in the lower epidermis of Nicotiana tabacum leaves by Agrobacterium-mediated transformation method

Paeonia rockii

Protein Variants

Protein Variants	Comment	Organism
additional information	recombinant overexpression of gene PrLPAAT1 in Arabidopsis thaliana transgenic plant seeds increases total fatty acid (FA) content and the main fatty acid accumulation. Most of the FAs have higher levels of production in the transgenic T1 seeds compared to wild-type seeds, and the predominant FAs in the transgenic seeds are C18:1, C18:2, C18:3, C20:1, and C16:0	Paeonia rockii

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	subcelluar localization study in transgenic Nicotiana tabacum leaves	Paeonia rockii	-	-
plasma membrane	the enzyme contains two transmembrane domains	Paeonia rockii	5886	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate	Paeonia rockii	enzyme LPAAT catalyzes lysophosphatidic acid to yield phosphatidic acid by acylation of the sn-2 position of glycerol 3-phosphate. LPAAT is able to discriminate acyl groups with different chain lengths and possesses a selectivity and specificity for unsaturated C18 acyl groups in traditional oil seed crops	CoA + 1,2-diacyl-sn-glycerol 3-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Paeonia rockii	KX256278	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
flower	-	Paeonia rockii	-
additional information	PrLPAAT1 is ubiquitously expressed in diverse tissues, the expression is higher in the flower and developing seed, isozyme expression profile and fatty acid contents in different organs as well as in seeds at different developmental stages, overview	Paeonia rockii	-
seed	analysis of enzyme expression during seed development, overview. PrLPAAT1 expression has a biphasic pattern. PrLPAAT1 exhibits relatively high transcript abundance during S1-S6 and drops thereafter (S6-S9). The expression of the PrLPAAT1 gene is increased at S1-S6, which coincides with the highest rate of fatty acid accumulation in tree peony seeds	Paeonia rockii	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate	-	Paeonia rockii	CoA + 1,2-diacyl-sn-glycerol 3-phosphate	-	?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate	enzyme LPAAT catalyzes lysophosphatidic acid to yield phosphatidic acid by acylation of the sn-2 position of glycerol 3-phosphate. LPAAT is able to discriminate acyl groups with different chain lengths and possesses a selectivity and specificity for unsaturated C18 acyl groups in traditional oil seed crops	Paeonia rockii	CoA + 1,2-diacyl-sn-glycerol 3-phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 38800, about, sequence calculation	Paeonia rockii
More	conserved domain analysis, and PrLPAAT1 tertiary structural modelling. The enzyme contains conserved 1-acyl-sn-glycerol-3-phosphate acyltransferase-related (PLN02901) and LPLAT_AGPAT-like (cd07989) domain	Paeonia rockii

Synonyms

Synonyms	Comment	Organism
acyl-CoA:1-acylglycerol-sn-3-phosphate acyltransferase	-	Paeonia rockii
AGPAT	-	Paeonia rockii
LPAAT	-	Paeonia rockii
lysophosphatidic acid acyltransferase	-	Paeonia rockii
PrLPAAT1	-	Paeonia rockii

pI Value

Organism	Comment	pI Value Maximum	pI Value
Paeonia rockii	sequence calculation	-	9.68

General Information

General Information	Comment	Organism
evolution	PrLPAAT1 may be a member of AGPAT family, and may have acyltransferase activity, comparisons of sequences, genetic structures, and conserved structure motifs, overview. Phylogenetic analysis	Paeonia rockii
metabolism	through the conventional Kennedy pathway in the endoplasmic reticulum, triacylglycerol (TAG) is synthesized de novo in three acylation reactions at the sn-1-, sn-2-, and sn-3-positions of the glycerol-3-phosphate backbone with acyl chains from acyl-CoAs, and these three reactions respectively are catalyzed by glycerol-3-phosphate acyltransferase (GPAT, EC 2.3.1.15), LPAAT, and diacylglycerol acyltransferase (DGAT, EC 2.3.1.20)	Paeonia rockii
additional information	PrLPAAT1 structure prediction and modelling, overview	Paeonia rockii
physiological function	PrLPAAT1 is an important component in the fatty acid accumulation process, especially during the early stages of seed development. LPAAT is able to discriminate acyl groups with different chain lengths and possesses a selectivity and specificity for unsaturated C18 acyl groups in traditional oil seed crops	Paeonia rockii