Literature summary for 2.3.1.304 extracted from

Tan, H.T.; Chek, M.F.; Lakshmanan, M.; Foong, C.P.; Hakoshima, T.; Sudesh, K.
Evaluation of BP-M-CPF4 polyhydroxyalkanoate (PHA) synthase on the production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil using Cupriavidus necator transformants (2020), Int. J. Biol. Macromol., 159, 250-257 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Cupriavidus necator strains PHB-4, Re2058 and Re2160 and Escherichia coli Rosetta2 (DE3) cells	Chromobacterium sp. USM2

Organism

Organism	UniProt	Comment	Textmining
Chromobacterium sp. USM2	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography, and HiTrap Q anion-exchange column chromatography	Chromobacterium sp. USM2

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(3R)-3-hydroxybutyryl-CoA + poly[(R)-3-hydroxybutyrate]n	-	Chromobacterium sp. USM2	CoA + poly[(R)-3-hydroxybutyrate]n+1	-	?

Subunits

Subunits	Comment	Organism
?	x * 62000, recombinant enzyme, SDS-PAGE	Chromobacterium sp. USM2

Synonyms

Synonyms	Comment	Organism
PHA synthase	-	Chromobacterium sp. USM2
phaCBP-M-CPF4	-	Chromobacterium sp. USM2
polyhydroxyalkanoate synthase	-	Chromobacterium sp. USM2

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Release 2023.1 (January 2023)