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Literature summary for 2.3.1.304 extracted from

  • Mezzolla, V.; D'Urso, O.; Poltronieri, P.
    Role of PhaC type I and type II enzymes during PHA biosynthesis (2018), Polymers (Basel), 10, 910 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.304

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas aeruginosa Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors ?
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additional information Pseudomonas putida Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors ?
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additional information Pseudomonas mendocina Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors ?
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additional information Pseudomonas oleovorans Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors ?
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additional information Pseudomonas stutzeri Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors ?
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Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q51513 gene PhaC1
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Pseudomonas mendocina Q2PMY5 gene PhaC1
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Pseudomonas oleovorans P26494 gene PhaC1
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Pseudomonas putida A0A1Y5KN65 PhaC1 or gene phbC_1
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Pseudomonas stutzeri Q848R9 gene PhaC2
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors Pseudomonas aeruginosa ?
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-
additional information Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors Pseudomonas putida ?
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-
additional information Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors Pseudomonas mendocina ?
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additional information Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors Pseudomonas oleovorans ?
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additional information Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors Pseudomonas stutzeri ?
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Subunits

Subunits Comment Organism
More Classes I and II PHA synthases are formed by a single protein (PhaC), of about 60 kDa Pseudomonas aeruginosa
More Classes I and II PHA synthases are formed by a single protein (PhaC), of about 60 kDa Pseudomonas putida
More Classes I and II PHA synthases are formed by a single protein (PhaC), of about 60 kDa Pseudomonas mendocina
More Classes I and II PHA synthases are formed by a single protein (PhaC), of about 60 kDa Pseudomonas oleovorans
More Classes I and II PHA synthases are formed by a single protein (PhaC), of about 60 kDa Pseudomonas stutzeri

Synonyms

Synonyms Comment Organism
Class II PHA polymerizing enzyme
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 Pseudomonas aeruginosa
Class II PHA polymerizing enzyme
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 Pseudomonas putida
Class II PHA polymerizing enzyme
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 Pseudomonas mendocina
Class II PHA polymerizing enzyme
-
 Pseudomonas oleovorans
Class II PHA polymerizing enzyme
-
 Pseudomonas stutzeri
class II PHA synthase
-
 Pseudomonas aeruginosa
class II PHA synthase
-
 Pseudomonas putida
class II PHA synthase
-
 Pseudomonas mendocina
class II PHA synthase
-
 Pseudomonas oleovorans
class II PHA synthase
-
 Pseudomonas stutzeri
Class II PhaC
-
 Pseudomonas aeruginosa
Class II PhaC
-
 Pseudomonas putida
Class II PhaC
-
 Pseudomonas mendocina
Class II PhaC
-
 Pseudomonas oleovorans
Class II PhaC
-
 Pseudomonas stutzeri
PhaC type II
-
 Pseudomonas aeruginosa
PhaC type II
-
 Pseudomonas putida
PhaC type II
-
 Pseudomonas mendocina
PhaC type II
-
 Pseudomonas oleovorans
PhaC type II
-
 Pseudomonas stutzeri
PhaC-II
-
 Pseudomonas putida
phbC_2
-
 Pseudomonas mendocina

General Information

General Information Comment Organism
evolution PhaC synthases are grouped into four classes based on substrate specificity, and the preference in forming short-chain-length (scl) or medium-chain-length (mcl) polymers: Class I, Class III and Class IV produce principally scl-PHAs, while Class II PhaC synthesize mcl-PHAs. Class II PhaC synthases are widely distributed in bacteria. Class II PhaCPhaC enzymes differ from PhaCCn, as prototype of Class I PHA synthases, by about 28 amino acids, reaching the C-terminal (1-559) with a sequence shorter by about 30 amino acids. The catalytic triad has been renumbered as Cys296, Asp452, His453 and His480 in Pseudomonas spp., prototype for Class II PHA synthases. Tyr412 in PhaCCs, and Tyr446 in the 6-helix in PhaCCn, are residues conserved in Class I, III and IV PHA synthases, while Phe occupies this position in Class II synthases Pseudomonas aeruginosa
evolution PhaC synthases are grouped into four classes based on substrate specificity, and the preference in forming short-chain-length (scl) or medium-chain-length (mcl) polymers: Class I, Class III and Class IV produce principally scl-PHAs, while Class II PhaC synthesize mcl-PHAs. Class II PhaC synthases are widely distributed in bacteria. Class II PhaCPhaC enzymes differ from PhaCCn, as prototype of Class I PHA synthases, by about 28 amino acids, reaching the C-terminal (1-559) with a sequence shorter by about 30 amino acids. The catalytic triad has been renumbered as Cys296, Asp452, His453 and His480 in Pseudomonas spp., prototype for Class II PHA synthases. Tyr412 in PhaCCs, and Tyr446 in the 6-helix in PhaCCn, are residues conserved in Class I, III and IV PHA synthases, while Phe occupies this position in Class II synthases Pseudomonas putida
evolution PhaC synthases are grouped into four classes based on substrate specificity, and the preference in forming short-chain-length (scl) or medium-chain-length (mcl) polymers: Class I, Class III and Class IV produce principally scl-PHAs, while Class II PhaC synthesize mcl-PHAs. Class II PhaC synthases are widely distributed in bacteria. Class II PhaCPhaC enzymes differ from PhaCCn, as prototype of Class I PHA synthases, by about 28 amino acids, reaching the C-terminal (1-559) with a sequence shorter by about 30 amino acids. The catalytic triad has been renumbered as Cys296, Asp452, His453 and His480 in Pseudomonas spp., prototype for Class II PHA synthases. Tyr412 in PhaCCs, and Tyr446 in the 6-helix in PhaCCn, are residues conserved in Class I, III and IV PHA synthases, while Phe occupies this position in Class II synthases Pseudomonas mendocina
evolution PhaC synthases are grouped into four classes based on substrate specificity, and the preference in forming short-chain-length (scl) or medium-chain-length (mcl) polymers: Class I, Class III and Class IV produce principally scl-PHAs, while Class II PhaC synthesize mcl-PHAs. Class II PhaC synthases are widely distributed in bacteria. Class II PhaCPhaC enzymes differ from PhaCCn, as prototype of Class I PHA synthases, by about 28 amino acids, reaching the C-terminal (1-559) with a sequence shorter by about 30 amino acids. The catalytic triad has been renumbered as Cys296, Asp452, His453 and His480 in Pseudomonas spp., prototype for Class II PHA synthases. Tyr412 in PhaCCs, and Tyr446 in the 6-helix in PhaCCn, are residues conserved in Class I, III and IV PHA synthases, while Phe occupies this position in Class II synthases Pseudomonas oleovorans
evolution PhaC synthases are grouped into four classes based on substrate specificity, and the preference in forming short-chain-length (scl) or medium-chain-length (mcl) polymers: Class I, Class III and Class IV produce principally scl-PHAs, while Class II PhaC synthesize mcl-PHAs. Class II PhaC synthases are widely distributed in bacteria. Class II PhaCPhaC enzymes differ from PhaCCn, as prototype of Class I PHA synthases, by about 28 amino acids, reaching the C-terminal (1-559) with a sequence shorter by about 30 amino acids. The catalytic triad has been renumbered as Cys296, Asp452, His453 and His480 in Pseudomonas spp., prototype for Class II PHA synthases. Tyr412 in PhaCCs, and Tyr446 in the 6-helix in PhaCCn, are residues conserved in Class I, III and IV PHA synthases, while Phe occupies this position in Class II synthases Pseudomonas stutzeri
additional information the region Leu402-Asn415 forming the alpha4-helix in PhaCCn-CAT is conserved among Class I and II PHA synthases, whereas the corresponding segment, Leu369-Lys382 of PhaCCs-CAT, displays a disordered structure. Catalytic mechanism, overview Pseudomonas aeruginosa
additional information the region Leu402-Asn415 forming the alpha4-helix in PhaCCn-CAT is conserved among Class I and II PHA synthases, whereas the corresponding segment, Leu369-Lys382 of PhaCCs-CAT, displays a disordered structure. Catalytic mechanism, overview Pseudomonas putida
additional information the region Leu402-Asn415 forming the alpha4-helix in PhaCCn-CAT is conserved among Class I and II PHA synthases, whereas the corresponding segment, Leu369-Lys382 of PhaCCs-CAT, displays a disordered structure. Catalytic mechanism, overview Pseudomonas mendocina
additional information the region Leu402-Asn415 forming the alpha4-helix in PhaCCn-CAT is conserved among Class I and II PHA synthases, whereas the corresponding segment, Leu369-Lys382 of PhaCCs-CAT, displays a disordered structure. Catalytic mechanism, overview Pseudomonas oleovorans
additional information the region Leu402-Asn415 forming the alpha4-helix in PhaCCn-CAT is conserved among Class I and II PHA synthases, whereas the corresponding segment, Leu369-Lys382 of PhaCCs-CAT, displays a disordered structure. Catalytic mechanism, overview Pseudomonas stutzeri
physiological function Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors, Class II PhaC enzymes synthesize mcl-polymers depending on 3-hydroxyhexanoate (3HH), 3-hydroxyheptanoate (3HHp), 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD), 3-hydroxyundecanoate (3HUD), 3-hydroxydodecanoate (3HDD) (C6 to C12), and availability of the corresponding CoA thioester substrates, originating from three different metabolic pathways. In Pseudomonas spp., there are two PhaC genes, of which PhaC1 is the active enzyme under physiological conditions Pseudomonas oleovorans
physiological function Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors, Class II PhaC enzymes synthesize mcl-polymers depending on 3-hydroxyhexanoate (3HH), 3-hydroxyheptanoate (3HHp), 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD), 3-hydroxyundecanoate (3HUD), 3-hydroxydodecanoate (3HDD) (C6 to C12), and availability of the corresponding CoA thioester substrates, originating from three different metabolic pathways. In Pseudomonas spp., there are two PhaC genes, of which PhaC1 is the active enzyme under physiological conditions Pseudomonas stutzeri
physiological function Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors, Class II PhaC enzymes synthesize mcl-polymers depending on 3-hydroxyhexanoate (3HH), 3-hydroxyheptanoate (3HHp), 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD), 3-hydroxyundecanoate (3HUD), 3-hydroxydodecanoate (3HDD) (C6 to C12), and availability of the corresponding CoA thioester substrates, originating from three different metabolic pathways. In Pseudomonas spp., there are two PhaCPhaC genes, of which PhaC1 is the active enzyme under physiological conditions Pseudomonas aeruginosa
physiological function Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors, Class II PhaC enzymes synthesize mcl-polymers depending on 3-hydroxyhexanoate (3HH), 3-hydroxyheptanoate (3HHp), 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD), 3-hydroxyundecanoate (3HUD), 3-hydroxydodecanoate (3HDD) (C6 to C12), and availability of the corresponding CoA thioester substrates, originating from three different metabolic pathways. In Pseudomonas spp., there are two PhaCPhaC genes, of which PhaC1 is the active enzyme under physiological conditions Pseudomonas putida
physiological function Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors, Class II PhaC enzymes synthesize mcl-polymers depending on 3-hydroxyhexanoate (3HH), 3-hydroxyheptanoate (3HHp), 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD), 3-hydroxyundecanoate (3HUD), 3-hydroxydodecanoate (3HDD) (C6 to C12), and availability of the corresponding CoA thioester substrates, originating from three different metabolic pathways. In Pseudomonas spp., there are two PhaCPhaC genes, of which PhaC1 is the active enzyme under physiological conditions Pseudomonas mendocina