Literature summary for 2.3.1.304 extracted from

Thomson, N.; Saika, A.; Ushimaru, K.; Sangiambut, S.; Tsuge, T.; Summers, D.; Sivaniah, E.
Efficient production of active polyhydroxyalkanoate synthase in Escherichia coli by coexpression of molecular chaperones (2013), Appl. Environ. Microbiol., 79, 1948-1955.

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Application

Application	Comment	Organism
synthesis	heterologous expression in Escherichia coli with simultaneous overexpression of chaperone proteins. Compared to expression of synthase alone (14.55 mg per liter), coexpression with chaperones results in the production of larger total quantities of enzyme, including a larger proportion in the soluble fraction. The largest increase is seen when the GroEL/GroES system is coexpressed, resulting in approximately 6fold greater enzyme yields (82.37 mg per liter) than in the absence of coexpressed chaperones. The specific activity of the purified enzyme is unaffected by coexpression with chaperones	Cupriavidus necator

Cloned(Commentary)

Cloned (Comment)	Organism
exypression in Escherichia coli	Cupriavidus necator

Organism

Organism	UniProt	Comment	Textmining
Cupriavidus necator	P23608	-	-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1	P23608	-	-

Purification (Commentary)

Purification (Comment)	Organism
one-step purification protocol	Cupriavidus necator

Synonyms

Synonyms	Comment	Organism
PhaC	-	Cupriavidus necator

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Release 2023.1 (January 2023)