Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.304 extracted from

  • Sato, S.; Minato, M.; Kikkawa, Y.; Abe, H.; Tsuge, T.
    In vitro synthesis of polyhydroxyalkanoate catalyzed by class II and III PHA synthases: A useful technique for surface coatings of a hydrophobic support with PHA (2010), J. Chem. Technol. Biotechnol., 85, 779-782.
No PubMed abstract available
Search for more literature for 2.3.1.304

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged PhaC1Pp in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Pseudomonas putida
recombinant expression of His-tagged PhaECAv in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Allochromatium vinosum

Protein Variants

Protein Variants Comment Organism
additional information the enzyme is used for in vitro synthesis of polyhydroxyalkanoates on a hydrophobic support, i.e. highly oriented pyrolytic graphite. Using PhaC1Pp and 3-hydroxyoctanoyl-CoA at room temperature, a poly(3-hydroxyoctanoate) [P(3HO)] film is formed on the hydrophobic support with a thickness of a few nanometers, as revealed by atomic force microscopy Pseudomonas putida
additional information the enzyme is used for in vitro synthesis of polyhydroxyalkanoates on a hydrophobic support, i.e. highly oriented pyrolytic graphite. Using PhaECAv and 3-hydroxyoctanoyl-CoA at room temperature, a poly(3-hydroxyoctanoate) [P(3HO)] film is formed on the hydrophobic support with a thickness of a few nanometers, as revealed by atomic force microscopy Allochromatium vinosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n Pseudomonas putida
-
 [(R)-3-hydroxyacyl]n+1 + CoA
-
 ?
3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n Allochromatium vinosum
-
 [(R)-3-hydroxyacyl]n+1 + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Allochromatium vinosum
-
-
-
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged PhaC1Pp from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Pseudomonas putida
recombinant His-tagged PhaECAv from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Allochromatium vinosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n
-
 Pseudomonas putida [(R)-3-hydroxyacyl]n+1 + CoA
-
 ?
3-hydroxyacyl-CoA + [(R)-3-hydroxyacyl]n
-
 Allochromatium vinosum [(R)-3-hydroxyacyl]n+1 + CoA
-
 ?

Synonyms

Synonyms Comment Organism
class II PHA synthase
-
 Pseudomonas putida
class III PHA synthase
-
 Allochromatium vinosum
PhaC1Pp
-
 Pseudomonas putida
PhaECAv
-
 Allochromatium vinosum