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Literature summary for 2.3.1.304 extracted from

  • Hezayen, F.F.; Steinbuchel, A.; Rehm, B.H.
    Biochemical and enzymological properties of the polyhydroxybutyrate synthase from the extremely halophilic archaeon strain 56 (2002), Arch. Biochem. Biophys., 403, 284-291.
    View publication on PubMed
Search for more literature for 2.3.1.304

Inhibitors

Inhibitors Comment Organism Structure
CoA
-
 Halopiger aswanensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056
-
 (R)-3-hydroxybutyryl-CoA
-
 Halopiger aswanensis

Localization

Localization Comment Organism GeneOntology No. Textmining
poly(3-hydroxybutyrate) granule PHB synthase can not be released from the PHB granules, indicating a covalent attachment to the PHB core Halopiger aswanensis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ addition of Mg2+ shows the strongest activation effect. However, high enzyme activity without Mg2+ ions is observed, when other ions are present, suggesting that Mg2+ ions do not serve as cofactors Halopiger aswanensis
additional information in the absence of salts the granule-bound PHB synthase still exhibits about 40% of the maximum activity. Enzyme activity is strongly enhanced by slightly increasing the ionic strength independent of the salt Halopiger aswanensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 x * 80000, SDS-PAGE Halopiger aswanensis

Organism

Organism UniProt Comment Textmining
Halopiger aswanensis
-
-
-
Halopiger aswanensis DSM 13151
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Halopiger aswanensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n PHB synthase exhibits positive cooperativity Halopiger aswanensis [(R)-3-hydroxybutanoate](n+1) + CoA
-
 ?
(R)-3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n PHB synthase exhibits positive cooperativity Halopiger aswanensis DSM 13151 [(R)-3-hydroxybutanoate](n+1) + CoA
-
 ?
additional information 3-hydroxyvaleryl-CoA, 4-hydroxybutyryl-CoA, and 3-hydroxydecanoyl-CoA are not accepted as substrates Halopiger aswanensis ?
-
 ?
additional information 3-hydroxyvaleryl-CoA, 4-hydroxybutyryl-CoA, and 3-hydroxydecanoyl-CoA are not accepted as substrates Halopiger aswanensis DSM 13151 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Halopiger aswanensis

Synonyms

Synonyms Comment Organism
PHB synthase
-
 Halopiger aswanensis
polyhydroxybutyrate synthase
-
 Halopiger aswanensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 60
-
 Halopiger aswanensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 20°C: about 50% of maximal activity, 70°C: about 50% of maximal activity Halopiger aswanensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable up to Halopiger aswanensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Halopiger aswanensis

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 pH 6.5: about 30% of maximal activity, pH 8.5: about 85% of maximal activity Halopiger aswanensis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.16
-
-
 Halopiger aswanensis CoA