Literature summary for 2.3.1.304 extracted from

Hai, T.; Lange, D.; Rabus, R.; Steinbuchel, A.
Polyhydroxyalkanoate (PHA) accumulation in sulfate-reducing bacteria and identification of a class III PHA synthase (PhaEC) in Desulfococcus multivorans (2004), Appl. Environ. Microbiol., 70, 4440-4448.

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Cloned(Commentary)

Cloned (Comment)	Organism
constructs of phaCDm alone (pBBRMCS-2::phaCDm) and of phaEDmCDm (pBBRMCS-2::phaEDmCDm) in various vectors are obtained and transferred to several strains of Escherichia coli, as well as to the PHA-negative mutants PHB-4 and GPp104 of Ralstonia eutropha and Pseudomonas putida, respectively. In cells of the recombinant strains harboring phaEDmCDm small but significant amounts (up to 1.7% of cell dry matter) of poly(3-hydroxybutyrate) and of PHA synthase activity (up to 1.5 U/mg protein) are detected. Hybrid synthases consisting of PhaCDm and PhaE of Thiococcus pfennigii or Synechocystis sp. strain PCC 6308 are also constructed and are shown to be functionally active	Desulfococcus multivorans

Cloned (Comment)

Organism

constructs of phaCDm alone (pBBRMCS-2::phaCDm) and of phaEDmCDm (pBBRMCS-2::phaEDmCDm) in various vectors are obtained and transferred to several strains of Escherichia coli, as well as to the PHA-negative mutants PHB-4 and GPp104 of Ralstonia eutropha and Pseudomonas putida, respectively. In cells of the recombinant strains harboring phaEDmCDm small but significant amounts (up to 1.7% of cell dry matter) of poly(3-hydroxybutyrate) and of PHA synthase activity (up to 1.5 U/mg protein) are detected. Hybrid synthases consisting of PhaCDm and PhaE of Thiococcus pfennigii or Synechocystis sp. strain PCC 6308 are also constructed and are shown to be functionally active

Desulfococcus multivorans

Organism

Organism	UniProt	Comment	Textmining
Desulfococcus multivorans	-	-	-

Synonyms

Synonyms	Comment	Organism
class III PHA synthase	-	Desulfococcus multivorans
PhaEC	-	Desulfococcus multivorans

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Release 2023.1 (January 2023)