Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Thermus thermophilus HB8 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1.0 mM, complete inhibition | Thermus thermophilus HB8 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
acetyl-CoA | pH 7.8, 30°C | Thermus thermophilus HB8 | |
0.013 | - |
L-serine | pH 7.8, 30°C | Thermus thermophilus HB8 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.1 mM, 31.7fold activation | Thermus thermophilus HB8 | |
Fe2+ | enzyme contains Zn2+ and Fe2+ | Thermus thermophilus HB8 | |
Ni2+ | 0.1 mM, 12fold activation | Thermus thermophilus HB8 | |
Zn2+ | enzyme contains Zn2+ and Fe2+ | Thermus thermophilus HB8 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, SDS-PAGE | Thermus thermophilus HB8 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-Ser | Thermus thermophilus HB8 | key enzyme in L-cysteine biosynthesis | CoA + O-acetyl-L-Ser | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus HB8 | Q6F4D7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Thermus thermophilus HB8 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-Ser | - |
Thermus thermophilus HB8 | CoA + O-acetyl-L-Ser | - |
? | |
acetyl-CoA + L-Ser | key enzyme in L-cysteine biosynthesis | Thermus thermophilus HB8 | CoA + O-acetyl-L-Ser | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, SDS-PAGE | Thermus thermophilus HB8 |
Synonyms | Comment | Organism |
---|---|---|
L-serine O-acetyltransferase | - |
Thermus thermophilus HB8 |
SAT | - |
Thermus thermophilus HB8 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Thermus thermophilus HB8 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 80 | 45°C: about 50% of maximal activity, 80°C: about 55% of maximal activity | Thermus thermophilus HB8 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
L-serine | pH 7.8, 30°C | Thermus thermophilus HB8 | |
0.54 | - |
acetyl-CoA | pH 7.8, 30°C | Thermus thermophilus HB8 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Thermus thermophilus HB8 |