Cloned (Comment) | Organism |
---|---|
genes LAC1, LAG1, and LIP1. LIP1 DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of all three subunits as N-terminally FLAG- or HA-tagged proteins in Saccharomyces cerevisiae strains | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
australifungin | - |
Saccharomyces cerevisiae | |
fumonisin B1 | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | Lac1p and Lag1p are integral membrane proteins with six to eight predicted transmembrane domains. Co-subunit Lip1 is also an integral membrane protein, with one transmembrane domain. Its short N-terminal part is cytoplasmic and not required for ceramide synthesis. Analysis of membrane topology of the heterotrimer for ceramide synthase catalytic activity, overview | Saccharomyces cerevisiae | 5789 | - |
microsome | - |
Saccharomyces cerevisiae | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-erythro-sphinganine + arachidoyl-CoA | Saccharomyces cerevisiae | - |
N-arachidoyl-D-sphinganine + CoA | - |
? | |
D-erythro-sphinganine + arachidoyl-CoA | Saccharomyces cerevisiae RH4838 | - |
N-arachidoyl-D-sphinganine + CoA | - |
? | |
D-erythro-sphinganine + cerotoyl-CoA | Saccharomyces cerevisiae | - |
N-cerotoyl-D-sphinganine + CoA | - |
? | |
D-erythro-sphinganine + cerotoyl-CoA | Saccharomyces cerevisiae RH4838 | - |
N-cerotoyl-D-sphinganine + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P28496 AND P38703 AND Q03579 | encoding subunits Lac1p, Lag1p, and Lip1p | - |
Saccharomyces cerevisiae RH4838 | P28496 AND P38703 AND Q03579 | encoding subunits Lac1p, Lag1p, and Lip1p | - |
Purification (Comment) | Organism |
---|---|
native enzyme 3420fold subunits from isolated digitonin-treated membranes by solubilization with 1% Triton X-100 and affinity chromatography | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.68 | - |
purified native enzyme, pH 6.8, 24°C | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-erythro-sphinganine + arachidoyl-CoA | - |
Saccharomyces cerevisiae | N-arachidoyl-D-sphinganine + CoA | - |
? | |
D-erythro-sphinganine + arachidoyl-CoA | - |
Saccharomyces cerevisiae RH4838 | N-arachidoyl-D-sphinganine + CoA | - |
? | |
D-erythro-sphinganine + cerotoyl-CoA | - |
Saccharomyces cerevisiae | N-cerotoyl-D-sphinganine + CoA | - |
? | |
D-erythro-sphinganine + cerotoyl-CoA | - |
Saccharomyces cerevisiae RH4838 | N-cerotoyl-D-sphinganine + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | subunits Lac1p, Lag1p, and Lip1p | Saccharomyces cerevisiae |
More | Lag1p, Lac1p and Lip1p comprise the ceramide synthase. Lag1p/Lac1p interacting protein, 28 kDa, Lip1p is an essential component of the ceramide synthase, which forms a heteromeric complex with Lac1p and Lag1p. Lip1p is required for ceramide synthesis in vivo and in vitro. It is possible that Lip1p is modified by O-linked glycosylation. Its short, highly charged N-terminus is cytoplasmic and dispensible for ceramide synthase activity | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
acyl-CoA ceramide synthase | - |
Saccharomyces cerevisiae |
ceramide synthase complex | - |
Saccharomyces cerevisiae |
Lac1p/Lag1p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
physiological function | Lag1p, Lac1p and Lip1p comprise the ceramide synthase. Lag1p and Lac1p are subunits of the acyl-CoA-dependent ceramide synthase. The other essential component of the ceramide synthase, Lip1p, which forms a heteromeric complex with Lac1p and Lag1p. Lip1p is required for ceramide synthesis in vivo and in vitro | Saccharomyces cerevisiae |