Cloned (Comment) | Organism |
---|---|
expression of subunit AurA in Streptomyces lividans | Streptomyces thioluteus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of mutant protein complexes each lacking a critical PKS domain on subunit AurA domain for chain propagation | Streptomyces thioluteus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces thioluteus | Q70KH8 and Q70KH5 and Q70KH4 | Q70KH8 i.e. subunit AurA, Q70KH5 i.e. subunit AurB, Q70KH4 i.e. subunit AurC | - |
Streptomyces thioluteus HKI-227 | Q70KH8 and Q70KH5 and Q70KH4 | Q70KH8 i.e. subunit AurA, Q70KH5 i.e. subunit AurB, Q70KH4 i.e. subunit AurC | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrobenzoyl-CoA + malonyl-CoA + 4 (S)-methylmalonyl-CoA + 4 NADPH + 4 H+ | - |
Streptomyces thioluteus | demethylluteothin + 5 CO2 + 6 CoA + 4 NADP+ + 3 H2O | - |
? | |
4-nitrobenzoyl-CoA + malonyl-CoA + 4 (S)-methylmalonyl-CoA + 4 NADPH + 4 H+ | - |
Streptomyces thioluteus HKI-227 | demethylluteothin + 5 CO2 + 6 CoA + 4 NADP+ + 3 H2O | - |
? |
General Information | Comment | Organism |
---|---|---|
physiological function | model of the itinerative action of polyketide synthase. The ACP-bound polyketide intermediate is transferred back to the ketosynthase domain on the opposite polyketide synthase strand. the AurA N-terminus is not involved in the iteration process | Streptomyces thioluteus |