This polyketide synthase, characterized from the bacterium Streptomyces thioluteus, generates the backbone of the antibiotic aureothin. It is composed of 4 modules that total 18 domains and is encoded by three genes. The enzyme accepts the unusual starter unit 4-nitrobenzoyl-CoA and extends it by 4 molecules of (S)-methylmalonyl-CoA and a single molecule of malonyl-CoA. The first module (encoded by aurA) is used twice in an iterative fashion, so that the five Claisen condensation reactions are catalysed by only four modules. The iteration becomes possible by the transfer of the [acp]-bound polyketide intermediate back to the ketosynthase (KS) domain on the opposite polyketide synthase strand (polyketides are homodimeric).
The expected taxonomic range for this enzyme is: Streptomyces thioluteus
This polyketide synthase, characterized from the bacterium Streptomyces thioluteus, generates the backbone of the antibiotic aureothin. It is composed of 4 modules that total 18 domains and is encoded by three genes. The enzyme accepts the unusual starter unit 4-nitrobenzoyl-CoA and extends it by 4 molecules of (S)-methylmalonyl-CoA and a single molecule of malonyl-CoA. The first module (encoded by aurA) is used twice in an iterative fashion, so that the five Claisen condensation reactions are catalysed by only four modules. The iteration becomes possible by the transfer of the [acp]-bound polyketide intermediate back to the ketosynthase (KS) domain on the opposite polyketide synthase strand (polyketides are homodimeric).
the aureothin biosynthetic gene cluster is composed of 4 modules and is encoded by three genes. The enzyme extends 4-nitrobenzoyl-CoA by 4 molecules of (S)-methylmalonyl-CoA and a single molecule of malonyl-CoA. The first module (encoded by aurA) is used twice in an iterative fashion, so that the five Claisen condensation reactions are catalysed by only four modules
heterologous expression of an engineered AurAB fusion protein. Inactivation the ketosynthase or acyl carrier domains of module 4 clearly result in a shutdown of polyketide biosynthesis. Module 4 is required for the biosynthesis of polyketide production, and, despite the apparently inactive AT4 domain, an iterative use of the entirety of module 3 seems not to take place
comparison of Stretomyces thiolueus aureothin and Streptomyces orinoci neoaureothin gene clusters and enzymes. In both cases individual polyketide synthase modules are used in an iterative fashion
model of the itinerative action of polyketide synthase. The ACP-bound polyketide intermediate is transferred back to the ketosynthase domain on the opposite polyketide synthase strand. the AurA N-terminus is not involved in the iteration process
the aureothin biosynthetic gene cluster is composed of 4 modules and is encoded by three genes. The enzyme extends 4-nitrobenzoyl-CoA by 4 molecules of (S)-methylmalonyl-CoA and a single molecule of malonyl-CoA. The first module (encoded by aurA) is used twice in an iterative fashion, so that the five Claisen condensation reactions are catalysed by only four modules
heterologous expression of an engineered AurAB fusion protein. Inactivation the ketosynthase or acyl carrier domains of module 4 clearly result in a shutdown of polyketide biosynthesis. Module 4 is required for the biosynthesis of polyketide production, and, despite the apparently inactive AT4 domain, an iterative use of the entirety of module 3 seems not to take place
comparison of Stretomyces thiolueus aureothin and Streptomyces orinoci neoaureothin gene clusters and enzymes. In both cases individual polyketide synthase modules are used in an iterative fashion
model of the itinerative action of polyketide synthase. The ACP-bound polyketide intermediate is transferred back to the ketosynthase domain on the opposite polyketide synthase strand. the AurA N-terminus is not involved in the iteration process
engineering of the modular (type I) Aur polyketide synthase into a synthase that generates luteoreticulin unsing genes from a single biosynthesis gene cluster to design the artificial pathway
engineering of the modular (type I) Aur polyketide synthase into a synthase that generates luteoreticulin unsing genes from a single biosynthesis gene cluster to design the artificial pathway
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