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Literature summary for 2.3.1.28 extracted from

  • El-Gamal, B.; Temsah, S.; Olama, Z.; Mohamed, A.; El-Sayed, M.
    Purification and characterization of chloramphenicol acetyltransferase from Morganella morganii (2001), J. Biochem. Mol. Biol., 34, 415-420.
No PubMed abstract available
Search for more literature for 2.3.1.28

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Morganella morganii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17500
-
 1 * 19500 + 1 * 19000 + 1 * 18000 + 1 * 17500, SDS-PAGE Morganella morganii
18000
-
 1 * 19500 + 1 * 19000 + 1 * 18000 + 1 * 17500, SDS-PAGE Morganella morganii
19000
-
 1 * 19500 + 1 * 19000 + 1 * 18000 + 1 * 17500, SDS-PAGE Morganella morganii
19500
-
 1 * 19500 + 1 * 19000 + 1 * 18000 + 1 * 17500, SDS-PAGE Morganella morganii
78000
-
 gel filtration Morganella morganii

Organism

Organism UniProt Comment Textmining
Morganella morganii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Morganella morganii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Morganella morganii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + chloramphenicol
-
 Morganella morganii CoA + chloramphenicol 3-acetate
-
 ?

Subunits

Subunits Comment Organism
tetramer 1 * 19500 + 1 * 19000 + 1 * 18000 + 1 * 17500, SDS-PAGE Morganella morganii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Morganella morganii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 45 30°C: about 20% of maximal activity, 45°C: about 55% of maximal activity Morganella morganii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Morganella morganii

pH Range

pH Minimum pH Maximum Comment Organism
5 9 about 50% of maximal activity at pH 5.0 and 9.0 Morganella morganii