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Information on EC 2.3.1.28 - chloramphenicol O-acetyltransferase
for references in articles please use BRENDA:EC2.3.1.28
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EC Tree 2 Transferases
2.3 Acyltransferases
2.3.1 Transferring groups other than aminoacyl groups
2.3.1.28 chloramphenicol O-acetyltransferase
The enzyme appears in viruses and cellular organisms
- 2.3.1.28
-
5'-flanking
-
cotransfection
-
cis-acting
- tata
-
transactivation
- sp1
-
footprint
-
simian
- thymidine
- hepatoma
- immunodeficiency
- camp
- glucocorticoid
- dnase
- dexamethasone
-
phorbol
-
herpes
-
trans-acting
- simplex
- viruses
- sarcoma
- adenovirus
- c-fos
- nuclease
-
ccaat
-
promoterless
- cytomegalovirus
-
electroporation
-
nf-kappa
-
jurkat
-
run-on
-
dna-protein
-
immediate-early
- e1a
-
5'-deletion
-
gc-rich
- 12-o-tetradecanoylphorbol-13-acetate
-
5\'-upstream
-
camp-responsive
-
supershifted
-
polyhedrosis
-
mobility-shift
-
estrogen-responsive
-
subgenomic
- htlv-i
-
sp1-binding
-
glucocorticoid-responsive
-
basepairs
-
moloney
- analysis
- molecular biology
- medicine
- proviruses
- biotechnology
- synthesis
- pharmacology
showSynonyms
chloramphenicol acetyltransferase, cat-86, cat i, cat ii, chloramphenicol transacetylase, cat iii, acetyl-coa:chloramphenicol 3-o-acetyltransferase, chloramphenicol o-acetyltransferase, chloramphenicol acetylase, chloramphenicol acetyltransferase b2, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acetyltransferase, chloramphenicol
-
-
-
-
CAT
CAT I
-
-
-
-
CAT II
CAT III
-
-
-
-
CATI
chloramphenicol acetylase
-
-
-
-
chloramphenicol acetyltransferase
chloramphenicol transacetylase
-
-
-
-
CAT
-
-
-
-
chloramphenicol acetyltransferase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + chloramphenicol = CoA + chloramphenicol 3-acetate
ternary complex mechanism with a rapid equilibrium and essentially random order of addition of substrates
-
acetyl-CoA + chloramphenicol = CoA + chloramphenicol 3-acetate
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Acyl group transfer
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:chloramphenicol 3-O-acetyltransferase
-
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CAS REGISTRY NUMBER
COMMENTARY
9040-07-7
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2 acetyl-CoA + chloramphenicol
2 CoA + chloramphenicol 1,3-diacetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + 2-phenylethanol
CoA + 2-phenylethyl acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + benzyl alcohol
CoA + benzyl acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + isobutanol
CoA + isobutyl acetate
-
Substrates: -
Products: -
Products: -
?
chloramphenicol + acetyl-CoA
chloramphenicol 3-acetate + CoA
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: inducible enzyme
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: inducible enzyme
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
486712, 486713, 486714, 486717, 486719, 486720, 486723, 486725, 486730, 486731, 486733, 486736, 486737, 486738, 685590, 735976
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: in forward reaction formation of a ternary complex by a rapid-equilibrium mechanism, in reverse reaction rapid-equilibrium mechanism with random addition of substrates
Products: -
Products: -
r
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: catabolite repression of CAT synthesis is mediated by a mechanism involving cyclic adenosine 5'-monophosphate
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: inactivates chloramphenicol
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: enzymatic inactivation of chloramphenicol
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: all known R factors carrying the CAT gene in enteric bacteria mediate constitutive synthesis of the enzyme
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: in forward reaction formation of a ternary complex by a rapid-equilibrium mechanism, in reverse reaction rapid-equilibrium mechanism with random addition of substrates
Products: -
Products: -
r
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: acetylates only the biologically active D-threo stereoisomer
Products: + chloramphenicol 1,3-diacetate
Products: + chloramphenicol 1,3-diacetate
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: inducible enzyme
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: inactivates chloramphenicol
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: acetylates only the biologically active D-threo stereoisomer
Products: + chloramphenicol 1,3-diacetate
Products: + chloramphenicol 1,3-diacetate
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: inducible enzyme
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: inactivates chloramphenicol
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: inducible enzyme
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: inducible enzyme
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol 1-acetate
CoA + chloramphenicol 1,3-diacetate
-
Substrates: the enzyme acetylates specifically at the 3-hydroxy position. The diacetylation is possible only because of non-enzymatic interconversion of chloramphenical 3-acetate to chloramphenicol 1-acetate at higher pH values
Products: -
Products: -
?
acetyl-CoA + chloramphenicol 1-acetate
CoA + chloramphenicol 1,3-diacetate
-
Substrates: no activity
Products: -
Products: -
?
acetyl-CoA + chloramphenicol 1-acetate
CoA + chloramphenicol 1,3-diacetate
-
Substrates: no activity
Products: -
Products: -
?
acetyl-CoA + D-threo-1-p-nitrophenyl-2-bromoacetamido-1,3-propanediol
CoA + ?
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + D-threo-1-p-nitrophenyl-2-bromoacetamido-1,3-propanediol
CoA + ?
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + D-threo-1-p-nitrophenyl-2-bromoacetamido-1,3-propanediol
CoA + ?
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + D-threo-1-phenyl-2-dichloroacetamido-1,3-propanediol
CoA + ?
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + D-threo-1-phenyl-2-dichloroacetamido-1,3-propanediol
CoA + ?
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + D-threo-1-phenyl-2-dichloroacetamido-1,3-propanediol
CoA + ?
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: a CAP derivative on sulfidic monolayers on gold chips can still serve as a substrate for the enzyme
Products: -
Products: -
?
additional information
?
-
-
Substrates: poor substrates: butanol, propanol, ethanol, isopropanol. Specific ester production titers and rates are higher for aromatic alcohols than linear, short-chain alcohols
Products: -
Products: -
-
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
chloramphenicol + acetyl-CoA
chloramphenicol 3-acetate + CoA
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: inducible enzyme
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: inducible enzyme
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: catabolite repression of CAT synthesis is mediated by a mechanism involving cyclic adenosine 5'-monophosphate
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: inactivates chloramphenicol
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: enzymatic inactivation of chloramphenicol
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: all known R factors carrying the CAT gene in enteric bacteria mediate constitutive synthesis of the enzyme
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: inducible enzyme
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: inactivates chloramphenicol
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: inducible enzyme
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: inactivates chloramphenicol
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: inducible enzyme
Products: -
Products: -
?
acetyl-CoA + chloramphenicol
CoA + chloramphenicol 3-acetate
-
Substrates: inducible enzyme
Products: -
Products: -
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
HgCl2
-
at 0.1 M and 0.3 mM, no influence on the activity of the pSCS6-encoded enzyme. The activity of the pSCS7-encoded enzyme decreases to 10-15%. At 1.0 mM HgCl2, the pSCS6-encoded enzyme retains 50% of ist original activity, complete inactivation of the enzyme from pSCS7-encoded enzyme
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Crystal violet
-
strong, competitive for chloramphenicol, non-competitive for acetyl-CoA
ethyl violet
-
strong, competitive for chloramphenicol, non-competitive for acetyl-CoA
heme
identification and prediction of a His-based heme-regulatory motif. Heme interacts with chloroamphenicol acetyltransferase and displays an inhibitory effect on the protein activity
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.812