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Literature summary for 2.3.1.253 extracted from

  • Zhang, R.; Liu, W.; Cao, Y.; Xu, X.; Xian, M.; Liu, H.
    An in vitro synthetic biosystem based on acetate for production of phloroglucinol (2017), BMC Biotechnol., 17, 66 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.253

Application

Application Comment Organism
synthesis the enzyme can be used for production of important industrial chemicals from acetate in in vitro systems Pseudomonas fluorescens

Cloned(Commentary)

Cloned (Comment) Organism
codon-optimized gene phlD, functional recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), method optimization Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 malonyl-CoA Pseudomonas fluorescens
-
 phloroglucinol + 3 CO2 + 3 CoA
-
 ?
3 malonyl-CoA Pseudomonas fluorescens Pf-5
-
 phloroglucinol + 3 CO2 + 3 CoA
-
 ?
3 malonyl-CoA Pseudomonas fluorescens ATCC BAA-477
-
 phloroglucinol + 3 CO2 + 3 CoA
-
 ?
3 malonyl-CoA Pseudomonas fluorescens NRRL B-23932
-
 phloroglucinol + 3 CO2 + 3 CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens Q4K418
-
-
Pseudomonas fluorescens ATCC BAA-477 Q4K418
-
-
Pseudomonas fluorescens NRRL B-23932 Q4K418
-
-
Pseudomonas fluorescens Pf-5 Q4K418
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.03
-
 purified recombinant His-tagged enzyme, pH 7.0, 37°C Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 malonyl-CoA
-
 Pseudomonas fluorescens phloroglucinol + 3 CO2 + 3 CoA
-
 ?
3 malonyl-CoA
-
 Pseudomonas fluorescens Pf-5 phloroglucinol + 3 CO2 + 3 CoA
-
 ?
3 malonyl-CoA
-
 Pseudomonas fluorescens ATCC BAA-477 phloroglucinol + 3 CO2 + 3 CoA
-
 ?
3 malonyl-CoA
-
 Pseudomonas fluorescens NRRL B-23932 phloroglucinol + 3 CO2 + 3 CoA
-
 ?
additional information acetate is successfully transformed into phloroglucinol by the combined activity of recombinant acetyl-CoA carboxylase (ACC) from Acinetobacter calcoaceticus strain CGMCC 1.6186 and recombinant phloroglucinol synthase (PhlD) from Pseudomonas fluorescens strain Pf-5 in an in vitro system, synthetic pathway of phloroglucinol and in vitro synthesis of phloroglucinol from acetate, method optimization, overview Pseudomonas fluorescens ?
-
-
additional information acetate is successfully transformed into phloroglucinol by the combined activity of recombinant acetyl-CoA carboxylase (ACC) from Acinetobacter calcoaceticus strain CGMCC 1.6186 and recombinant phloroglucinol synthase (PhlD) from Pseudomonas fluorescens strain Pf-5 in an in vitro system, synthetic pathway of phloroglucinol and in vitro synthesis of phloroglucinol from acetate, method optimization, overview Pseudomonas fluorescens Pf-5 ?
-
-
additional information acetate is successfully transformed into phloroglucinol by the combined activity of recombinant acetyl-CoA carboxylase (ACC) from Acinetobacter calcoaceticus strain CGMCC 1.6186 and recombinant phloroglucinol synthase (PhlD) from Pseudomonas fluorescens strain Pf-5 in an in vitro system, synthetic pathway of phloroglucinol and in vitro synthesis of phloroglucinol from acetate, method optimization, overview Pseudomonas fluorescens ATCC BAA-477 ?
-
-
additional information acetate is successfully transformed into phloroglucinol by the combined activity of recombinant acetyl-CoA carboxylase (ACC) from Acinetobacter calcoaceticus strain CGMCC 1.6186 and recombinant phloroglucinol synthase (PhlD) from Pseudomonas fluorescens strain Pf-5 in an in vitro system, synthetic pathway of phloroglucinol and in vitro synthesis of phloroglucinol from acetate, method optimization, overview Pseudomonas fluorescens NRRL B-23932 ?
-
-

Synonyms

Synonyms Comment Organism
PhlD
-
 Pseudomonas fluorescens
type III polyketide synthase UniProt Pseudomonas fluorescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pseudomonas fluorescens