BRENDA - Enzyme Database show
show all sequences of 2.3.1.199

Enrichment of carotenoids in flaxseed (Linum usitatissimum) by metabolic engineering with introduction of bacterial phytoene synthase gene crtB

Fujisawa, M.; Watanabe, M.; Choi, S.K.; Teramoto, M.; Ohyama, K.; Misawa, N.; J. Biosci. Bioeng. 105, 636-641 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Arabidopsis thaliana and Brassica carinata
Crambe hispanica subsp. abyssinica
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56400
-
calculated from amino acid sequence
Crambe hispanica subsp. abyssinica
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Crambe hispanica subsp. abyssinica
Q32VC2
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
eicosenoyl-CoA + malonyl-CoA
22:1DELTA13
704734
Crambe hispanica subsp. abyssinica
?
-
-
-
?
erucoyl-CoA + malonyl-CoA
20:1DELTA11
704734
Crambe hispanica subsp. abyssinica
?
-
-
-
?
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Crambe hispanica subsp. abyssinica
calculated from amino acid sequence
-
9.3
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Arabidopsis thaliana and Brassica carinata
Crambe hispanica subsp. abyssinica
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56400
-
calculated from amino acid sequence
Crambe hispanica subsp. abyssinica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
eicosenoyl-CoA + malonyl-CoA
22:1DELTA13
704734
Crambe hispanica subsp. abyssinica
?
-
-
-
?
erucoyl-CoA + malonyl-CoA
20:1DELTA11
704734
Crambe hispanica subsp. abyssinica
?
-
-
-
?
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Crambe hispanica subsp. abyssinica
calculated from amino acid sequence
-
9.3
General Information
General Information
Commentary
Organism
physiological function
the enzyme has strong capability for improving the erucic acid content
Crambe hispanica subsp. abyssinica
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme has strong capability for improving the erucic acid content
Crambe hispanica subsp. abyssinica
Other publictions for EC 2.3.1.199
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735905
Purdy
Fatty acid elongase 7 catalyze ...
Homo sapiens
Cell Rep.
10
1375-1385
2015
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1
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1
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737030
Haslam
ECERIFERUM2-LIKE proteins have ...
Arabidopsis thaliana
Plant Physiol.
167
682-692
2015
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1
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1
2
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737032
Yamauchi
Ethylene biosynthesis is promo ...
Oryza sativa
Plant Physiol.
169
180-193
2015
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1
1
1
1
-
-
744399
Morais
Docosahexaenoic acid biosynth ...
Solea senegalensis
Biochim. Biophys. Acta
1851
588-597
2015
-
-
-
-
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1
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4
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-
4
-
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-
-
-
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-
-
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-
1
1
1
1
-
-
737127
Naganuma
Two modes of regulation of the ...
Homo sapiens
PLoS ONE
9
e101823
2014
2
-
1
-
-
-
-
1
1
1
2
1
-
1
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1
1
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1
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3
1
1
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1
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2
-
1
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1
1
1
2
1
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-
1
1
1
-
-
-
3
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-
736796
Sassa
Impaired epidermal permeabilit ...
Mus musculus
Mol. Cell. Biol.
33
2787-2796
2013
-
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1
1
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736971
Tsuda
ONION2 fatty acid elongase is ...
Oryza sativa
Plant Cell Physiol.
54
209-217
2013
-
-
-
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5
-
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1
1
-
-
-
720637
Tresch
Inhibition of saturated very-l ...
Arabidopsis thaliana, Lemna aequinoctialis
Phytochemistry
76
162-171
2012
-
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1
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-
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6
-
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6
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6
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6
-
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1
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6
6
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6
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720673
Reina-Pinto
Misexpression of fatty acid el ...
Arabidopsis thaliana
Plant Cell
21
1252-1272
2009
-
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-
-
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1
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4
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3
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1
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1
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3
-
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1
-
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-
-
-
-
-
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2
2
-
-
-
720703
Lee
Two Arabidopsis 3-ketoacyl CoA ...
Arabidopsis thaliana
Plant J.
60
462-475
2009
-
-
-
-
-
-
-
-
-
-
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2
-
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9
-
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1
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9
-
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1
-
-
-
-
-
-
-
-
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1
2
2
1
-
-
690742
Blacklock
A fatty acid elongase ELO with ...
Dictyostelium discoideum, Dictyostelium discoideum AX3
Biochem. Biophys. Res. Commun.
374
226-230
2008
-
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1
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4
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2
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704734
Fujisawa
Enrichment of carotenoids in f ...
Crambe hispanica subsp. abyssinica
J. Biosci. Bioeng.
105
636-641
2008
-
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1
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1
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6
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2
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1
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1
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2
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1
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1
1
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-
720125
Qin
Genetic and biochemical studie ...
Gossypium hirsutum
J. Exp. Bot.
58
473-481
2007
-
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1
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1
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1
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4
-
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2
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2
1
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1
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2
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2
1
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1
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1
-
-
718730
Blacklock
Substrate specificity of Arabi ...
Arabidopsis thaliana
Biochem. Biophys. Res. Commun.
346
583-590
2006
-
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1
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4
-
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1
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5
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1
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1
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5
-
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719484
Katavic
Gaining insight into the role ...
Brassica napus
FEBS Lett.
562
118-124
2004
-
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1
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9
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3
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3
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1
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9
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3
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719400
Ghanevati
Engineering and mechanistic st ...
Arabidopsis thaliana
Eur. J. Biochem.
269
3531-3539
2002
-
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1
-
6
-
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1
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1
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3
-
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1
-
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1
-
-
7
1
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1
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1
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1
1
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6
-
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1
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1
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1
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1
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7
1
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1
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718807
Kunst
Expression of the wax-specific ...
Arabidopsis thaliana
Biochem. Soc. Trans.
28
651-654
2000
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1
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4
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6
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1
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6
-
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1
1
1
1
-
-
720670
Fiebig
Alterations in CER6, a gene id ...
Arabidopsis thaliana
Plant Cell
12
2001-2008
2000
-
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4
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2
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2
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1
1
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720669
Millar
CUT1, an Arabidopsis gene requ ...
Arabidopsis thaliana
Plant Cell
11
825-838
1999
-
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3
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3
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3
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2
2
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720698
Todd
KCS1 encodes a fatty acid elon ...
Arabidopsis thaliana
Plant J.
17
119-130
1999
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1
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1
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3
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6
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2
1
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1
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1
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6
-
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2
1
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2
2
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487614
Dittrich
Fatty acid elongation in yeast ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae X2180-1A
Eur. J. Biochem.
252
477-485
1998
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-
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5
1
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1
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6
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9
1
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5
1
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9
1
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-
719791
Oh
ELO2 and ELO3, homologues of t ...
Saccharomyces cerevisiae
J. Biol. Chem.
272
17376-17384
1997
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1
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2
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1
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2
2
-
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-
720697
Millar
Very-long-chain fatty acid bio ...
Arabidopsis thaliana
Plant J.
12
121-131
1997
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1
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1
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5
-
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1
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1
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1
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1
1
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-
719787
Toke
Isolation and characterization ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae W3031B
J. Biol. Chem.
271
18413-18422
1996
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1
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1
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