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Literature summary for 2.3.1.183 extracted from

  • Kita, Y.; Hanafy, M.; Deguchi, M.; Hasegawa, H.; Terakawa, T.; Kitamura, K.; Ishimoto, M.
    Generation and characterization of herbicide-resistant soybean plants expressing novel phosphinothricin N-acetyltransferase genes (2009), Breed. Sci., 59, 245-251.
No PubMed abstract available
Search for more literature for 2.3.1.183

Application

Application Comment Organism
agriculture creation of herbicide resistant plants Nocardia sp.
agriculture creation of herbicide resistant plants Streptomyces sp.
biotechnology creation of herbicide resistant plants Nocardia sp.
biotechnology creation of herbicide resistant plants Streptomyces sp.

Cloned(Commentary)

Cloned (Comment) Organism
expressed in soybean after transformation with Agrobacterium tumefaciens Nocardia sp.
expressed in soybean after transformation with Agrobacterium tumefaciens Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 SDS-PAGE Nocardia sp.
21000
-
 SDS-PAGE Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Nocardia sp.
-
 soil isolate
-
Streptomyces sp.
-
 soil isolate, DDBJ no. DD355820
-
Streptomyces sp. AB3534
-
 soil isolate, DDBJ no. DD355820
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nocardia sp.
-
 Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0177
-
 at 30°C, enzyme activity in crude extract of the transgenic soybean line h-7, in wild-type plants no activity is detectable Streptomyces sp.
0.0221
-
 at 30°C, enzyme activity in crude extract of the transgenic soybean line m-12, in wild-type plants no activity is detectable Nocardia sp.
0.0357
-
 at 30°C, enzyme activity in crude extract of the transgenic soybean line h-9, in wild-type plants no activity is detectable Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphinothricin + acetyl-CoA
-
 Nocardia sp. L-N-acetylphosphinothricin + CoA
-
 ?
phosphinothricin + acetyl-CoA
-
 Streptomyces sp. L-N-acetylphosphinothricin + CoA
-
 ?
phosphinothricin + acetyl-CoA
-
 Streptomyces sp. AB3534 L-N-acetylphosphinothricin + CoA
-
 ?

Synonyms

Synonyms Comment Organism
hpat
-
 Streptomyces sp.
MAT
-
 Nocardia sp.
methionine sulfone N-acetyltransferase
-
 Nocardia sp.
phosphinothricin-N-acetyltransferase
-
 Nocardia sp.
phosphinothricin-N-acetyltransferase
-
 Streptomyces sp.