Literature summary for 2.3.1.183 extracted from

Madduri, K.M.; Snodderley, E.M.
Expression of phosphinothricin N-acetyltransferase in Escherichia coli and Pseudomonas fluorescens: Influence of mRNA secondary structure, host, and other physiological conditions (2007), Protein Expr. Purif., 55, 352-360.

Search for more literature for 2.3.1.183

Application

Application	Comment	Organism
agriculture	since the presence of the PAT gene confers resistance to the host harboring it, this gene has been used extensively as a selectable marker for the transformation of a wide range of plants	Streptomyces viridochromogenes

Cloned(Commentary)

Cloned (Comment)	Organism
a linear template for in vitro transcription and translation is created, the PAT gene is cloned into the vector pCR2.1TOPO, verified and subcloned into pET280 and pDOW1169 for expression in Escherichia coli or Pseudomonas fluorescens, respectively	Streptomyces viridochromogenes
overexpression in Escherichia coli Bl21(DE3) and Pseudomonas fluorescens strain DC454 requiring modification of the 5' end of the pat ORF, in vitro transcription and translation, optimization of the expression method, Pseudomonas fluorescens is the better host, the secondary structures in the 5' coding region of pat gene influences the expression efficiency, overview	Streptomyces viridochromogenes

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
21000	-	determined by SDS-PAGE	Streptomyces viridochromogenes

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetyl-CoA + phosphinothricin	Streptomyces viridochromogenes	-	CoA + N-acetylphosphinothricin	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces viridochromogenes	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
for the purification of PAT from Pseudomonas fluorescens ammonium sulfate precipitation, a Source 15 Phenyl XK hydrophobic interaction column, and a Superdex 75 XK gel filtration column are used	Streptomyces viridochromogenes
recombinant enzyme from Pseudomonas fluorescens to homogeneity by ammonium sulfate fractionation, hydrophobic interaction chromatography, and gel filtration	Streptomyces viridochromogenes

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + phosphinothricin	-	Streptomyces viridochromogenes	CoA + N-acetylphosphinothricin	-	?

Synonyms

Synonyms	Comment	Organism
Pat	-	Streptomyces viridochromogenes
phosphinothricin N-acetyltransferase	-	Streptomyces viridochromogenes

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Release 2023.1 (January 2023)