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Literature summary for 2.3.1.183 extracted from
- Transgenic plants containing the phosphinothricin-N-acetyltransferase gene metabolize the herbicide L-phosphinothricin (glufosinate) differently from untransformed plants (1992), Planta, 187, 142-151.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pat, functional expression in transgenic Nicotiana tabacum and Daucus carota plant leaves using the Agrobacterium tumefaciens strain LBA4404 transfection of leaves and direct gene transfer to protoplasts, respectively, subcloning in Escherichia coli strain S 17. I, the transgenic plants are resistant to L-phosphinothricin | Streptomyces viridochromogenes |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | transgenic Nicotiana tabacum and Daucus carota plants expressing the enzyme are resistant to L-phosphinothricin, L-phosphinothricin metabolism including decarboxylation and CO2 release in wild-type plants and transgenic plants, overview | Streptomyces viridochromogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + L-phosphinothricin | Streptomyces viridochromogenes | the enzyme confers resistance to the herbicide phosphinothricin, which is deaminated to form its corresponding oxo acid 4-methylphosphinico-2-oxo-butanoic acid, which subsequently is decarboxylated to form 3-methylphosphinico-propanoic acid, a stable compound in the plant | CoA + L-N-acetylphosphinothricin | - |
? |  |
| acetyl-CoA + L-phosphinothricin | Streptomyces viridochromogenes Tue494 | the enzyme confers resistance to the herbicide phosphinothricin, which is deaminated to form its corresponding oxo acid 4-methylphosphinico-2-oxo-butanoic acid, which subsequently is decarboxylated to form 3-methylphosphinico-propanoic acid, a stable compound in the plant | CoA + L-N-acetylphosphinothricin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces viridochromogenes | - |
gene pat | - |
| Streptomyces viridochromogenes Tue494 | - |
gene pat | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Streptomyces viridochromogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + L-phosphinothricin | the enzyme confers resistance to the herbicide phosphinothricin, which is deaminated to form its corresponding oxo acid 4-methylphosphinico-2-oxo-butanoic acid, which subsequently is decarboxylated to form 3-methylphosphinico-propanoic acid, a stable compound in the plant | Streptomyces viridochromogenes | CoA + L-N-acetylphosphinothricin | - |
? | |
| acetyl-CoA + L-phosphinothricin | analysis of degradation of L-phosphinothricin 14C-labeled specifically at different C-atoms, overview | Streptomyces viridochromogenes | CoA + L-N-acetylphosphinothricin | product determination and identification | ? | |
| acetyl-CoA + L-phosphinothricin | the enzyme confers resistance to the herbicide phosphinothricin, which is deaminated to form its corresponding oxo acid 4-methylphosphinico-2-oxo-butanoic acid, which subsequently is decarboxylated to form 3-methylphosphinico-propanoic acid, a stable compound in the plant | Streptomyces viridochromogenes Tue494 | CoA + L-N-acetylphosphinothricin | - |
? | |
| acetyl-CoA + L-phosphinothricin | analysis of degradation of L-phosphinothricin 14C-labeled specifically at different C-atoms, overview | Streptomyces viridochromogenes Tue494 | CoA + L-N-acetylphosphinothricin | product determination and identification | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pat | - |
Streptomyces viridochromogenes |
| phosphinothricin-N-acetyltransferase | - |
Streptomyces viridochromogenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Streptomyces viridochromogenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Streptomyces viridochromogenes |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Streptomyces viridochromogenes | |
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