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Literature summary for 2.3.1.183 extracted from
- Application of immunoaffinity column as cleanup tool for an enzyme linked immunosorbent assay of phosphinothricin-N-acetyltransferase detection in genetically modified maize and rape (2005), J. Agric. Food Chem., 53, 4315-4321.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene bar, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), expression in transgenic maize and rape plant leaves | Streptomyces hygroscopicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of transgenic Zea mays and Brassica sp. plants expressing the bar gene, expression analysis, overview | Streptomyces hygroscopicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + L-phosphinothricin | Streptomyces hygroscopicus | the enzyme is responsible for resistance to L-phosphinothricin and bialaphos | CoA + L-N-acetylphosphinothricin | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces hygroscopicus | P16426 | gene bar; gene bar | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant enzyme from transgenic maize and rape plants by immunoaffinity chromatography, method development, overview | Streptomyces hygroscopicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + L-phosphinothricin | - |
Streptomyces hygroscopicus | CoA + L-N-acetylphosphinothricin | - |
? | |
| acetyl-CoA + L-phosphinothricin | the enzyme is responsible for resistance to L-phosphinothricin and bialaphos | Streptomyces hygroscopicus | CoA + L-N-acetylphosphinothricin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pat | - |
Streptomyces hygroscopicus |
| phosphinothricin-N-acetyltransferase | - |
Streptomyces hygroscopicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Streptomyces hygroscopicus | |
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Release 2023.1 (January 2023)
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