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Literature summary for 2.3.1.183 extracted from

  • Bartsch, K.; Tebbe, C.C.
    Initial steps in the degradation of phosphinothricin (glufosinate) by soil bacteria (1989), Appl. Environ. Microbiol., 55, 711-716.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.183

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme activity is inducible 10fold by L-phosphinothricin Alcaligenes faecalis

Inhibitors

Inhibitors Comment Organism Structure
aminooxyacetic acid
-
 Rhodococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-phosphinothricin Alcaligenes faecalis stereospecific reaction, no activity with D-isomer CoA + L-N-acetylphosphinothricin
-
 ?
acetyl-CoA + L-phosphinothricin Rhodococcus sp. stereospecific reaction, no activity with D-isomer CoA + L-N-acetylphosphinothricin
-
 ?
acetyl-CoA + L-phosphinothricin Sphingomonas paucimobilis stereospecific reaction, no activity with D-isomer CoA + L-N-acetylphosphinothricin
-
 ?
acetyl-CoA + L-phosphinothricin Rhodococcus sp. DX-35 stereospecific reaction, no activity with D-isomer CoA + L-N-acetylphosphinothricin
-
 ?
acetyl-CoA + L-phosphinothricin Sphingomonas paucimobilis FX-90 stereospecific reaction, no activity with D-isomer CoA + L-N-acetylphosphinothricin
-
 ?

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-
Rhodococcus sp.
-
-
-
Rhodococcus sp. DX-35
-
-
-
Sphingomonas paucimobilis
-
-
-
Sphingomonas paucimobilis FX-90
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information the organism cannot utilize L-phosphinothricin as a sole source of nitrogen Alcaligenes faecalis
-
additional information the organism is able to utilize L-phosphinothricin as a sole source of nitrogen Rhodococcus sp.
-
additional information the organism is able to utilize L-phosphinothricin as a sole source of nitrogen Sphingomonas paucimobilis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
 induced activity, cell extract Alcaligenes faecalis
0.0212
-
 strain DX-35, cell extract Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-phosphinothricin stereospecific reaction, no activity with D-isomer Alcaligenes faecalis CoA + L-N-acetylphosphinothricin
-
 ?
acetyl-CoA + L-phosphinothricin stereospecific reaction, no activity with D-isomer Rhodococcus sp. CoA + L-N-acetylphosphinothricin
-
 ?
acetyl-CoA + L-phosphinothricin stereospecific reaction, no activity with D-isomer Sphingomonas paucimobilis CoA + L-N-acetylphosphinothricin
-
 ?
acetyl-CoA + L-phosphinothricin i.e. L-homoalanin-4-yl(methyl)phosphinic acid, the active ingredient of the herbicide BASTA, stereospecific reaction, no activity with D-isomer Alcaligenes faecalis CoA + L-N-acetylphosphinothricin
-
 ?
acetyl-CoA + L-phosphinothricin i.e. L-homoalanin-4-yl(methyl)phosphinic acid, the active ingredient of the herbicide BASTA, stereospecific reaction, no activity with D-isomer Rhodococcus sp. CoA + L-N-acetylphosphinothricin
-
 ?
acetyl-CoA + L-phosphinothricin i.e. L-homoalanin-4-yl(methyl)phosphinic acid, the active ingredient of the herbicide BASTA, stereospecific reaction, no activity with D-isomer Sphingomonas paucimobilis CoA + L-N-acetylphosphinothricin
-
 ?
acetyl-CoA + L-phosphinothricin stereospecific reaction, no activity with D-isomer Rhodococcus sp. DX-35 CoA + L-N-acetylphosphinothricin
-
 ?
acetyl-CoA + L-phosphinothricin i.e. L-homoalanin-4-yl(methyl)phosphinic acid, the active ingredient of the herbicide BASTA, stereospecific reaction, no activity with D-isomer Rhodococcus sp. DX-35 CoA + L-N-acetylphosphinothricin
-
 ?
acetyl-CoA + L-phosphinothricin stereospecific reaction, no activity with D-isomer Sphingomonas paucimobilis FX-90 CoA + L-N-acetylphosphinothricin
-
 ?
acetyl-CoA + L-phosphinothricin i.e. L-homoalanin-4-yl(methyl)phosphinic acid, the active ingredient of the herbicide BASTA, stereospecific reaction, no activity with D-isomer Sphingomonas paucimobilis FX-90 CoA + L-N-acetylphosphinothricin
-
 ?

Synonyms

Synonyms Comment Organism
L-PPT N-acetyltransferase
-
 Alcaligenes faecalis
L-PPT N-acetyltransferase
-
 Rhodococcus sp.
L-PPT N-acetyltransferase
-
 Sphingomonas paucimobilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Alcaligenes faecalis
30
-
 assay at Rhodococcus sp.
30
-
 assay at Sphingomonas paucimobilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 assay at Alcaligenes faecalis
8.2
-
 assay at Rhodococcus sp.
8.2
-
 assay at Sphingomonas paucimobilis

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Alcaligenes faecalis
acetyl-CoA
-
 Rhodococcus sp.
acetyl-CoA
-
 Sphingomonas paucimobilis