BRENDA - Enzyme Database
show all sequences of 2.3.1.153

Anthocyanin 5-aromatic acyltransferase from Gentiana triflora

Fujiwara, H.; Tanaka, Y.; Fukui, Y.; Nakao, M.; Ashikari, T.; Kusumi, T.; Eur. J. Biochem. 249, 45-51 (1997)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
PCMB
90% inhibition at 0.1 mM, complete inhibition at 1 mM
Gentiana triflora
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.065
-
caffeoyl-CoA
with delphinidin 3,5-diglucoside as cosubstrate
Gentiana triflora
0.087
-
cyanidin 3,5-diglucoside
with caffeoyl-CoA as cosubstrate
Gentiana triflora
0.15
-
delphinidin 3,5-diglucoside
with caffeoyl-CoA as cosubstrate
Gentiana triflora
0.19
-
p-Coumaroyl-CoA
with delphinidin 3,5-diglucoside as cosubstrate
Gentiana triflora
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
strongly enhances activity
Gentiana triflora
Zn2+
moderately enhances activity
Gentiana triflora
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49000
-
gel filtration
Gentiana triflora
52000
-
1 * 52000, SDS-PAGE
Gentiana triflora
Organism
Organism
UniProt
Commentary
Textmining
Gentiana triflora
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Gentiana triflora
Source Tissue
Source Tissue
Commentary
Organism
Textmining
petal
blue
Gentiana triflora
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Gentiana triflora
Storage Stability
Storage Stability
Organism
-20°C, 20 mM Tris/HCl, pH 7.0, 0.01 mM (p-aminidinophenyl)methanesulfonyl fluoride, 1 mM DTT, stable for at least 2 years
Gentiana triflora
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
cyanidin-3,5-O-beta-D-diglucoside + 4-coumaroyl-CoA
-
16329
Gentiana triflora
CoA + cyanidin 3-O-beta-D-glucoside-5-O-(6-O-4-coumaroyl)-beta-D-glucoside
-
-
-
?
cyanidin-3,5-O-beta-D-diglucoside + caffeoyl-CoA
-
16329
Gentiana triflora
CoA + cyanidin 3-O-beta-D-glucoside-5-O-(6-O-caffeoyl)-beta-D-glucoside
-
-
-
?
delphinidin 3,5-di-O-beta-D-glucoside + 4-coumaroyl-CoA
-
16329
Gentiana triflora
CoA + delphinidin 3-O-beta-D-glucoside-5-O-(6-O-4-coumaroyl)-beta-D-glucoside
-
-
-
?
delphinidin 3,5-di-O-beta-D-glucoside + caffeoyl-CoA
-
16329
Gentiana triflora
CoA + delphinidin 3-O-beta-D-glucosyl-5-O-(6-O-caffeoyl-beta-D-glucoside)
-
16329
Gentiana triflora
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 52000, SDS-PAGE
Gentiana triflora
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
8.5
-
Gentiana triflora
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
10
active in this range
Gentiana triflora
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
PCMB
90% inhibition at 0.1 mM, complete inhibition at 1 mM
Gentiana triflora
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.065
-
caffeoyl-CoA
with delphinidin 3,5-diglucoside as cosubstrate
Gentiana triflora
0.087
-
cyanidin 3,5-diglucoside
with caffeoyl-CoA as cosubstrate
Gentiana triflora
0.15
-
delphinidin 3,5-diglucoside
with caffeoyl-CoA as cosubstrate
Gentiana triflora
0.19
-
p-Coumaroyl-CoA
with delphinidin 3,5-diglucoside as cosubstrate
Gentiana triflora
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
strongly enhances activity
Gentiana triflora
Zn2+
moderately enhances activity
Gentiana triflora
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49000
-
gel filtration
Gentiana triflora
52000
-
1 * 52000, SDS-PAGE
Gentiana triflora
Purification (Commentary) (protein specific)
Commentary
Organism
-
Gentiana triflora
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
petal
blue
Gentiana triflora
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Gentiana triflora
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 20 mM Tris/HCl, pH 7.0, 0.01 mM (p-aminidinophenyl)methanesulfonyl fluoride, 1 mM DTT, stable for at least 2 years
Gentiana triflora
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
cyanidin-3,5-O-beta-D-diglucoside + 4-coumaroyl-CoA
-
16329
Gentiana triflora
CoA + cyanidin 3-O-beta-D-glucoside-5-O-(6-O-4-coumaroyl)-beta-D-glucoside
-
-
-
?
cyanidin-3,5-O-beta-D-diglucoside + caffeoyl-CoA
-
16329
Gentiana triflora
CoA + cyanidin 3-O-beta-D-glucoside-5-O-(6-O-caffeoyl)-beta-D-glucoside
-
-
-
?
delphinidin 3,5-di-O-beta-D-glucoside + 4-coumaroyl-CoA
-
16329
Gentiana triflora
CoA + delphinidin 3-O-beta-D-glucoside-5-O-(6-O-4-coumaroyl)-beta-D-glucoside
-
-
-
?
delphinidin 3,5-di-O-beta-D-glucoside + caffeoyl-CoA
-
16329
Gentiana triflora
CoA + delphinidin 3-O-beta-D-glucosyl-5-O-(6-O-caffeoyl-beta-D-glucoside)
-
16329
Gentiana triflora
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 52000, SDS-PAGE
Gentiana triflora
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
8.5
-
Gentiana triflora
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
10
active in this range
Gentiana triflora
Other publictions for EC 2.3.1.153
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
758078
de Rosas
Loss of anthocyanins and modi ...
Vitis vinifera
Plant Sci.
258
137-145
2017
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
689603
Griesser
Redirection of flavonoid biosy ...
Gentiana scabra x Gentiana triflora, Gentiana triflora, Gentiana triflora Homoi, Gentiana scabra x Gentiana triflora Polano White, Gentiana triflora Maciry
Plant Physiol.
146
1528-1539
2008
-
-
2
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
10
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
686755
DAuria
Identification and characteriz ...
Arabidopsis thaliana
FEBS Lett.
581
872-878
2007
-
-
1
-
-
-
-
6
-
-
3
-
-
5
-
-
1
-
-
1
-
-
4
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
-
3
-
-
-
-
1
-
1
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
16329
Fujiwara
Anthocyanin 5-aromatic acyltra ...
Gentiana triflora
Eur. J. Biochem.
249
45-51
1997
-
-
-
-
-
-
1
4
-
2
2
-
-
3
-
-
1
-
-
1
1
1
4
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
2
2
-
-
-
-
1
-
1
1
1
4
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-