We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments Isolated from the plant Gentiana triflora. Transfers the hydroxycinnamoyl group only to the C-5 glucoside of anthocyanin. Caffeoyl-CoA, but not malonyl-CoA, can substitute as an acyl donor.
The enzyme appears in viruses and cellular organisms
Reaction Schemes
+
=
+
anthocyanidin 3-O-beta-D-glucoside 5-O-beta-D-(6-O-4-hydroxycinnamoylglucoside)
Synonyms
5AT, Acyltransferase, anthocyanin, Anthocyanidin 3,5-diglucoside 5-O-glucoside-6''-O-hydroxycinnamoyltransferase, anthocyanidin 5-O-glucoside-6''-O-malonyltransferase, anthocyanin 5-aromatic acyltransferase, Anthocyanin acyltransferase, Anthocyanin hydroxycinnamoyltransferase, Gentian 5AT, Hydroxycinnamoyl-CoA-anthocyanidin 3,5-diglucoside 5-O-glucoside-6'''-O-hydroxycinnamoyltransferase, Hydroxycinnamoyltransferase, anthocyanidin 3,5-diglucoside 5-O-glucoside-6''-O-,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Acyltransferase, anthocyanin
-
-
-
-
Anthocyanidin 3,5-diglucoside 5-O-glucoside-6''-O-hydroxycinnamoyltransferase
-
-
-
-
anthocyanidin 5-O-glucoside-6''-O-malonyltransferase
-
belongs to the BAHD acyltransferase malonyl CoA family
anthocyanin 5-aromatic acyltransferase
Anthocyanin acyltransferase
-
-
-
-
Anthocyanin hydroxycinnamoyltransferase
-
-
-
-
Hydroxycinnamoyl-CoA-anthocyanidin 3,5-diglucoside 5-O-glucoside-6'''-O-hydroxycinnamoyltransferase
-
-
-
-
Hydroxycinnamoyltransferase, anthocyanidin 3,5-diglucoside 5-O-glucoside-6''-O-
-
-
-
-
Hydroxycinnamoyltransferase, anthocyanin
-
-
-
-
5AT
-
-
anthocyanin 5-aromatic acyltransferase
-
At5MAT
anthocyanin 5-aromatic acyltransferase
-
-
anthocyanin 5-aromatic acyltransferase
-
-
-
anthocyanin 5-aromatic acyltransferase
-
anthocyanin 5-aromatic acyltransferase
-
-
anthocyanin 5-aromatic acyltransferase
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4-hydroxycinnamoyl-CoA + an anthocyanidin 3,5-di-O-beta-D-glucoside = CoA + anthocyanidin 3-O-beta-D-glucoside 5-O-beta-D-(6-O-4-hydroxycinnamoylglucoside)
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Acyl group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4-hydroxycinnamoyl-CoA:anthocyanidin 3,5-di-O-beta-D-glucoside 5-O-glucoside-6'''-O-4-hydroxycinnamoyltransferase
Isolated from the plant Gentiana triflora. Transfers the hydroxycinnamoyl group only to the C-5 glucoside of anthocyanin. Caffeoyl-CoA, but not malonyl-CoA, can substitute as an acyl donor.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-(3,4-dihydroxyphenyl)-3-[[6-O-[(2E)-3-[4-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoyl]-beta-D-glucopyranosyl-(1-6)-[2-O-[(2E)-3-(4-hydroxy-3,5-dimethoxyphenyl)prop-2-enoyl]-beta-D-xylopyranosyl-(1-2)]-beta-D-glucopyranosyl]oxy]-7-hydroxychromenium-5-yl beta-D-glucopyranoside + malonyl-CoA
5-[[6-O-(carboxyacetyl)-beta-D-glucopyranosyl]oxy]-2-(3,4-dihydroxyphenyl)-7-hydroxychromenium-3-yl 6-O-[(2E)-3-[4-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoyl]-beta-D-glucopyranosyl-(1-6)-[2-O-[(2E)-3-(4-hydroxy-3,5-dimethoxyphenyl)prop-2-enoyl]-beta-D-xylopyranosyl-(1-2)]-beta-D-glucopyranoside + CoA
-
cyanindin A10
-
-
?
2-(3,4-dihydroxyphenyl)-3-[[6-O-[(2E)-3-[4-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoyl]-beta-D-glucopyranosyl-(1-6)-[beta-D-xylopyranosyl-(1-2)]-beta-D-glucopyranosyl]oxy]-7-hydroxychromenium-5-yl beta-D-glucopyranoside + malonyl-CoA
5-[[6-O-(carboxyacetyl)-beta-D-glucopyranosyl]oxy]-2-(3,4-dihydroxyphenyl)-7-hydroxychromenium-3-yl 6-O-[(2E)-3-[4-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoyl]-beta-D-glucopyranosyl-(1-6)-[beta-D-xylopyranosyl-(1-2)]-beta-D-glucopyranoside + CoA
-
cyanindin A6, malonylation occurs exclusively on the 5-O-glucose
-
-
?
cyanidin-3,5-O-beta-D-diglucoside + 4-coumaroyl-CoA
CoA + cyanidin 3-O-beta-D-glucoside-5-O-(6-O-4-coumaroyl)-beta-D-glucoside
-
-
-
-
?
cyanidin-3,5-O-beta-D-diglucoside + caffeoyl-CoA
CoA + cyanidin 3-O-beta-D-glucoside-5-O-(6-O-caffeoyl)-beta-D-glucoside
-
-
-
-
?
cyanin + malonyl-CoA
5-[[6-O-(carboxyacetyl)-b-D-glucopyranosyl]oxy]-2-(3,4-dihydroxyphenyl)-7-hydroxychromenium-3-yl beta-D-glucopyranoside + CoA
-
-
-
-
?
delphinidin 3,5-di-O-beta-D-glucoside + 4-coumaroyl-CoA
CoA + delphinidin 3-O-beta-D-glucoside-5-O-(6-O-4-coumaroyl)-beta-D-glucoside
-
-
-
-
?
delphinidin 3,5-di-O-beta-D-glucoside + caffeoyl-CoA
CoA + delphinidin 3-O-beta-D-glucosyl-5-O-(6-O-caffeoyl-beta-D-glucoside)
-
-
-
?
malvin + malonyl-CoA
5-[[6-O-(carboxyacetyl)-beta-D-glucopyranosyl]oxy]-7-hydroxy-2-(4-hydroxy-3,5-dimethoxyphenyl)chromenium-3-yl beta-D-glucopyranoside + CoA
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Mn2+
-
strongly enhances activity
Zn2+
-
moderately enhances activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PCMB
-
90% inhibition at 0.1 mM, complete inhibition at 1 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0076
2-(3,4-dihydroxyphenyl)-3-[[6-O-[(2E)-3-[4-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoyl]-beta-D-glucopyranosyl-(1-6)-[2-O-[(2E)-3-(4-hydroxy-3,5-dimethoxyphenyl)prop-2-enoyl]-beta-D-xylopyranosyl-(1-2)]-beta-D-glucopyranosyl]oxy]-7-hydroxychromenium-5-yl beta-D-glucopyranoside
-
-
0.07
2-(3,4-dihydroxyphenyl)-3-[[6-O-[(2E)-3-[4-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoyl]-beta-D-glucopyranosyl-(1-6)-[beta-D-xylopyranosyl-(1-2)]-beta-D-glucopyranosyl]oxy]-7-hydroxychromenium-5-yl beta-D-glucopyranoside
-
-
0.065
caffeoyl-CoA
-
with delphinidin 3,5-diglucoside as cosubstrate
0.087
cyanidin 3,5-diglucoside
-
with caffeoyl-CoA as cosubstrate
0.15
delphinidin 3,5-diglucoside
-
with caffeoyl-CoA as cosubstrate
0.0021 - 0.0153
malonyl-CoA
0.19
p-Coumaroyl-CoA
-
with delphinidin 3,5-diglucoside as cosubstrate
0.0021
malonyl-CoA
-
2-(3,4-dihydroxyphenyl)-3-[[6-O-[(2E)-3-[4-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoyl]-beta-D-glucopyranosyl-(1-6)-[2-O-[(2E)-3-(4-hydroxy-3,5-dimethoxyphenyl)prop-2-enoyl]-beta-D-xylopyranosyl-(1-2)]-beta-D-glucopyranosyl]oxy]-7-hydroxychromenium-5-yl beta-D-glucopyranoside as cosubstrate
0.0153
malonyl-CoA
-
2-(3,4-dihydroxyphenyl)-3-[[6-O-[(2E)-3-[4-(beta-D-glucopyranosyloxy)phenyl]prop-2-enoyl]-beta-D-glucopyranosyl-(1-6)-[beta-D-xylopyranosyl-(1-2)]-beta-D-glucopyranosyl]oxy]-7-hydroxychromenium-5-yl beta-D-glucopyranoside as cosubstrate
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5.5 - 10
-
active in this range
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4-6-week-old rosette stage wild type, At3g29590, a mutant line incapable of producing malonylated anthocyanins and transgenic line harboring anRNAi silencing cassette for the enzyme
-
-
brenda
white flower cultivar
-
-
brenda
white flower cultivar
-
-
brenda
white flower cultivar
UniProt
brenda
blue flower cultivar
UniProt
brenda
-
UniProt
brenda
-
-
-
brenda
blue flower cultivar
UniProt
brenda
white flower cultivar
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
skin
brenda
-
bud stage and just before anthesis
brenda
-
bud stage and just before anthesis
-
brenda
bud stage and just before anthesis
brenda
-
bud stage and just before anthesis
-
brenda
-
bud stage and just before anthesis
-
brenda
-
-
brenda
-
-
-
brenda
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
brenda
-
-
brenda
-
-
-
brenda
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
brenda
-
-
-
brenda
-
brenda
-
blue
brenda
-
-
-
brenda
-
-
-
brenda
additional information
-
tissue expression analysis
brenda
additional information
-
tissue expression analysis
-
brenda
additional information
tissue expression analysis
brenda
additional information
-
tissue expression analysis
-
brenda
additional information
-
tissue expression analysis
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
metabolism
overexpression of the acyltransferase gene Vv3AT increases the proportion of acylated anthocyanins in Malbec berries under high temperature
additional information
flavonoid accumulation in the blue- and white-flowered gentian cultivars, overview
additional information
-
flavonoid accumulation in the blue- and white-flowered gentian cultivars, overview
-
additional information
-
flavonoid accumulation in the blue- and white-flowered gentian cultivars, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ANTA_GENTR
469
0
52740
Swiss-Prot
other Location (Reliability: 2 )
A0A2P6S7K6_ROSCH
489
0
54168
TrEMBL
Secretory Pathway (Reliability: 1 )
F4ZG54_MEDTR
468
0
53451
TrEMBL
other Location (Reliability: 3 )
B9RHE8_RICCO
464
0
51884
TrEMBL
other Location (Reliability: 5 )
B9RW82_RICCO
459
0
51645
TrEMBL
other Location (Reliability: 5 )
B9SNQ7_RICCO
458
0
50447
TrEMBL
other Location (Reliability: 2 )
B9SNR0_RICCO
475
0
52850
TrEMBL
other Location (Reliability: 3 )
B9SNQ1_RICCO
351
0
39081
TrEMBL
Mitochondrion (Reliability: 3 )
B9SNQ9_RICCO
477
0
52949
TrEMBL
other Location (Reliability: 2 )
B9SNQ3_RICCO
467
0
51663
TrEMBL
other Location (Reliability: 4 )
B9SNQ4_RICCO
474
0
53054
TrEMBL
other Location (Reliability: 2 )
A0A438GF80_VITVI
261
0
29177
TrEMBL
-
Q3V5Z1_GENTR
365
0
41194
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
52000
-
1 * 52000, SDS-PAGE
57000
-
His-tagged fusion protein, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
monomer
-
1 * 52000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-20°C, 20 mM Tris/HCl, pH 7.0, 0.01 mM (p-aminidinophenyl)methanesulfonyl fluoride, 1 mM DTT, stable for at least 2 years
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
of the recombinant fusion protein using His-tag affinity chromatography
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expression in Escherichia coli as His-tagged fusion protein
-
expression analysis
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Fujiwara, H.; Tanaka, Y.; Fukui, Y.; Nakao, M.; Ashikari, T.; Kusumi, T.
Anthocyanin 5-aromatic acyltransferase from Gentiana triflora
Eur. J. Biochem.
249
45-51
1997
Gentiana triflora
brenda
DAuria, J.C.; Reichelt, M.; Luck, K.; Svatos, A.; Gershenzon, J.
Identification and characterization of the BAHD acyltransferase malonyl CoA: anthocyanidin 5-O-glucoside-6-O-malonyltransferase (At5MAT) in Arabidopsis thaliana
FEBS Lett.
581
872-878
2007
Arabidopsis thaliana
brenda
Griesser, M.; Hoffmann, T.; Bellido, M.L.; Rosati, C.; Fink, B.; Kurtzer, R.; Aharoni, A.; Munoz-Blanco, J.; Schwab, W.
Redirection of flavonoid biosynthesis through the down-regulation of an anthocyanidin glucosyltransferase in ripening strawberry fruit
Plant Physiol.
146
1528-1539
2008
Gentiana scabra x Gentiana triflora, Gentiana triflora (Q3V5Z1), Gentiana triflora Homoi (Q3V5Z1), Gentiana scabra x Gentiana triflora Polano White, Gentiana triflora Maciry (Q3V5Z1)
brenda
de Rosas, I.; Ponce, M.T.; Malovini, E.; Deis, L.; Cavagnaro, B.; Cavagnaro, P.
Loss of anthocyanins and modification of the anthocyanin profiles in grape berries of Malbec and Bonarda grown under high temperature conditions
Plant Sci.
258
137-145
2017
Vitis vinifera (A0A438GF80), Vitis vinifera
brenda
Select items on the left to see more content.
html completed