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Literature summary for 2.3.1.123 extracted from
- Esterification of dolichol in rat liver (1988), J. Biol. Chem., 263, 1347-1352.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| albumin | activates | Rattus norvegicus | |
| Triton X-100 | activates at low concentrations by 50% | Rattus norvegicus |  |
General Stability
| General Stability | Organism |
|---|---|
| Triton X-100 stabilizes | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-octylglycopyranoside | strong, almost complete inactivation at 0.04% | Rattus norvegicus | |
| Ca2+ | 20 mM, 50-60% inhibition | Rattus norvegicus | |
| deoxycholate | strong, almost complete inactivation at 0.04% | Rattus norvegicus | |
| Mn2+ | 20 mM, 50-60% inhibition | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | liver, esterification takes place on cytoplasmic side of membranes of the endoplasmic reticulum and is subsequently distributed to different organelles | Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | KCl and MgCl2 have no influence | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + dolichol | Rattus norvegicus | - |
CoA + dolichyl acetate | - |
? | |
| arachidonoyl-CoA + dolichol | Rattus norvegicus | - |
CoA + dolichyl arachidonate | - |
? | |
| additional information | Rattus norvegicus | esterfication may play a role in targeting the lipid from the endoplasmic reticulum to lysosomes | ? | - |
? | |
| myristoyl-CoA + dolichol | Rattus norvegicus | - |
CoA + dolichyl myristate | - |
? | |
| oleoyl-CoA + dolichol | Rattus norvegicus | - |
CoA + dolichyl oleate | - |
? | |
| palmitoyl-CoA + dolichol | Rattus norvegicus | - |
CoA + dolichyl palmitate | - |
? | |
| stearoyl-CoA + dolichol | Rattus norvegicus | - |
CoA + dolichyl stearate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | slight activity | Rattus norvegicus | - |
| heart | slight activity | Rattus norvegicus | - |
| kidney | slight activity | Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| lung | slight activity | Rattus norvegicus | - |
| muscle | slight activity | Rattus norvegicus | - |
| small intestine | slight activity | Rattus norvegicus | - |
| spleen | slight activity | Rattus norvegicus | - |
| thymus | slight activity | Rattus norvegicus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, microsomes are stable for several months | Rattus norvegicus |
| 4°C, microsomes in 0.25 M sucrose, after 5 days 50% remaining activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + dolichol | - |
Rattus norvegicus | CoA + dolichyl acetate | - |
? | |
| acetyl-CoA + dolichol | 30% of activity compared to palmitoyl-CoA | Rattus norvegicus | CoA + dolichyl acetate | - |
? | |
| arachidonoyl-CoA + dolichol | - |
Rattus norvegicus | CoA + dolichyl arachidonate | - |
? | |
| arachidonoyl-CoA + dolichol | 30% of activity compared to palmitoyl-CoA | Rattus norvegicus | CoA + dolichyl arachidonate | - |
? | |
| additional information | alpha-saturated dolichols are acylated more rapidly than alpha-unsaturated analogues | Rattus norvegicus | ? | - |
? | |
| additional information | esterfication may play a role in targeting the lipid from the endoplasmic reticulum to lysosomes | Rattus norvegicus | ? | - |
? | |
| myristoyl-CoA + dolichol | - |
Rattus norvegicus | CoA + dolichyl myristate | - |
? | |
| myristoyl-CoA + dolichol | 40% of activity compared to palmitoyl-CoA | Rattus norvegicus | CoA + dolichyl myristate | - |
? | |
| oleoyl-CoA + dolichol | - |
Rattus norvegicus | CoA + dolichyl oleate | - |
? | |
| oleoyl-CoA + dolichol | 40% of activity compared to palmitoyl-CoA | Rattus norvegicus | CoA + dolichyl oleate | - |
? | |
| palmitoyl-CoA + dolichol | - |
Rattus norvegicus | CoA + dolichyl palmitate | - |
? | |
| stearoyl-CoA + dolichol | - |
Rattus norvegicus | CoA + dolichyl stearate | - |
? | |
| stearoyl-CoA + dolichol | 40% of activity compared to palmitoyl-CoA | Rattus norvegicus | CoA + dolichyl stearate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
- |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | 20% of maximal activity at pH 5.0, 40% of maximal activity at pH 7.0 | Rattus norvegicus |
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