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Literature summary for 2.3.1.122 extracted from
- The mycolyltransferase 85A, a putative drug target of Mycobacterium tuberculosis: Development of a novel assay and quantification of glycolipid-status of the mycobacterial cell wall (2009), J. Microbiol. Methods, 79, 358-363.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | an enzyme assay using the natural substrate trehalose dimycolate is developed. The colorimetric assay is based on the quantification of glucose from the degradation of trehalose, which is the product from catalytic activity of antigen 85A. This assay is suitable for robust high-throughput screening (HTS) for compound library screening against mycolyltransferase. The assay has a very low coefficient of variance (0.04) in 96-well plates and shows a Z' factor of 0.67-0.73, indicating the robustness of the assay | Mycolicibacterium smegmatis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Mycolicibacterium smegmatis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1296 | - |
alpha,alpha-trehalose 6-monomycolate | pH 5.7, 37°C | Mycolicibacterium smegmatis |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
SDS-PAGE | Mycolicibacterium smegmatis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium smegmatis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography and by applying a linear imidazole gradient from 10 to 500 mM. The final purification step contains gel filtration on a Superdex 200 column | Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha,alpha'-trehalose 6-monomycolate + alpha,alpha'-trehalose 6-monomycolate | - |
Mycolicibacterium smegmatis | alpha,alpha'-trehalose 6,6'-dimycolate + alpha,alpha'-trehalose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| antigen 85A | - |
Mycolicibacterium smegmatis |
| mycolyltransferase | - |
Mycolicibacterium smegmatis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mycolicibacterium smegmatis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.09 | - |
alpha,alpha-trehalose 6-monomycolate | pH 5.7, 37°C | Mycolicibacterium smegmatis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | - |
assay at | Mycolicibacterium smegmatis |
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Release 2023.1 (January 2023)
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