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Literature summary for 2.1.3.10 extracted from

  • Hilbi, H.; Dimroth, P.
    Purification and characterization of a cytoplasmic enzyme component of the Na+-activated malonate decarboxylase system of Malonomonas rubra: acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase (1994), Arch. Microbiol., 162, 48-56.
    View publication on PubMed
Search for more literature for 2.1.3.10

Inhibitors

Inhibitors Comment Organism Structure
citrate
-
 Malonomonas rubra
malonate
-
 Malonomonas rubra

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 malonate pH 6.0 Malonomonas rubra
1.9
-
 malonyl-CoA pH 6.0 Malonomonas rubra
6.9
-
 acetyl-CoA pH 6.0 Malonomonas rubra
54
-
 acetate pH 6.0 Malonomonas rubra

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 gel filtration Malonomonas rubra
67000
-
 1 * 67000, SDS-PAGE Malonomonas rubra

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + dephospho-CoA Malonomonas rubra
-
 CoA + acetyl-dephospho-CoA
-
 r
malonyl-CoA + dephospho-CoA Malonomonas rubra
-
 CoA + malonyl-dephospho-CoA
-
 r
additional information Malonomonas rubra enzyme catalyzes the transfer of acyl carrier protein from acetyl acyl carrier protein and malonate to yield malonyl acyl carrier protein and acetate. Malonate is thus activated on the enzyme by exchange for the catalytically important enzyme-bound acetyl thioester residues ?
-
 ?

Organism

Organism UniProt Comment Textmining
Malonomonas rubra
-
 enzyme is part of the Na+-activied malonate decarboxylase system
-

Purification (Commentary)

Purification (Comment) Organism
purification of subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase Malonomonas rubra

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
367
-
-
 Malonomonas rubra

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + dephospho-CoA
-
 Malonomonas rubra CoA + acetyl-dephospho-CoA
-
 r
acetyl-CoA + malonate
-
 Malonomonas rubra malonyl-CoA + acetate
-
 r
malonyl-CoA + acetate
-
 Malonomonas rubra acetyl-CoA + malonate
-
 r
malonyl-CoA + dephospho-CoA
-
 Malonomonas rubra CoA + malonyl-dephospho-CoA
-
 r
additional information enzyme catalyzes the transfer of acyl carrier protein from acetyl acyl carrier protein and malonate to yield malonyl acyl carrier protein and acetate. Malonate is thus activated on the enzyme by exchange for the catalytically important enzyme-bound acetyl thioester residues Malonomonas rubra ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 67000, SDS-PAGE Malonomonas rubra

Synonyms

Synonyms Comment Organism
acetyl-S-acyl carrier protein:malonate acyl carrier protein-SH transferase
-
 Malonomonas rubra

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Malonomonas rubra

pH Range

pH Minimum pH Maximum Comment Organism
4.5
-
 sharp decrease in activity from pH 5.5 to pH 4.5 Malonomonas rubra

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.4
-
 malonate pH 6.0 Malonomonas rubra
3
-
 citrate pH 6.0 Malonomonas rubra