Literature summary for 2.1.2.9 extracted from

  • Takeuchi, N.; Vial, L.; Panvert, M.; Schmitt, E.; Watanabe, K.; Mechulam, Y.; Blanquet, S.
    Recognition of tRNAs by methionyl-tRNA transformylase from mammalian mitochondria (2001), J. Biol. Chem., 276, 20064-20068.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene encoding mitochondrial enzyme is cloned and expressed in Escherichia coli BL21(DE3) as His-tagged enzyme Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic parameters of the formylation of various mutant methionyl-tRNAs Bos taurus
0.0005
-
E. coli L-methionyl-tRNAfMet
-
Bos taurus
0.0014
-
E. coli L-valyl-tRNAfMet
-
Bos taurus
0.0003
-
E. coli L-isoleucyl-tRNAfMet
-
Bos taurus
0.01
-
E. coli L-methionyl-tRNAmMet above Bos taurus
0.01
-
E. coli L-methionyl-tRNA1Val(CAU) above Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Bos taurus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bos taurus translational initiation in mitochondria ?
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of His-tagged recombinant enzyme, expressed in Escherichia coli BL21(DE3) Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no formylation of Val-tRNA1Met Bos taurus ?
-
?
additional information translational initiation in mitochondria Bos taurus ?
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates bovine mitochondrial and E. coli initiator Met-tRNA with equal efficiency, formylates E. coli elongator Met-tRNA at a lower rate Bos taurus tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O enzyme preferentially recognizes the methionyl moiety of its tRNA substrate, small importance of the tRNA acceptor stem for recognition, E. coli tRNA mutants as substrates Bos taurus tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O broader substrate specificity than that of E. coli enzyme Bos taurus tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O recognition mechanism of methionyl-tRNAfMet Bos taurus tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O mischarged E. coli tRNAfMet Bos taurus tetrahydrofolate + N-formylvalyl-tRNAfMet
-
?
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O less efficiency than with methionyl-tRNAfMet Bos taurus tetrahydrofolate + N-formylvalyl-tRNAfMet
-
?
10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O mischarged E. coli tRNAfMet Bos taurus tetrahydrofolate + N-formylisoleucyl-tRNAfMet
-
?
10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O less efficient than with methionyl-tRNAfMet Bos taurus tetrahydrofolate + N-formylisoleucyl-tRNAfMet
-
?
10-formyltetrahydrofolate + methionyl-tRNAmMet + H2O E. coli elongator Met-tRNAmMet, less efficient than with methionyl-tRNAfMet Bos taurus tetrahydrofolate + N-formylmethionyl-tRNAmMet
-
?
10-formyltetrahydrofolate + methionyl-tRNA1Val(CAU) + H2O mischarged E. coli tRNA1Val(CAU), less efficient than with methionyl-tRNAfMet Bos taurus tetrahydrofolate + N-formylmethionyl-tRNA1Val(CAU)
-
?
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet
-
Bos taurus tetrahydrofolate + E. coli N-formylmethionyl-tRNAfMet
-
r
10-formyltetrahydrofolate + E. coli L-isoleucyl-tRNAfMet
-
Bos taurus tetrahydrofolate + E. coli N-formylisoleucyl-tRNAfMet
-
r
10-formyltetrahydrofolate + E. coli L-valyl-tRNAfMet
-
Bos taurus tetrahydrofolate + E. coli N-formylvalyl-tRNAfMet
-
r
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAmMet
-
Bos taurus tetrahydrofolate + E. coli N-formylmethionyl-tRNAmMet
-
r
10-formyltetrahydrofolate + E. coli L-methionyl-tRNA1Val(CAU)
-
Bos taurus tetrahydrofolate + E. coli N-formylisoleucyl-tRNA1Val(CAU)
-
r
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet
-
Bos taurus tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
r
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAmMet
-
Bos taurus tetrahydrofolate + N-formylmethionyl-tRNAmMet
-
r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information kinetic parameters of the formylation of various mutant methionyl-tRNAs Bos taurus
0.04
-
E. coli L-methionyl-tRNAmMet above Bos taurus
0.03
-
E. coli L-methionyl-tRNA1Val(CAU) above Bos taurus
0.93
-
E. coli L-methionyl-tRNAfMet
-
Bos taurus