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Information on EC 2.1.2.9 - methionyl-tRNA formyltransferase
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EC Tree
2.1.2.9 methionyl-tRNA formyltransferaseSpecify your search results
Word Map
- 2.1.2.9
-
formylation
- leigh
-
methionylated
- eubacteria
-
met-trnafmet
-
anticodon
- met-trnas
-
deformylase
- glycinamide
- n10-formyltetrahydrofolate
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
mtfmt, methionyl-trna formyltransferase, methionyl-trna transformylase, mitochondrial methionyl-trna formyltransferase, methionyl-trna-formyltransferase, fmt1p, formyl-methionyl transferase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
FMT
formyl-methionyl transferase
formylmethionyl-transfer ribonucleic synthetase
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methionyl ribonucleic formyltransferase
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-
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methionyl-transfer ribonucleic transformylase
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-
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methionyl-transfer RNA transformylase
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-
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methionyl-tRNA formyltransferase
methionyl-tRNA Met formyltransferase
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-
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methionyl-tRNA transformylase
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methyltransferase, methionyl-transfer ribonucleate
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mitochondrial methionyl-tRNA formyltransferase
MTF
N10-formyltetrahydrofolic-methionyl-transfer ribonucleic transformylase
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FMT
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MTF
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-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
10-formyltetrahydrofolate + L-methionyl-tRNAfMet = tetrahydrofolate + N-formylmethionyl-tRNAfMet
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
formyl group transfer
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-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
10-formyltetrahydrofolate:L-methionyl-tRNA N-formyltransferase
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CAS REGISTRY NUMBER
COMMENTARY
9015-76-3
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
10-formyltetrahydrofolate + bovine mitochondrial L-methionyl-tRNA
tetrahydrofolate + bovine mitochondrial N-formylmethionyl-tRNA
-
-
-
-
r
10-formyltetrahydrofolate + E. coli L-isoleucyl-tRNAfMet
tetrahydrofolate + E. coli N-formylisoleucyl-tRNAfMet
10-formyltetrahydrofolate + E. coli L-methionyl-tRNA1Val(CAU)
tetrahydrofolate + E. coli N-formylisoleucyl-tRNA1Val(CAU)
-
-
-
-
r
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet
tetrahydrofolate + E. coli N-formylmethionyl-tRNAfMet
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAmMet
tetrahydrofolate + E. coli N-formylmethionyl-tRNAmMet
-
-
-
-
r
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAmMet
tetrahydrofolate + N-formylmethionyl-tRNAmMet
-
-
-
-
r
10-formyltetrahydrofolate + E. coli L-valyl-tRNAfMet
tetrahydrofolate + E. coli N-formylvalyl-tRNAfMet
10-formyltetrahydrofolate + E. coli L-valyl-tRNAfMet
tetrahydrofolate + N-formylvalyl-tRNAfMet
-
-
-
-
r
10-formyltetrahydrofolate + E.coli L-methionyl-tRNAfMet
tetrahydrofolate + E.coli N-formylmethionyl-tRNAfMet
-
-
-
-
r
10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O
tetrahydrofolate + N-formylisoleucyl-tRNAfMet
10-formyltetrahydrofolate + L-isoleucyl-tRNAfMet
tetrahydrofolate + N-formylisoleucyl-tRNAfMet
-
-
-
-
r
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
10-formyltetrahydrofolate + L-methionyl-tRNAMet
tetrahydrofolate + N-formylmethionyl-tRNAMet
10-formyltetrahydrofolate + L-phenylalanyl-tRNAfMet + H2O
tetrahydrofolate + N-formylphenylalanyl-tRNAfMet
-
mutant tRNA G34A36 is a good substrate
-
-
?
10-formyltetrahydrofolate + methionyl-tRNA1Val(CAU) + H2O
tetrahydrofolate + N-formylmethionyl-tRNA1Val(CAU)
-
mischarged E. coli tRNA1Val(CAU), less efficient than with methionyl-tRNAfMet
-
-
?
10-formyltetrahydrofolate + methionyl-tRNAmMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAmMet
-
E. coli elongator Met-tRNAmMet, less efficient than with methionyl-tRNAfMet
-
-
?
10-formyltetrahydrofolate + phenylalanyl-tRNAfMet + H2O
tetrahydrofolate + N-formylphenylalanyl-tRNAfMet
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O
tetrahydrofolate + N-formylvalyl-tRNAfMet
10-formyltetrahydropteroyltriglutamate + L-methionyl-tRNAfMet + H2O
tetrahydropteroyltriglutamate + N-formylmethionyl-tRNAfMet
-
10-formyltetrahydropteroyltriglutamate or 10-formyltetrahydrofolate can act as donor
-
?
10-formyltetrahydrofolate + E. coli L-isoleucyl-tRNAfMet
tetrahydrofolate + E. coli N-formylisoleucyl-tRNAfMet
-
-
-
-
r
10-formyltetrahydrofolate + E. coli L-isoleucyl-tRNAfMet
tetrahydrofolate + E. coli N-formylisoleucyl-tRNAfMet
-
-
-
-
r
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet
tetrahydrofolate + E. coli N-formylmethionyl-tRNAfMet
-
-
-
-
r
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet
tetrahydrofolate + E. coli N-formylmethionyl-tRNAfMet
-
-
-
r
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet
tetrahydrofolate + E. coli N-formylmethionyl-tRNAfMet
-
-
-
-
r
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
-
-
-
r
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
-
-
-
r
10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
-
-
-
r
10-formyltetrahydrofolate + E. coli L-valyl-tRNAfMet
tetrahydrofolate + E. coli N-formylvalyl-tRNAfMet
-
-
-
-
r
10-formyltetrahydrofolate + E. coli L-valyl-tRNAfMet
tetrahydrofolate + E. coli N-formylvalyl-tRNAfMet
-
-
-
-
r
10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O
tetrahydrofolate + N-formylisoleucyl-tRNAfMet
-
mischarged E. coli tRNAfMet
-
-
?
10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O
tetrahydrofolate + N-formylisoleucyl-tRNAfMet
-
less efficient than with methionyl-tRNAfMet
-
-
?
10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O
tetrahydrofolate + N-formylisoleucyl-tRNAfMet
-
mischarged E. coli tRNAfMet
-
-
?
10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O
tetrahydrofolate + N-formylisoleucyl-tRNAfMet
-
250fold reduced catalytic efficiency compared with methionyl-tRNAfMet
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
-
-
-
r
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
-
-
-
r
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
-
-
r
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
Escherichia coli tRNA2fMet
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
-
-
-
r
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
-
-
-
r
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
-
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
human mt-tRNAMet
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
-
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
-
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
-
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
-
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
-
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
formylates bovine mitochondrial and E. coli initiator Met-tRNA with equal efficiency, formylates E. coli elongator Met-tRNA at a lower rate
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
formylates bovine mitochondrial and E. coli initiator Met-tRNA with equal efficiency, formylates E. coli elongator Met-tRNA at a lower rate
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
formylates methionyl-tRNAfMet from E. coli
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
enzyme preferentially recognizes the methionyl moiety of its tRNA substrate, small importance of the tRNA acceptor stem for recognition, E. coli tRNA mutants as substrates
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
broader substrate specificity than that of E. coli enzyme
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
broader substrate specificity than that of E. coli enzyme
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
recognition mechanism of methionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
10-formyltetrahydropteroyltriglutamate or 10-formyltetrahydrofolate can also act as donor
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
specificity is restricted to methionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
involved in initiation of protein biosynthesis
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
Enterococcus faecalis R / ATCC 8043
-
10-formyltetrahydropteroyltriglutamate or 10-formyltetrahydrofolate can also act as donor
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
Enterococcus faecalis R / ATCC 8043
-
specificity is restricted to methionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
Enterococcus faecalis R / ATCC 8043
-
involved in initiation of protein biosynthesis
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
-
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
determinants for formylation are located in the acceptor stem and D-stem of the initiator tRNA, enzyme binds to the tRNA substrate in an induced fit mechanism, tRNA also undergoes induced structural changes during its binding to enzyme
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
role of the linker region between N- and C-terminal domain
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
specificity determinants at the end of the acceptor stem of tRNAfMet
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
specificity determinants at the end of the acceptor stem of tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
specificity determinants at the end of the acceptor stem of tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
affinity of tRNAfMet for enzyme is improved by aminoacylation
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
affinity of tRNAfMet for enzyme is improved by aminoacylation
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
C-terminal region is very important for enzyme activity: basic amino acids contribute mostly toward the nonspecific binding and orientation of the tRNA 3'-end toward the catalytic site
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
C-terminal region is very important for enzyme activity: basic amino acids contribute mostly toward the nonspecific binding and orientation of the tRNA 3'-end toward the catalytic site
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
1.1 mol 10-L-formyltetrahydrofolate is bound per mol monomeric enzyme
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
1.1 mol 10-L-formyltetrahydrofolate is bound per mol monomeric enzyme
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
formylates methionyl-tRNAfMet from E. coli
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
formylates methionyl-tRNAfMet from E. coli
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
formylates methionyl-tRNAfMet from E. coli
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
formylates methionyl-tRNAfMet from E. coli
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
formylates methionyl-tRNAfMet from E. coli
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
invariant Arg-42 within the 16-amino acid insertion loop, residues 34-49, plays a very important role both in steps of substrate binding and in catalysis, Arg-42 makes functional contacts with the determinants in the acceptor stem of the initiator tRNA
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
specificity is restricted to methionyl-tRNAfMet
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
specificity is restricted to methionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
specificity is restricted to methionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
specificity is restricted to methionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
specificity is restricted to methionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
specificity is restricted to methionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
specific for methionyl-tRNAfMet, specificity depends exclusively on the nature of tRNA moiety of aminoacylated tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
nucleotide-mutants of tRNAfMet as substrates
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
tRNA substrate binding
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
tRNA substrate binding
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
recognition mechanism of methionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
recognition mechanism of methionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
recognition mechanism of methionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
important role of a 16-amino acid insertion loop in enzyme for tRNA recognition
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
important role of a 16-amino acid insertion loop in enzyme for tRNA recognition
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
important role of a 16-amino acid insertion loop in enzyme for tRNA recognition
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
important role of a 16-amino acid insertion loop in enzyme for tRNA recognition
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
keeps in store initiator tRNAfMet for initiation of protein synthesis
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
involved in initiation of protein biosynthesis
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
involved in initiation of protein biosynthesis
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
involved in initiation of protein biosynthesis
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
involved in initiation of protein biosynthesis
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
involved in initiation of protein biosynthesis
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
involved in initiation of protein biosynthesis
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
involved in initiation of protein biosynthesis
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
involved in initiation of protein biosynthesis
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
involved in initiation of protein biosynthesis
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
involved in initiation of protein biosynthesis
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
formyl group provides a positive determinant for IF2, which can select the initiator fMet-tRNA from other tRNAs and a negative determinant, which blocks the binding of EF-Tu to the initiator tRNA
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
enzyme reaction irreversibly commits methionyl-tRNAfMet to initiation of translation in eubacteria
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
key step in the targeting of initiator tRNA towards the translation start machinery in prokaryotes
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
small effect of overproduction of MRF on activity of chloramphenicol acetyltransferase
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
Escherichia coli A19
-
specificity is restricted to methionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
Escherichia coli A19
-
involved in initiation of protein biosynthesis
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
Escherichia coli B / ATCC 11303
-
formylates methionyl-tRNAfMet from E. coli
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
recognition mechanism of methionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
important role of a 16-amino acid insertion loop in enzyme for tRNA recognition
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
involved in initiation of protein biosynthesis
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
specificity is restricted to methionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
involved in initiation of protein biosynthesis
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
recognition mechanism of methionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
important role of a 16-amino acid insertion loop in enzyme for tRNA recognition
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
involved in initiation of protein biosynthesis
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
recognition mechanism of methionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
important role of a 16-amino acid insertion loop in enzyme for tRNA recognition
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
involved in initiation of protein biosynthesis
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
specificity is restricted to methionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
involved in initiation of protein biosynthesis
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
role of the linker region between N- and C-terminal domain
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
specificity determinants at the end of the acceptor stem of tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
C-terminal region is very important for enzyme activity: basic amino acids contribute mostly toward the nonspecific binding and orientation of the tRNA 3'-end toward the catalytic site
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
affinity of tRNAfMet for enzyme is improved by aminoacylation
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
1.1 mol 10-L-formyltetrahydrofolate is bound per mol monomeric enzyme
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
formylates methionyl-tRNAfMet from E. coli
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
affinity of tRNAfMet for enzyme is improved by aminoacylation
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
1.1 mol 10-L-formyltetrahydrofolate is bound per mol monomeric enzyme
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
formylates methionyl-tRNAfMet from E. coli
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
specific for methionyl-tRNAfMet, specificity depends exclusively on the nature of tRNA moiety of aminoacylated tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
involved in initiation of protein biosynthesis
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
role of the linker region between N- and C-terminal domain
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
specificity determinants at the end of the acceptor stem of tRNAfMet
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
C-terminal region is very important for enzyme activity: basic amino acids contribute mostly toward the nonspecific binding and orientation of the tRNA 3'-end toward the catalytic site
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
formylates methionyl-tRNAfMet from Euglena gracilis or yeast
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
formylates methionyl-tRNAfMet from E. coli
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
high specificity for initiator tRNAMet, strong positive cooperativity for Met-tRNAfMet and N10-formyltetrahydrofolate
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
involved in initiation of protein synthesis in chloroplasts, other mechanism than in cytoplasm of eukaryotes
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
formylates methionyl-tRNAfMet from Euglena gracilis or yeast
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
formylates methionyl-tRNAfMet from E. coli
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
high specificity for initiator tRNAMet, strong positive cooperativity for Met-tRNAfMet and N10-formyltetrahydrofolate
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
involved in initiation of protein synthesis in chloroplasts, other mechanism than in cytoplasm of eukaryotes
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
formylates methionyl-tRNAfMet from E. coli
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
Asn-107, His-109 and Asp-145 are catalytic residues, Arg-41 is involved in tRNA recognition
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
E. coli tRNAFMet
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
formylates yeast mitochondrial methionyl-tRNAfMet, specific for initiator tRNAs
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
formylates methionyl-tRNAfMet from E. coli
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
enzyme recognizes nucleotidic features in the acceptor stem of mitochondrial initiator tRNA, recognition mechanism
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
initiation of protein synthesis in mitochondria
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
the dispensable mitochondrial methionyl-tRNAformyltransferase, Fmt1p, is essential for respiratory growth in the absence of Rsm28p
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
formylates methionyl-tRNAfMet from wheat germ and leaf
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
formylates methionyl-tRNAfMet from E. coli
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
component of a protein chain initiating system in chloroplasts
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O
tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
formylation of the methionyl-tRNA-fMet is essential for in vitro binding of imported formylated elongator-type tRNA(Met)
-
-
?
10-formyltetrahydrofolate + L-methionyl-tRNAMet
tetrahydrofolate + N-formylmethionyl-tRNAMet
-
-
-
-
r
10-formyltetrahydrofolate + L-methionyl-tRNAMet
tetrahydrofolate + N-formylmethionyl-tRNAMet
Escherichia coli B / ATCC 11303
-
-
-
-
r
10-formyltetrahydrofolate + phenylalanyl-tRNAfMet + H2O
tetrahydrofolate + N-formylphenylalanyl-tRNAfMet
-
mischarged E. coli tRNAfMet, reduced velocity compared to the formylation of methionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + phenylalanyl-tRNAfMet + H2O
tetrahydrofolate + N-formylphenylalanyl-tRNAfMet
-
7fold less efficient formylation than of methionyl-tRNAfMet
-
-
?
10-formyltetrahydrofolate + phenylalanyl-tRNAfMet + H2O
tetrahydrofolate + N-formylphenylalanyl-tRNAfMet
-
mischarged E. coli tRNAfMet, reduced velocity compared to the formylation of methionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O
tetrahydrofolate + N-formylvalyl-tRNAfMet
-
mischarged E. coli tRNAfMet
-
-
?
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O
tetrahydrofolate + N-formylvalyl-tRNAfMet
-
less efficiency than with methionyl-tRNAfMet
-
-
?
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O
tetrahydrofolate + N-formylvalyl-tRNAfMet
-
mischarged E. coli tRNAfMet
-
?
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O
tetrahydrofolate + N-formylvalyl-tRNAfMet
-
mischarged E. coli tRNAfMet
-
-
?
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O
tetrahydrofolate + N-formylvalyl-tRNAfMet
-
130fold less efficient formylation than of methionyl-tRNAfMet
-
-
?
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O
tetrahydrofolate + N-formylvalyl-tRNAfMet
-
reduced velocity compared to the formylation of methionyl-tRNAfMet
-
?
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O
tetrahydrofolate + N-formylvalyl-tRNAfMet
-
mischarged E. coli tRNAfMet
-
?
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O
tetrahydrofolate + N-formylvalyl-tRNAfMet
-
mischarged E. coli tRNAfMet
-
-
?
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O
tetrahydrofolate + N-formylvalyl-tRNAfMet
-
25fold reduced catalytic efficiency compared with methionyl-tRNAfMet
-
-
?
additional information
?
-
-
no formylation of methionyl-tRNAmMet
-
-
?
additional information
?
-
-
ATP plus L-methionine and either tRNAmMet or tRNAfMet cannot replace methionyl-tRNAfMet
-
-
?
additional information
?
-
Enterococcus faecalis R / ATCC 8043
-
no formylation of methionyl-tRNAmMet
-
-
?
additional information
?
-
Enterococcus faecalis R / ATCC 8043
-
ATP plus L-methionine and either tRNAmMet or tRNAfMet cannot replace methionyl-tRNAfMet
-
-
?
additional information
?
-
-
no formyl group donor: 5-formyltetrahydrofolate
-
-
?