Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Idiomarina loihiensis |
Protein Variants | Comment | Organism |
---|---|---|
G132P | the mutant shows 301% activity compared to the wild type enzyme | Idiomarina loihiensis |
H61G | the mutant shows 283% activity compared to the wild type enzyme | Idiomarina loihiensis |
H61G/G132P | the mutant shows 356% activity compared to the wild type enzyme | Idiomarina loihiensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
DL-phenylserine | wild type enzyme, at pH 7.0 and 37°C | Idiomarina loihiensis | |
81.9 | - |
DL-phenylserine | mutant enzyme G132P, at pH 7.0 and 37°C | Idiomarina loihiensis | |
93.2 | - |
DL-phenylserine | mutant enzyme H61G/G132P, at pH 7.0 and 37°C | Idiomarina loihiensis | |
131.6 | - |
DL-phenylserine | mutant enzyme H61G, at pH 7.0 and 37°C | Idiomarina loihiensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | Idiomarina loihiensis | - |
tetrahydrofolate + L-serine | - |
r | |
tetrahydrofolate + L-serine | Idiomarina loihiensis | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Idiomarina loihiensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose resin column chromatography and Superdex S200 gel filtration | Idiomarina loihiensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Idiomarina loihiensis | tetrahydrofolate + L-serine | - |
r | |
DL-phenylserine + ? | - |
Idiomarina loihiensis | benzaldehyde + ? | - |
? | |
tetrahydrofolate + L-serine | - |
Idiomarina loihiensis | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 45400, SDS-PAGE and calculated from amino acid sequence | Idiomarina loihiensis |
Synonyms | Comment | Organism |
---|---|---|
GlyA | - |
Idiomarina loihiensis |
serine hydroxymethyl transferase | - |
Idiomarina loihiensis |
SHMT | - |
Idiomarina loihiensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Idiomarina loihiensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 60 | more than 45% activity between 35 and 60°C | Idiomarina loihiensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 65 | the wild type enzyme shows a rapid decrease (to less than 20%) in activity after 30 min at 55-65°C, and shows about 35% activity after 30 min at 45°C | Idiomarina loihiensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.51 | - |
DL-phenylserine | wild type enzyme, at pH 7.0 and 37°C | Idiomarina loihiensis | |
2.34 | - |
DL-phenylserine | mutant enzyme H61G, at pH 7.0 and 37°C | Idiomarina loihiensis | |
3.67 | - |
DL-phenylserine | mutant enzyme G132P, at pH 7.0 and 37°C | Idiomarina loihiensis | |
6.89 | - |
DL-phenylserine | mutant enzyme H61G/G132P, at pH 7.0 and 37°C | Idiomarina loihiensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Idiomarina loihiensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | about 20% activity at pH 6.5, about 85% activity at pH 7.0, about 95% activity at pH 8.0, about 75% activity at pH 9.0, about 45% activity at pH 10.0 | Idiomarina loihiensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Idiomarina loihiensis |