Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
expressed in Escherichia coli BL21(DE3) cells | Plasmodium vivax |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tetrahydrofolate | strong and pH-dependent substrate inhibition due to the formation of the enzyme-glycine:tetrahydrofolate dead-end complex (at low pH values) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
tetrahydrofolate | at pH 6.6, temperature not specified in the publication | Homo sapiens | |
0.011 | - |
tetrahydrofolate | at pH 7.1, temperature not specified in the publication | Homo sapiens | |
0.018 | - |
tetrahydrofolate | at pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.038 | - |
tetrahydrofolate | at pH 8.3, temperature not specified in the publication | Plasmodium vivax | |
0.042 | - |
tetrahydrofolate | at pH 7.9, temperature not specified in the publication | Plasmodium vivax | |
0.048 | - |
tetrahydrofolate | at pH 7.1, temperature not specified in the publication | Plasmodium vivax | |
0.049 | - |
tetrahydrofolate | at pH 7.9, temperature not specified in the publication | Homo sapiens | |
0.051 | - |
tetrahydrofolate | at pH 6.6, temperature not specified in the publication | Plasmodium vivax | |
0.054 | - |
tetrahydrofolate | at pH 7.5, temperature not specified in the publication | Plasmodium vivax | |
0.094 | - |
tetrahydrofolate | at pH 8.3, temperature not specified in the publication | Homo sapiens | |
0.11 | - |
L-serine | at pH 7.9, temperature not specified in the publication | Plasmodium vivax | |
0.12 | - |
L-serine | at pH 8.3, temperature not specified in the publication | Plasmodium vivax | |
0.16 | - |
L-serine | at pH 8.3, temperature not specified in the publication | Homo sapiens | |
0.18 | - |
L-serine | at pH 7.9, temperature not specified in the publication | Homo sapiens | |
0.2 | - |
L-serine | at pH 7.5, temperature not specified in the publication | Plasmodium vivax | |
0.22 | - |
L-serine | at pH 7.5, temperature not specified in the publication | Homo sapiens | |
0.23 | - |
L-serine | at pH 7.1, temperature not specified in the publication | Plasmodium vivax | |
0.28 | - |
L-serine | at pH 7.1, temperature not specified in the publication | Homo sapiens | |
0.3 | - |
L-serine | at pH 6.6, temperature not specified in the publication | Plasmodium vivax | |
0.42 | - |
L-serine | at pH 6.6, temperature not specified in the publication | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | Homo sapiens | - |
tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | Plasmodium vivax | - |
tetrahydrofolate + L-serine | - |
r | |
tetrahydrofolate + L-serine | Homo sapiens | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
tetrahydrofolate + L-serine | Plasmodium vivax | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Plasmodium vivax | - |
- |
- |
Purification (Comment) | Organism |
---|---|
polyethyleneimine precipitation, Q-Sepharose column chromatography and Sephacyl S-200 gel filtration | Homo sapiens |
polyethyleneimine precipitation, SP-Sepharose column chromatography and DEAE-Sepharose column chromatography | Plasmodium vivax |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Homo sapiens | tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Plasmodium vivax | tetrahydrofolate + L-serine | - |
r | |
tetrahydrofolate + L-serine | - |
Homo sapiens | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
tetrahydrofolate + L-serine | - |
Plasmodium vivax | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Homo sapiens |
serine hydroxymethyltransferase | - |
Plasmodium vivax |
SHMT | - |
Homo sapiens |
SHMT | - |
Plasmodium vivax |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
L-serine | at pH 8.3, temperature not specified in the publication | Plasmodium vivax | |
0.41 | - |
tetrahydrofolate | at pH 8.3, temperature not specified in the publication | Plasmodium vivax | |
0.57 | - |
L-serine | at pH 7.9, temperature not specified in the publication | Plasmodium vivax | |
0.57 | - |
tetrahydrofolate | at pH 7.9, temperature not specified in the publication | Plasmodium vivax | |
0.81 | - |
L-serine | at pH 7.5, temperature not specified in the publication | Plasmodium vivax | |
0.81 | - |
tetrahydrofolate | at pH 7.5, temperature not specified in the publication | Plasmodium vivax | |
1.1 | - |
L-serine | at pH 6.6, temperature not specified in the publication | Plasmodium vivax | |
1.1 | - |
tetrahydrofolate | at pH 6.6, temperature not specified in the publication | Plasmodium vivax | |
1.41 | - |
L-serine | at pH 7.1, temperature not specified in the publication | Plasmodium vivax | |
1.41 | - |
tetrahydrofolate | at pH 7.1, temperature not specified in the publication | Plasmodium vivax | |
19.6 | - |
L-serine | at pH 8.3, temperature not specified in the publication | Homo sapiens | |
19.6 | - |
tetrahydrofolate | at pH 8.3, temperature not specified in the publication | Homo sapiens | |
19.8 | - |
L-serine | at pH 7.5, temperature not specified in the publication | Homo sapiens | |
19.8 | - |
tetrahydrofolate | at pH 7.5, temperature not specified in the publication | Homo sapiens | |
19.8 | - |
L-serine | at pH 7.9, temperature not specified in the publication | Homo sapiens | |
19.8 | - |
tetrahydrofolate | at pH 7.9, temperature not specified in the publication | Homo sapiens | |
21 | - |
L-serine | at pH 7.1, temperature not specified in the publication | Homo sapiens | |
21 | - |
tetrahydrofolate | at pH 7.1, temperature not specified in the publication | Homo sapiens | |
30 | - |
L-serine | at pH 6.6, temperature not specified in the publication | Homo sapiens | |
30 | - |
tetrahydrofolate | at pH 6.6, temperature not specified in the publication | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
- |
Homo sapiens |
7 | - |
- |
Plasmodium vivax |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.6 | 8.4 | the catalytic efficiency of the enzyme for tetrahydrofolate is about 29fold greater at pH 6.6 than at pH 8.3 | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Homo sapiens | |
pyridoxal 5'-phosphate | - |
Plasmodium vivax |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
tetrahydrofolate | at pH 6.6, temperature not specified in the publication | Homo sapiens | |
0.16 | - |
tetrahydrofolate | at pH 7.1, temperature not specified in the publication | Homo sapiens | |
0.27 | - |
tetrahydrofolate | at pH 7.5, temperature not specified in the publication | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
L-serine | at pH 8.3, temperature not specified in the publication | Plasmodium vivax | |
3.7 | - |
L-serine | at pH 6.6, temperature not specified in the publication | Plasmodium vivax | |
4.1 | - |
L-serine | at pH 7.5, temperature not specified in the publication | Plasmodium vivax | |
5.2 | - |
L-serine | at pH 7.9, temperature not specified in the publication | Plasmodium vivax | |
6.1 | - |
L-serine | at pH 7.1, temperature not specified in the publication | Plasmodium vivax | |
11 | - |
tetrahydrofolate | at pH 8.3, temperature not specified in the publication | Plasmodium vivax | |
14 | - |
tetrahydrofolate | at pH 7.9, temperature not specified in the publication | Plasmodium vivax | |
15 | - |
tetrahydrofolate | at pH 7.5, temperature not specified in the publication | Plasmodium vivax | |
22 | - |
tetrahydrofolate | at pH 6.6, temperature not specified in the publication | Plasmodium vivax | |
29 | - |
tetrahydrofolate | at pH 7.1, temperature not specified in the publication | Plasmodium vivax | |
71 | - |
L-serine | at pH 6.6, temperature not specified in the publication | Homo sapiens | |
75 | - |
L-serine | at pH 7.1, temperature not specified in the publication | Homo sapiens | |
90 | - |
L-serine | at pH 7.5, temperature not specified in the publication | Homo sapiens | |
110 | - |
L-serine | at pH 7.9, temperature not specified in the publication | Homo sapiens | |
123 | - |
L-serine | at pH 8.3, temperature not specified in the publication | Homo sapiens | |
209 | - |
tetrahydrofolate | at pH 8.3, temperature not specified in the publication | Homo sapiens | |
404 | - |
tetrahydrofolate | at pH 7.9, temperature not specified in the publication | Homo sapiens | |
1100 | - |
tetrahydrofolate | at pH 7.5, temperature not specified in the publication | Homo sapiens | |
1909 | - |
tetrahydrofolate | at pH 7.1, temperature not specified in the publication | Homo sapiens | |
6000 | - |
tetrahydrofolate | at pH 6.6, temperature not specified in the publication | Homo sapiens |