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show all sequences of 2.1.1.86

Role of the fused corrinoid/methyl transfer protein CmtA during CO-dependent growth of Methanosarcina acetivorans

Vepachedu, V.R.; Ferry, J.G.; J. Bacteriol. 194, 4161-4168 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli Rosetta (DE3) cells
Methanosarcina acetivorans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.135
-
5-Methyl-5,6,7,8-tetrahydromethanopterin
at 37°C in 50 mM MOPS-KOH (pH 7.0)
Methanosarcina acetivorans
0.277
-
coenzyme M
at 37°C in 50 mM MOPS-KOH (pH 7.0)
Methanosarcina acetivorans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
CmtA
Methanosarcina acetivorans
5737
-
membrane
MtrA-H
Methanosarcina acetivorans
16020
-
soluble
CmtA
Methanosarcina acetivorans
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
69000
-
x * 69000, SDS-PAGE
Methanosarcina acetivorans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M
Methanosarcina acetivorans
-
5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M
-
-
?
5-methyl-5,6,7,8-tetrahydrosarcinapterin + coenzyme M
Methanosarcina acetivorans
-
5,6,7,8-tetrahydrosarcinapterin + methyl-coenzyme M
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanosarcina acetivorans
Q8THX4
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5,6,7,8-tetrahydromethanopterin + methylcob(III)alamin
-
734072
Methanosarcina acetivorans
5-methyl-5,6,7,8-tetrahydromethanopterin + cob(III)alamin
-
-
-
?
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M
-
734072
Methanosarcina acetivorans
5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M
-
-
-
?
5-methyl-5,6,7,8-tetrahydrosarcinapterin + coenzyme M
-
734072
Methanosarcina acetivorans
5,6,7,8-tetrahydrosarcinapterin + methyl-coenzyme M
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 69000, SDS-PAGE
Methanosarcina acetivorans
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli Rosetta (DE3) cells
Methanosarcina acetivorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.135
-
5-Methyl-5,6,7,8-tetrahydromethanopterin
at 37°C in 50 mM MOPS-KOH (pH 7.0)
Methanosarcina acetivorans
0.277
-
coenzyme M
at 37°C in 50 mM MOPS-KOH (pH 7.0)
Methanosarcina acetivorans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
CmtA
Methanosarcina acetivorans
5737
-
membrane
MtrA-H
Methanosarcina acetivorans
16020
-
soluble
CmtA
Methanosarcina acetivorans
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
69000
-
x * 69000, SDS-PAGE
Methanosarcina acetivorans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M
Methanosarcina acetivorans
-
5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M
-
-
?
5-methyl-5,6,7,8-tetrahydrosarcinapterin + coenzyme M
Methanosarcina acetivorans
-
5,6,7,8-tetrahydrosarcinapterin + methyl-coenzyme M
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5,6,7,8-tetrahydromethanopterin + methylcob(III)alamin
-
734072
Methanosarcina acetivorans
5-methyl-5,6,7,8-tetrahydromethanopterin + cob(III)alamin
-
-
-
?
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M
-
734072
Methanosarcina acetivorans
5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M
-
-
-
?
5-methyl-5,6,7,8-tetrahydrosarcinapterin + coenzyme M
-
734072
Methanosarcina acetivorans
5,6,7,8-tetrahydrosarcinapterin + methyl-coenzyme M
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 69000, SDS-PAGE
Methanosarcina acetivorans
General Information
General Information
Commentary
Organism
physiological function
CmtA is a soluble 5-methyl-5,6,7,8-tetrahydrosarcinapterin:coenzyme M methyltransferase postulated to supplement the membrane-bound 5-methyl-5,6,7,8-tetrahydromethanopterin:coenzyme M methyltransferase during CO-dependent growth of Methanosarcina acetivorans
Methanosarcina acetivorans
General Information (protein specific)
General Information
Commentary
Organism
physiological function
CmtA is a soluble 5-methyl-5,6,7,8-tetrahydrosarcinapterin:coenzyme M methyltransferase postulated to supplement the membrane-bound 5-methyl-5,6,7,8-tetrahydromethanopterin:coenzyme M methyltransferase during CO-dependent growth of Methanosarcina acetivorans
Methanosarcina acetivorans
Other publictions for EC 2.1.1.86
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733227
Peterson
Towards a computational model ...
Methanosarcina acetivorans
Archaea
2014
898453
2014
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2
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2
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2
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2
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2
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734072
Vepachedu
Role of the fused corrinoid/me ...
Methanosarcina acetivorans
J. Bacteriol.
194
4161-4168
2012
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1
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2
3
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1
2
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3
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3
1
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1
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2
3
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1
2
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3
1
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1
1
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687410
Welander
Mutagenesis of the C1 oxidatio ...
Methanosarcina barkeri
J. Bacteriol.
190
1928-1936
2008
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1
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2
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1
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727499
Lienard
Cloning, sequencing and expres ...
Methanosarcina mazei, Methanosarcina mazei DSM 3647
FEBS Lett.
425
204-208
1998
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1
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1
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6
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2
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1
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2
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727407
Lienard
Sodium ion translocation by N5 ...
Methanosarcina mazei, Methanosarcina mazei DSM 3647
Eur. J. Biochem.
239
857-864
1996
-
-
-
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-
1
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1
1
7
2
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3
-
-
1
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1
1
-
8
1
1
-
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-
1
-
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1
-
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-
1
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-
1
-
-
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1
1
7
2
-
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1
-
1
1
-
8
1
1
-
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-
1
-
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-
-
-
-
-
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-
639489
Sauer
Tetrahydromethanopterin methyl ...
Methanothermobacter thermautotrophicus
Biochem. Biophys. Res. Commun.
136
542-547
1986
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2
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1
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2
1
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2
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1
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1
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1
1
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2
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1
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1
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2
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1
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1
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1
1
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