BRENDA - Enzyme Database
show all sequences of 2.1.1.86

Sodium ion translocation by N5-methyltetrahydromethanopterin: coenzyme M methyltransferase from Methanosarcina mazei Gö1 reconstituted in ether lipid liposomes

Lienard, T.; Becher, B.; Marschall, M.; Bowien, S.; Gottschalk, G.; Eur. J. Biochem. 239, 857-864 (1996)

Data extracted from this reference:

General Stability
General Stability
Organism
during purification, the enzyme is stabilized with 0.01 mM hydroxocobalamin
Methanosarcina mazei
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane associated enzyme
Methanosarcina mazei
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Na+
maximum activity at 1 mM Na+
Methanosarcina mazei
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
9000
-
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE
Methanosarcina mazei
12000
-
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE
Methanosarcina mazei
13000
-
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE
Methanosarcina mazei
20000
-
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE
Methanosarcina mazei
28000
-
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE
Methanosarcina mazei
34000
-
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE
Methanosarcina mazei
380000
-
non-denaturing gradient PAGE
Methanosarcina mazei
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
Methanosarcina mazei
2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
-
?
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
Methanosarcina mazei DSM 3647
2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Methanosarcina mazei
P80655 and P80653 and P80651 and P80654 and P80656 and P80650
P80655: subunit B, P80653: subunit D, P80651: subunit E, P80654: subunit F, P80656: subunit G, P80650: subunit H
-
Methanosarcina mazei DSM 3647
P80655 and P80653 and P80651 and P80654 and P80656 and P80650
P80655: subunit B, P80653: subunit D, P80651: subunit E, P80654: subunit F, P80656: subunit G, P80650: subunit H
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Methanosarcina mazei
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:acetate-grown cell
-
Methanosarcina mazei
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
10.4
-
37°C, pH 6.9
Methanosarcina mazei
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump
727407
Methanosarcina mazei
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
-
-
?
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump. 1.7 mol Na+ per mol of methyl groups transferred are translocated
727407
Methanosarcina mazei
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
-
-
?
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump
727407
Methanosarcina mazei DSM 3647
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
-
-
?
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump. 1.7 mol Na+ per mol of methyl groups transferred are translocated
727407
Methanosarcina mazei DSM 3647
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
-
-
?
methylcobalamin + 2-mercaptoethanesulfonate
at 13.6% of the activity compared to 5-methyl-5,6,7,8-tetrahydromethanopterin
727407
Methanosarcina mazei
?
-
-
-
?
methylcobalamin + 2-mercaptoethanesulfonate
at 13.6% of the activity compared to 5-methyl-5,6,7,8-tetrahydromethanopterin
727407
Methanosarcina mazei DSM 3647
?
-
-
-
?
N5-methyltetrahydrofolate + 2-mercaptoethanesulfonate
at 31.8% of the activity compared to 5-methyl-5,6,7,8-tetrahydromethanopterin
727407
Methanosarcina mazei
?
-
-
-
?
N5-methyltetrahydrofolate + 2-mercaptoethanesulfonate
at 31.8% of the activity compared to 5-methyl-5,6,7,8-tetrahydromethanopterin
727407
Methanosarcina mazei DSM 3647
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE
Methanosarcina mazei
Synonyms
Synonyms
Commentary
Organism
H,MPT:coenzyme M methyltransferase
-
Methanosarcina mazei
N5-methyltetrahydromethanopterin: coenzyme M methyltransferase
-
Methanosarcina mazei
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Methanosarcina mazei
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
-
Methanosarcina mazei
Cofactor
Cofactor
Commentary
Organism
Structure
corrinoid
-
Methanosarcina mazei
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
corrinoid
-
Methanosarcina mazei
General Stability (protein specific)
General Stability
Organism
during purification, the enzyme is stabilized with 0.01 mM hydroxocobalamin
Methanosarcina mazei
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane associated enzyme
Methanosarcina mazei
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Na+
maximum activity at 1 mM Na+
Methanosarcina mazei
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
9000
-
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE
Methanosarcina mazei
12000
-
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE
Methanosarcina mazei
13000
-
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE
Methanosarcina mazei
20000
-
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE
Methanosarcina mazei
28000
-
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE
Methanosarcina mazei
34000
-
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE
Methanosarcina mazei
380000
-
non-denaturing gradient PAGE
Methanosarcina mazei
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
Methanosarcina mazei
2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
-
?
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
Methanosarcina mazei DSM 3647
2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Methanosarcina mazei
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:acetate-grown cell
-
Methanosarcina mazei
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
10.4
-
37°C, pH 6.9
Methanosarcina mazei
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump
727407
Methanosarcina mazei
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
-
-
?
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump. 1.7 mol Na+ per mol of methyl groups transferred are translocated
727407
Methanosarcina mazei
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
-
-
?
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump
727407
Methanosarcina mazei DSM 3647
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
-
-
?
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump. 1.7 mol Na+ per mol of methyl groups transferred are translocated
727407
Methanosarcina mazei DSM 3647
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
-
-
?
methylcobalamin + 2-mercaptoethanesulfonate
at 13.6% of the activity compared to 5-methyl-5,6,7,8-tetrahydromethanopterin
727407
Methanosarcina mazei
?
-
-
-
?
methylcobalamin + 2-mercaptoethanesulfonate
at 13.6% of the activity compared to 5-methyl-5,6,7,8-tetrahydromethanopterin
727407
Methanosarcina mazei DSM 3647
?
-
-
-
?
N5-methyltetrahydrofolate + 2-mercaptoethanesulfonate
at 31.8% of the activity compared to 5-methyl-5,6,7,8-tetrahydromethanopterin
727407
Methanosarcina mazei
?
-
-
-
?
N5-methyltetrahydrofolate + 2-mercaptoethanesulfonate
at 31.8% of the activity compared to 5-methyl-5,6,7,8-tetrahydromethanopterin
727407
Methanosarcina mazei DSM 3647
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE
Methanosarcina mazei
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Methanosarcina mazei
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
-
Methanosarcina mazei
Other publictions for EC 2.1.1.86
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733227
Peterson
Towards a computational model ...
Methanosarcina acetivorans
Archaea
2014
898453
2014
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
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-
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2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734072
Vepachedu
Role of the fused corrinoid/me ...
Methanosarcina acetivorans
J. Bacteriol.
194
4161-4168
2012
-
-
1
-
-
-
-
2
3
-
1
2
-
2
-
-
-
-
-
-
-
-
3
1
9
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
3
-
1
2
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
687410
Welander
Mutagenesis of the C1 oxidatio ...
Methanosarcina barkeri
J. Bacteriol.
190
1928-1936
2008
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-
-
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1
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-
-
-
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-
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1
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-
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2
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1
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-
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-
-
-
-
-
-
-
-
-
727499
Lienard
Cloning, sequencing and expres ...
Methanosarcina mazei, Methanosarcina mazei DSM 3647
FEBS Lett.
425
204-208
1998
-
-
1
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727407
Lienard
Sodium ion translocation by N5 ...
Methanosarcina mazei, Methanosarcina mazei DSM 3647
Eur. J. Biochem.
239
857-864
1996
-
-
-
-
-
1
-
-
1
1
7
2
-
2
-
-
1
-
-
1
1
-
8
1
2
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
1
7
2
-
-
-
1
-
1
1
-
8
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
639489
Sauer
Tetrahydromethanopterin methyl ...
Methanothermobacter thermautotrophicus
Biochem. Biophys. Res. Commun.
136
542-547
1986
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-
-
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-
-
-
2
-
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1
-
2
1
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-
-
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2
-
-
1
-
1
-
1
1
-
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-
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-
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-
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-
-
-
-
-
-
2
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-
1
-
1
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-
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-
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-
2
-
1
-
1
-
1
1
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-
-
-
-
-
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