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Literature summary for 2.1.1.85 extracted from

  • Dai, S.; Horton, J.R.; Woodcock, C.B.; Wilkinson, A.W.; Zhang, X.; Gozani, O.; Cheng, X.
    Structural basis for the target specificity of actin histidine methyltransferase SETD3 (2019), Nat. Commun., 10, 3541 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.85

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6 and 30% (w/v) polyethylene glycol 4000 Homo sapiens

Protein Variants

Protein Variants Comment Organism
N255A the mutant has increased lysine methylation activity and decreased histidine methylation activity compared to the wild type enzyme Homo sapiens
N255V the mutant has increased lysine methylation activity and decreased histidine methylation activity compared to the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 synthetic peptide corresponding to residue 66-80 of actin mutant enzyme N255A, at pH 10.5 and 37°C Homo sapiens
0.0092
-
 synthetic peptide corresponding to residue 66-80 of actin mutant enzyme N255V, at pH 10.5 and 37°C Homo sapiens
0.021
-
 actin L-histidine73 wild type enzyme, at pH 10.5 and 37°C Homo sapiens
0.43
-
 synthetic peptide corresponding to residue 66-80 of actin wild type enzyme, at pH 10.5 and 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q86TU7
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography, HiTrap Q column chromatography, and Superdex S200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme has weak lysine methylation activity on an actin peptide in which the target His73 is substituted by a L-lysine Homo sapiens ?
-
-
S-adenosyl-L-methionine + actin L-histidine73 in a pre-reactive complex the enzyme binds the N3-protonated form of actin His73, and in a post-reactive product complex, the enzyme generates the methylated histidine in an N1-protonated and N3-methylated form Homo sapiens S-adenosyl-L-homocysteine + actin Ntau-methyl-L-histidine73
-
 ?
S-adenosyl-L-methionine + synthetic peptide corresponding to residue 66-80 of actin
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
actin histidine methyltransferase
-
 Homo sapiens
actin histidine-N3 methyltransferase
-
 Homo sapiens
protein (actin) histidine MTase
-
 Homo sapiens
setd3
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00017
-
 synthetic peptide corresponding to residue 66-80 of actin wild type enzyme, at pH 10.5 and 37°C Homo sapiens
0.00036
-
 synthetic peptide corresponding to residue 66-80 of actin mutant enzyme N255V, at pH 10.5 and 37°C Homo sapiens
0.0012
-
 synthetic peptide corresponding to residue 66-80 of actin mutant enzyme N255A, at pH 10.5 and 37°C Homo sapiens
0.006
-
 actin L-histidine73 wild type enzyme, at pH 10.5 and 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00036
-
 synthetic peptide corresponding to residue 66-80 of actin wild type enzyme, at pH 10.5 and 37°C Homo sapiens
0.039
-
 synthetic peptide corresponding to residue 66-80 of actin mutant enzyme N255V, at pH 10.5 and 37°C Homo sapiens
0.203
-
 synthetic peptide corresponding to residue 66-80 of actin mutant enzyme N255A, at pH 10.5 and 37°C Homo sapiens
0.28
-
 actin L-histidine73 wild type enzyme, at pH 10.5 and 37°C Homo sapiens