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Information on EC 2.1.1.85 - protein-histidine N-methyltransferase for references in articles please use BRENDA:EC2.1.1.85
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EC Tree
IUBMB Comments Highly specific for histidine residues, for example, in actin.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
actin-specific histidine methyltransferase, methyltransferase, protein (histidine), protein methylase IV, S-adenosyl methionine:protein-histidine N-methyltransferase,
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actin-specific histidine methyltransferase
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methyltransferase, protein (histidine)
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protein methylase IV
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S-adenosyl methionine:protein-histidine N-methyltransferase
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S-adenosyl-L-methionine + protein L-histidine = S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
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methyl group transfer
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S-adenosyl-L-methionine:protein-L-histidine N-tele-methyltransferase
Highly specific for histidine residues, for example, in actin.
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S-adenosyl-L-methionine + protein L-histidine
S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
S-adenosyl-L-methionine + synthetic peptide corresponding to residue 69-77 of actin
S-adenosyl-L-homocysteine + peptide Ntau-methyl-L-histidine
S-adenosyl-L-methionine + protein L-histidine
S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
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?
S-adenosyl-L-methionine + protein L-histidine
S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
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specifically methylates histidine residues of actin
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S-adenosyl-L-methionine + protein L-histidine
S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
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specifically methylates histidine residues of actin
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S-adenosyl-L-methionine + protein L-histidine
S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
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some activity with gluten
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S-adenosyl-L-methionine + protein L-histidine
S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
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negligible activity with: bovine serum albumin, fibrinogen, casein, histone type IIA, gelatin, human gamma-globulin
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?
S-adenosyl-L-methionine + protein L-histidine
S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
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naturally occuring actins are poor substrates, due to preexistent methylation at His 73
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?
S-adenosyl-L-methionine + protein L-histidine
S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
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chicken beta-actin expressed in E. coli as a fusion protein with 80 amino acids of an influenza protein, NS1
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S-adenosyl-L-methionine + synthetic peptide corresponding to residue 69-77 of actin
S-adenosyl-L-homocysteine + peptide Ntau-methyl-L-histidine
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S-adenosyl-L-methionine + synthetic peptide corresponding to residue 69-77 of actin
S-adenosyl-L-homocysteine + peptide Ntau-methyl-L-histidine
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Tyr-Pro-Ile-Glu-His-Gly-Ile-Ile-Thr
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S-adenosyl-L-methionine + protein L-histidine
S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
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S-adenosyl-L-methionine + synthetic peptide corresponding to residue 69-77 of actin
S-adenosyl-L-homocysteine + peptide Ntau-methyl-L-histidine
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additional information
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no requirement for any metal ion
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ATP
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47% inhibition at 0.4 mM
Borate
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slight inhibition
Ca2+
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30% inhibition at 0.4 mM
K+
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72% inhibition at 200 mM
Mg2+
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60% inhibition at 8 mM
N-ethylmaleimide
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70% inhibition at 4 mM
Na+
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95% inhibition at 200 mM
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Triton X-100
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stimulation at 1%
additional information
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reducing agents e.g. mercaptoethanol, required for activity, 4fold stimulation
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additional information
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different fractions after ammonium sulfate precipitation
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6.8 - 9
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about 50% of activity maximum at pH 6.8 and 9.0
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brenda
rabbit
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brenda
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brenda
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brenda
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40
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10 min, 75% loss of activity
50
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10 min, complete loss of activity
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2-mercaptoethanol or glycerol does not stabilize
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2°C, 4 days, 75% loss of activity, no stabilization by mercaptoethanol or glycerol
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Raghavan, M.; Lindberg, U.; Schutt, C.
The use of alternative substrates in the characterization of actin-methylating and carnosine-methylating enzymes
Eur. J. Biochem.
210
311-318
1992
Oryctolagus cuniculus
brenda
Vijayasarathy, C.; Rao, B.S.N.
Partial purification and characterisation of S-adenosylmethionine:protein-histidine N-methyltransferase from rabbit skeletal muscle
Biochim. Biophys. Acta
923
156-165
1987
Oryctolagus cuniculus
brenda
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