Information on EC 2.1.1.85 - protein-histidine N-methyltransferase

for references in articles please use BRENDA:EC2.1.1.85
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The expected taxonomic range for this enzyme is: Oryctolagus cuniculus

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EC NUMBER
COMMENTARY hide
2.1.1.85
-
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RECOMMENDED NAME
GeneOntology No.
protein-histidine N-methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + protein L-histidine = S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
show the reaction diagram
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:protein-L-histidine N-tele-methyltransferase
Highly specific for histidine residues, for example, in actin.
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CAS REGISTRY NUMBER
COMMENTARY hide
108022-17-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + protein L-histidine
S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
show the reaction diagram
S-adenosyl-L-methionine + synthetic peptide corresponding to residue 69-77 of actin
S-adenosyl-L-homocysteine + peptide Ntau-methyl-L-histidine
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + protein L-histidine
S-adenosyl-L-homocysteine + protein Ntau-methyl-L-histidine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + synthetic peptide corresponding to residue 69-77 of actin
S-adenosyl-L-homocysteine + peptide Ntau-methyl-L-histidine
show the reaction diagram
-
-
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no requirement for any metal ion
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
-
47% inhibition at 0.4 mM
Borate
-
slight inhibition
Ca2+
-
30% inhibition at 0.4 mM
EDTA
-
slight inhibition
K+
-
72% inhibition at 200 mM
Mg2+
-
60% inhibition at 8 mM
N-ethylmaleimide
-
70% inhibition at 4 mM
Na+
-
95% inhibition at 200 mM
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
-
stimulation at 1%
additional information
-
reducing agents e.g. mercaptoethanol, required for activity, 4fold stimulation
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
different fractions after ammonium sulfate precipitation
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8 - 9
-
about 50% of activity maximum at pH 6.8 and 9.0
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70000
-
gel filtration
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
10 min, 75% loss of activity
50
-
10 min, complete loss of activity
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-mercaptoethanol or glycerol does not stabilize
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
2°C, 4 days, 75% loss of activity, no stabilization by mercaptoethanol or glycerol
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 2.1.1.85)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)