Crystallization (Comment) | Organism |
---|---|
mutant Y200L, with bound S-adenosyl-L-methionine and 3,5-dinitrocatechol, to 1.6 A resolution | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
K144A | mutation of the proposed catalytic Lys residue, does not abolish COMT activity | Rattus norvegicus |
K144A/V173Y | shift towards para-selectivity | Rattus norvegicus |
W38D | mutation of hydrophobic pocket, approximately +93% regioisomeric excess, and around 50% conversion of substrate 3,4-dihydroxybenzaldehyde | Rattus norvegicus |
W38K | mutant favors para- over meta-methylation of dihydroxy substrates | Rattus norvegicus |
W38R | mutant favors para- over meta-methylation of dihydroxy substrates | Rattus norvegicus |
Y200L | mutant favors meta- over para-methylation of dihydroxy substrates, and exhibits the greatest meta-selectivity whilst retaining relative activity similar to that of the wild-type | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P22734 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + (3,4-dihydroxyphenyl)acetic acid | - |
Rattus norvegicus | S-adenosyl-L-homocysteine + (4-hydroxy-3-methoxyphenyl)acetic acid + (3-hydroxy-4-methoxyphenyl)acetic acid | - |
? | |
S-adenosyl-L-methionine + 3,4-dihydroxybenzaldehyde | - |
Rattus norvegicus | S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzaldehyde + 3-hydroxy-4-methoxybenzaldehyde | - |
? | |
S-adenosyl-L-methionine + 3,4-dihydroxybenzoic acid | - |
Rattus norvegicus | S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzoic acid + 3-hydroxy-4-methoxybenzoic acid | - |
? | |
S-adenosyl-L-methionine + 4-nitrobenzene-1,2-diol | - |
Rattus norvegicus | S-adenosyl-L-homocysteine + 2-methoxy-4-nitrophenol + 2-methoxy-5-nitrophenol | - |
? | |
S-adenosyl-L-methionine + a catechol | - |
Rattus norvegicus | S-adenosyl-L-homocysteine + a guaiacol | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
wild-type is independent of pH | Rattus norvegicus |