Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.46 extracted from

  • Li, J.; Li, C.; Gou, J.; Wang, X.; Fan, R.; Zhang, Y.
    An alternative pathway for formononetin biosynthesis in Pueraria lobata (2016), Front. Plant Sci., 7, 861 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.46

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pueraria montana var. lobata

Organism

Organism UniProt Comment Textmining
Pueraria montana var. lobata A0A1L1Z441
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Pueraria montana var. lobata

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Pueraria montana var. lobata
-
root highest expression Pueraria montana var. lobata
-
stem
-
 Pueraria montana var. lobata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is not able to methylate 2,7,4'-trihydroxyisoflavanone, biochanin A, formononetin, apigenin, luteolin, liquirtitigenin, kaempferol, quercetin, isoliquiritigenin, and caffeic acid Pueraria montana var. lobata ?
-
-
S-adenosyl-L-methionine + daidzein 48.8% activity compared to genistein Pueraria montana var. lobata S-adenosyl-L-homocysteine + formononetin
-
 ?
S-adenosyl-L-methionine + genistein 100% activity Pueraria montana var. lobata S-adenosyl-L-homocysteine + biochanin A
-
 ?
S-adenosyl-L-methionine + isoformononetin 5.2% activity compared to genistein Pueraria montana var. lobata S-adenosyl-L-homocysteine + 4',7-dimethoxyisoflavone
-
 ?
S-adenosyl-L-methionine + prunetin 6.2% activity compared to genistein Pueraria montana var. lobata S-adenosyl-L-homocysteine + 4',7-dimethoxy-5-hydroxyisoflavone
-
 ?

Synonyms

Synonyms Comment Organism
I4'OMT
-
 Pueraria montana var. lobata
isoflavone-specific 4'-O-methyltransferase
-
 Pueraria montana var. lobata
OMT9
-
 Pueraria montana var. lobata

Expression

Organism Comment Expression
Pueraria montana var. lobata enzyme expression is increased about 3fold in the presence of methyl jasmonate up