BRENDA - Enzyme Database
show all sequences of 2.1.1.362

Purification and functional characterization of SET8, a nucleosomal histone H4-lysine 20-specific methyltransferase

Fang, J.; Feng, Q.; Ketel, C.S.; Wang, H.; Cao, R.; Xia, L.; Erdjument-Bromage, H.; Tempst, P.; Simon, J.A.; Zhang, Y.; Curr. Biol. 12, 1086-1099 (2002)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
glutathione-S-transferase-fusion protein, SET gene
Drosophila elegans
glutathione-S-transferase-fusion protein, SET gene
Homo sapiens
Engineering
Protein Variants
Commentary
Organism
H299A
overlap PCR, destroyed SET gene, no activity remaining
Drosophila elegans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
nucleus
-
Homo sapiens
5634
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
SDS-PAGE
Drosophila elegans
45000
-
SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Drosophila melanogaster
-
?
-
-
-
additional information
Homo sapiens
histone methylation has significant effects on heterochromatin formation and transcriptional regulation
?
-
-
-
additional information
Drosophila elegans
histone methylation has significant effects on heterochromatin formation and transcriptional regulation
?
-
-
-
S-adenosyl-L-methionine + histone L-lysine
Drosophila melanogaster
-
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
Drosophila melanogaster
?
S-adenosyl-L-methionine + histone L-lysine
Homo sapiens
Lys20 of histone 4, SET8
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
Homo sapiens
?
S-adenosyl-L-methionine + histone L-lysine
Drosophila elegans
Lys20 of histone 4, SET8
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
Drosophila elegans
?
Organism
Organism
UniProt
Commentary
Textmining
Drosophila elegans
-
-
-
Drosophila melanogaster
-
SET8 sequence
-
Homo sapiens
Q9NQR1
HeLa cells; SET8 sequence
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Homo sapiens
Reaction
Reaction
Commentary
Organism
Reaction ID
S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
amino acid sequence comparison; enzyme is encoded on genes containing the SET-domain sequence, substrate specificity is connected to SET sequence
Drosophila elegans
S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
enzyme is encoded on genes containing the SET-domain sequence, substrate specificity is connected to SET sequence
Drosophila melanogaster
S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
amino acid sequence comparison; enzyme is encoded on genes containing the SET-domain sequence, substrate specificity is connected to SET sequence
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
-
485352
Drosophila melanogaster
?
-
-
-
-
additional information
histone methylation has significant effects on heterochromatin formation and transcriptional regulation
485352
Homo sapiens
?
-
-
-
-
additional information
histone methylation has significant effects on heterochromatin formation and transcriptional regulation
485352
Drosophila elegans
?
-
-
-
-
S-adenosyl-L-methionine + histone L-lysine
-
485352
Drosophila melanogaster
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
485352
Drosophila melanogaster
?
S-adenosyl-L-methionine + histone L-lysine
-
485352
Drosophila elegans
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
485352
Drosophila elegans
?
S-adenosyl-L-methionine + histone L-lysine
high substrate and methylation site specifity
485352
Drosophila melanogaster
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
485352
Drosophila melanogaster
?
S-adenosyl-L-methionine + histone L-lysine
high substrate and methylation site specifity
485352
Homo sapiens
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
485352
Homo sapiens
?
S-adenosyl-L-methionine + histone L-lysine
Lys20 of histone 4
485352
Drosophila melanogaster
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
485352
Drosophila melanogaster
?
S-adenosyl-L-methionine + histone L-lysine
Lys20 of histone 4
485352
Homo sapiens
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
485352
Homo sapiens
?
S-adenosyl-L-methionine + histone L-lysine
Lys20 of histone 4, SET8
485352
Homo sapiens
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
485352
Homo sapiens
?
S-adenosyl-L-methionine + histone L-lysine
Lys20 of histone 4, SET8
485352
Drosophila elegans
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
485352
Drosophila elegans
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 45000, SDS-PAGE
Drosophila elegans
monomer
1 * 45000, SDS-PAGE
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
glutathione-S-transferase-fusion protein, SET gene
Drosophila elegans
glutathione-S-transferase-fusion protein, SET gene
Homo sapiens
Engineering (protein specific)
Protein Variants
Commentary
Organism
H299A
overlap PCR, destroyed SET gene, no activity remaining
Drosophila elegans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
nucleus
-
Homo sapiens
5634
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
SDS-PAGE
Drosophila elegans
45000
-
SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Drosophila melanogaster
-
?
-
-
-
additional information
Homo sapiens
histone methylation has significant effects on heterochromatin formation and transcriptional regulation
?
-
-
-
additional information
Drosophila elegans
histone methylation has significant effects on heterochromatin formation and transcriptional regulation
?
-
-
-
S-adenosyl-L-methionine + histone L-lysine
Drosophila melanogaster
-
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
Drosophila melanogaster
?
S-adenosyl-L-methionine + histone L-lysine
Homo sapiens
Lys20 of histone 4, SET8
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
Homo sapiens
?
S-adenosyl-L-methionine + histone L-lysine
Drosophila elegans
Lys20 of histone 4, SET8
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
Drosophila elegans
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
-
485352
Drosophila melanogaster
?
-
-
-
-
additional information
histone methylation has significant effects on heterochromatin formation and transcriptional regulation
485352
Homo sapiens
?
-
-
-
-
additional information
histone methylation has significant effects on heterochromatin formation and transcriptional regulation
485352
Drosophila elegans
?
-
-
-
-
S-adenosyl-L-methionine + histone L-lysine
-
485352
Drosophila melanogaster
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
485352
Drosophila melanogaster
?
S-adenosyl-L-methionine + histone L-lysine
-
485352
Drosophila elegans
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
485352
Drosophila elegans
?
S-adenosyl-L-methionine + histone L-lysine
high substrate and methylation site specifity
485352
Drosophila melanogaster
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
485352
Drosophila melanogaster
?
S-adenosyl-L-methionine + histone L-lysine
high substrate and methylation site specifity
485352
Homo sapiens
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
485352
Homo sapiens
?
S-adenosyl-L-methionine + histone L-lysine
Lys20 of histone 4
485352
Drosophila melanogaster
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
485352
Drosophila melanogaster
?
S-adenosyl-L-methionine + histone L-lysine
Lys20 of histone 4
485352
Homo sapiens
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
485352
Homo sapiens
?
S-adenosyl-L-methionine + histone L-lysine
Lys20 of histone 4, SET8
485352
Homo sapiens
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
485352
Homo sapiens
?
S-adenosyl-L-methionine + histone L-lysine
Lys20 of histone 4, SET8
485352
Drosophila elegans
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
485352
Drosophila elegans
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 45000, SDS-PAGE
Drosophila elegans
monomer
1 * 45000, SDS-PAGE
Homo sapiens
Other publictions for EC 2.1.1.362
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
702886
Souza
The histone methyltransferase ...
Homo sapiens
BMC Cell Biol.
10
41
2009
-
-
1
-
-
-
-
-
1
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
703868
Sakaguchi
Functional characterization of ...
Drosophila melanogaster
Genetics
179
317-322
2008
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
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-
-
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-
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1
-
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1
-
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1
-
-
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-
-
-
-
-
-
-
1
1
-
-
-
485352
Fang
Purification and functional ch ...
Drosophila elegans, Drosophila melanogaster, Homo sapiens
Curr. Biol.
12
1086-1099
2002
-
-
2
-
1
-
-
-
1
-
2
6
-
3
-
-
1
3
-
-
-
-
11
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
2
6
-
-
-
1
-
-
-
-
11
2
-
-
-
-
-
-
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-
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