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Literature summary for 2.1.1.362 extracted from

  • Fang, J.; Feng, Q.; Ketel, C.S.; Wang, H.; Cao, R.; Xia, L.; Erdjument-Bromage, H.; Tempst, P.; Simon, J.A.; Zhang, Y.
    Purification and functional characterization of SET8, a nucleosomal histone H4-lysine 20-specific methyltransferase (2002), Curr. Biol., 12, 1086-1099.
    View publication on PubMed
Search for more literature for 2.1.1.362

Cloned(Commentary)

Cloned (Comment) Organism
glutathione-S-transferase-fusion protein, SET gene Homo sapiens
glutathione-S-transferase-fusion protein, SET gene Drosophila elegans

Protein Variants

Protein Variants Comment Organism
H299A overlap PCR, destroyed SET gene, no activity remaining Drosophila elegans

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Homo sapiens 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 SDS-PAGE Homo sapiens
45000
-
 SDS-PAGE Drosophila elegans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Drosophila melanogaster
-
 ?
-
 ?
additional information Homo sapiens histone methylation has significant effects on heterochromatin formation and transcriptional regulation ?
-
 ?
additional information Drosophila elegans histone methylation has significant effects on heterochromatin formation and transcriptional regulation ?
-
 ?
S-adenosyl-L-methionine + histone L-lysine Drosophila melanogaster
-
 S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?
S-adenosyl-L-methionine + histone L-lysine Homo sapiens Lys20 of histone 4, SET8 S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?
S-adenosyl-L-methionine + histone L-lysine Drosophila elegans Lys20 of histone 4, SET8 S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila elegans
-
-
-
Drosophila melanogaster
-
 SET8 sequence
-
Homo sapiens Q9NQR1 HeLa cells
-
Homo sapiens Q9NQR1 SET8 sequence
-

Purification (Commentary)

Purification (Comment) Organism
partial Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine amino acid sequence comparison Homo sapiens
a
S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine amino acid sequence comparison Drosophila elegans
a
S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine enzyme is encoded on genes containing the SET-domain sequence, substrate specificity is connected to SET sequence Drosophila melanogaster
a
S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine enzyme is encoded on genes containing the SET-domain sequence, substrate specificity is connected to SET sequence Homo sapiens
a
S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine enzyme is encoded on genes containing the SET-domain sequence, substrate specificity is connected to SET sequence Drosophila elegans
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information
-
 Drosophila melanogaster ?
-
 ?
additional information histone methylation has significant effects on heterochromatin formation and transcriptional regulation Homo sapiens ?
-
 ?
additional information histone methylation has significant effects on heterochromatin formation and transcriptional regulation Drosophila elegans ?
-
 ?
S-adenosyl-L-methionine + histone L-lysine
-
 Drosophila melanogaster S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?
S-adenosyl-L-methionine + histone L-lysine
-
 Drosophila elegans S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?
S-adenosyl-L-methionine + histone L-lysine high substrate and methylation site specificity Drosophila melanogaster S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?
S-adenosyl-L-methionine + histone L-lysine high substrate and methylation site specificity Homo sapiens S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?
S-adenosyl-L-methionine + histone L-lysine Lys20 of histone 4 Drosophila melanogaster S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?
S-adenosyl-L-methionine + histone L-lysine Lys20 of histone 4 Homo sapiens S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?
S-adenosyl-L-methionine + histone L-lysine Lys20 of histone 4, SET8 Homo sapiens S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?
S-adenosyl-L-methionine + histone L-lysine Lys20 of histone 4, SET8 Drosophila elegans S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Homo sapiens
monomer 1 * 45000, SDS-PAGE Drosophila elegans