Literature summary for 2.1.1.361 extracted from

Tardat, M.; Brustel, J.; Kirsh, O.; Lefevbre, C.; Callanan, M.; Sardet, C.; Julien, E.
The histone H4 Lys 20 methyltransferase PR-Set7 regulates replication origins in mammalian cells (2010), Nat. Cell Biol., 12, 1086-1093 .

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Protein Variants

Protein Variants	Comment	Organism
F184A/Y185A	the mutations prevent enzyme degradation during S phase	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + a [histone H4]-L-lysine20	Homo sapiens	-	S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9NQR1	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
U2-OS cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + a [histone H4]-L-lysine20	-	Homo sapiens	S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20	-	?

Synonyms

Synonyms	Comment	Organism
histone H4 Lys 20 methyltransferase	-	Homo sapiens
KMT5a	-	Homo sapiens
Pr-Set7	-	Homo sapiens
SET8	-	Homo sapiens

General Information

General Information	Comment	Organism
malfunction	expression of an enzyme mutant insensitive to degradation during S phase causes the maintenance of monomethylated histone H4 lysine 20 and repeated DNA replication at origins	Homo sapiens
physiological function	the enzyme plays a critical role in chromatin events that regulate replication origins. The enzyme undergoes a proliferating cell nuclear antigen- and Cul4-Ddb1 E3-ligase driven degradation during S phase that contributes to the disappearance of monomethylated histone H4 lysine 20 at origins and the inhibition of replication licensing	Homo sapiens

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Release 2023.1 (January 2023)