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Literature summary for 2.1.1.355 extracted from
- Sequence specificity and role of proximal amino acids of the histone H3 tail on catalysis of murine G9A lysine 9 histone H3 methyltransferase (2005), Biochemistry, 44, 12998-13006.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | sequence context of modified residue affects G9a activity and modification in the proximal amino acids influences methylation | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00017 | - |
histone H3(K27A) | recombinant substrate | Mus musculus | |
| 0.00023 | - |
histone H3(K4A) | recombinant substrate | Mus musculus | |
| 0.00026 | - |
histone H3 | recombinant substrate | Mus musculus | |
| 0.0006 | - |
histone K4-trimethylK9 | - |
Mus musculus | |
| 0.0006 | - |
histone H3 (T11phos) | wild-type substrate | Mus musculus | |
| 0.0009 | - |
histone H3 | wild-type substrate | Mus musculus | |
| 0.0011 | - |
histone H3 (1-13) | wild-type substrate | Mus musculus | |
| 0.0015 | - |
histone K4AK9 | - |
Mus musculus | |
| 0.0022 | - |
histone K4-acetylK9 | - |
Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | minimal substrate methylated by G9a contains seven amino acids (TARKSTG) of the histone H3 tail | Mus musculus | ? | - |
? |  |
| S-adenosyl-L-methionine + histone H3 | - |
Mus musculus | ? | - |
? | |
| S-adenosyl-L-methionine + histone H3 (1-13) | - |
Mus musculus | ? | - |
? | |
| S-adenosyl-L-methionine + histone H3 (S10phos) | phosphorylation of the proximal amino acids impairs Lys-9 methylation via impairing enzyme-substrate interaction | Mus musculus | ? | - |
? | |
| S-adenosyl-L-methionine + histone H3 (T11phos) | phosphorylation of the proximal amino acids impairs Lys-9 methylation via impairing enzyme-substrate interaction | Mus musculus | ? | - |
? | |
| S-adenosyl-L-methionine + histone H3(K27A) | mutation in the unstructured amino-terminal tail of histone H3 does not affect the central globular domain, but reduces the turnover numbers of the substrates | Mus musculus | ? | - |
? | |
| S-adenosyl-L-methionine + histone H3(K4A) | mutation in the unstructured amino-terminal tail of histone H3 does not affect the central globular domain, but reduces the turnover numbers of the substrates | Mus musculus | ? | - |
? | |
| S-adenosyl-L-methionine + histone K4-acetylK9 | - |
Mus musculus | ? | - |
? | |
| S-adenosyl-L-methionine + histone K4-trimethylK9 | - |
Mus musculus | ? | - |
? | |
| S-adenosyl-L-methionine + histone K4AK9 | - |
Mus musculus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| G9a | - |
Mus musculus |
| G9a lysine 9 histone H3 methyltransferase | - |
Mus musculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001806 | - |
histone H3 (S10phos) | wild-type substrate | Mus musculus | |
| 0.005 | - |
histone H3 (T11phos) | wild-type substrate | Mus musculus | |
| 0.0089 | - |
histone K4-acetylK9 | - |
Mus musculus | |
| 0.0089 | - |
histone K4-trimethylK9 | - |
Mus musculus | |
| 0.01194 | - |
histone K4AK9 | - |
Mus musculus | |
| 0.0161 | - |
histone H3(K4A) | recombinant substrate | Mus musculus | |
| 0.0161 | - |
histone H3(K27A) | recombinant substrate | Mus musculus | |
| 0.02056 | - |
histone H3 (1-13) | wild-type substrate | Mus musculus | |
| 0.02167 | - |
histone H3 | recombinant substrate | Mus musculus | |
| 0.024 | - |
histone H3 | wild-type substrate | Mus musculus |
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