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Literature summary for 2.1.1.340 extracted from

  • Larsson, K.A.; Saheed, S.A.; Gradin, T.; Delp, G.; Karpinska, B.; Botha, C.E.; Jonsson, L.M.
    Differential regulation of 3-aminomethylindole/N-methyl-3-aminomethylindole N-methyltransferase and gramine in barley by both biotic and abiotic stress conditions (2011), Plant Physiol. Biochem., 49, 96-102.
    View publication on PubMed
Search for more literature for 2.1.1.340

Activating Compound

Activating Compound Comment Organism Structure
additional information gramine levels, but not NMT expression, is increased in barley due to drought and abscisic acid treatment Hordeum vulgare

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Hordeum vulgare subsp. vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.077
-
 3-aminomethylindole pH 7.5, 30°C Hordeum vulgare subsp. vulgare
0.148
-
 S-adenosyl-L-methionine pH 7.5, 30°C Hordeum vulgare subsp. vulgare
0.184
-
 N-methyl-3-aminomethylindole pH 7.5, 30°C Hordeum vulgare subsp. vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, SDS-PAGE Hordeum vulgare subsp. vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
-
-
Hordeum vulgare subsp. vulgare Q96565
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + 3-aminomethylindole
-
 Hordeum vulgare subsp. vulgare 2 S-adenosyl-L-homocysteine + gramine overall reaction, two successive N-methylation reactions ?
2 S-adenosyl-L-methionine + N-methyl-3-aminomethylindole
-
 Hordeum vulgare subsp. vulgare 2 S-adenosyl-L-homocysteine + (1H-indol-3-yl)-N,N-dimethylmethanamine
-
 ?
additional information no substrate: caffeic acid Hordeum vulgare subsp. vulgare ?
-
 ?
S-adenosyl-L-methionine + 3-aminomethylindole
-
 Hordeum vulgare subsp. vulgare S-adenosyl-L-homocysteine + N-methyl-3-aminomethylindole
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Hordeum vulgare subsp. vulgare

Synonyms

Synonyms Comment Organism
NMT
-
 Hordeum vulgare

Expression

Organism Comment Expression
Hordeum vulgare expression is induced by methyl jasmonate. Infestation by Rhopalosiphum padi and Metopolophium dirhodum results in higher expression in the doubled haploid line 5172-28:4. None of the aphide-plant combinations displays increased gramine levels, and the increased abundance of transcript in aphid-infested DH28:4 does not lead to higher amounts of NMT protein or NMT enzyme activity up