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Literature summary for 2.1.1.216 extracted from

  • Arimbasseri, A.; Blewett, N.; Iben, J.; Lamichhane, T.; Cherkasova, V.; Hafner, M.; Maraia, R.
    RNA polymerase III output is functionally linked to tRNA dimethyl-G26 modification (2015), PLoS Genet., 11, e1005671 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.216

Protein Variants

Protein Variants Comment Organism
additional information generation of a trm1DELTA knockout mutant Schizosaccharomyces pombe

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Schizosaccharomyces pombe 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl-L-methionine + guanine26 in tRNA Schizosaccharomyces pombe
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2 S-adenosyl-L-methionine + guanine26 in tRNA Schizosaccharomyces pombe 972
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe Q9P804
-
-
Schizosaccharomyces pombe 972 Q9P804
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Schizosaccharomyces pombe 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Schizosaccharomyces pombe 972 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?

Synonyms

Synonyms Comment Organism
Trm1
-
 Schizosaccharomyces pombe

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Schizosaccharomyces pombe

General Information

General Information Comment Organism
malfunction treatment with rapamycin or overexpression of maf1+ reduces tRNA transcription with increase in the m22G26 content of sup-tRNASerUCA and its specific activity for suppression. Mutations in a catalytic subunit of RNAP III associated with hypomyelinating leukodystrophy (HLD) to show that a general decrease in tRNA transcription by another mechanism also increases m22G26 modification efficiency and reverses antisuppression in Schizosaccharomyces pombe. G26 misincorporations are decreased to below 1% in trm1DELTA mutant cells. Expression of trm1+ from a high copy plasmid in trm1DELTA cells restores the misincorporations to higher levels than in wild-type cells Schizosaccharomyces pombe
metabolism the tRNA modification dimethyl-guanine-26 (m22G26) varies in the efficiency by which it is added to its target tRNAs, in a manner that is dependent on the overall activity rate of RNA polymerase III (RNAP III) that synthesizes the tRNAs. Rapamycin decreases tRNA synthesis in a maf1+-dependent manner in Schizosaccharomyces pombe. Hypomodification of m22G26 is responsible for maf1DELTA antisuppression. RNAP III mutations that decrease tRNA synthesis also increase m22G26 efficiency and tRNA activity Schizosaccharomyces pombe
physiological function after their synthesis by RNAP III, tRNAs are chemically modified by enzymes on multiple of their nucleosides, also involving the enzyme. G26 is modified by N2,N2 dimethylation (m2 2G26) in many tRNAs by the Trm1 methyltransferase. Trm1 is a nuclear enzyme that produces m2 2G26 which likely contributes to proper tRNA folding. N2,N2-dimethyl G26 (m22G26) can enhance tRNA function. Trm1 activity is limiting in the context of increased tRNA production in maf1DELTA cells and its overexpression reverses antisuppression. Of the 36 tRNAs in Schizosaccharomyces pombe that have G26, 27 show significant misincorporation at G26. The extent of G26 misincorporation varies with tRNA identity from 10-80%, G26 misincorporations are due to m22G26 modification. A significant amount of Trm1 substrates are not fully modified in wild-type cells because Trm1 activity is limiting. Hypomodification of m22G26 is responsible for maf1DELTA antisuppression. m22G26 modification debilitates normal base pairing. Efficiency of m22G26 modification responds to growth/nutrient conditions, overview Schizosaccharomyces pombe