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Information on EC 2.1.1.216 - tRNA (guanine26-N2)-dimethyltransferase
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EC Tree
2.1.1.216 tRNA (guanine26-N2)-dimethyltransferaseIUBMB Comments
The enzyme dissociates from its tRNA substrate between the two consecutive methylation reactions. In contrast to EC 2.1.1.215, tRNA (guanine26-N2/guanine27-N2)-dimethyltransferase, this enzyme does not catalyse the methylation of guanine27 in tRNA.
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Word Map
The expected taxonomic range for this enzyme is: Eukaryota, Archaea
Reaction Schemes
2
+
guanine26 in tRNA
=
2
+
N2-dimethylguanine26 in tRNA
Synonyms
trm1p, zc376.5, ta0997, trna (m22g26)dimethyltransferase, trm1 methyltransferase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
EC 2.1.1.32
-
-
formerly, part transferred
-
Ta0997
transfer RNA methyltransferase
Trm1
Trm1p
tRNA(m22G26)dimethyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 S-adenosyl-L-methionine + guanine26 in tRNA = 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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-
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:tRNA (guanine26-N2)-dimethyltransferase
The enzyme dissociates from its tRNA substrate between the two consecutive methylation reactions. In contrast to EC 2.1.1.215, tRNA (guanine26-N2/guanine27-N2)-dimethyltransferase, this enzyme does not catalyse the methylation of guanine27 in tRNA.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2 S-adenosyl-L-methionine + guanine26 in elongator tRNAMet
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in elongator tRNAMet
2 S-adenosyl-L-methionine + guanine26 in initiator tRNAMet
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in initiator tRNAMet
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
2 S-adenosyl-L-methionine + guanine26 in tRNAPhe
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNAPhe
4 S-adenosyl-L-methionine + guanine26 in initiator tRNAMet
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in initiator tRNAMet
m2G26 and m22G26 are formed by Trm1
-
-
?
4 S-adenosyl-L-methionine + guanine26 in prolongator tRNAMet
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in prolongator tRNAMet
m2G26 and m22G26 are formed by Trm1
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNA
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
4 S-adenosyl-L-methionine + guanine26 in tRNALeuCAA
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeuCAA
-
-
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNALeuTAG
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeuTAG
-
-
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNASerUCA
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNASerUCA
-
-
-
-
?
S-adenosyl-L-methionine + 2'-O-methylguanine26 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-dimethylguanine26 in elongator tRNAMet
-
-
-
?
S-adenosyl-L-methionine + 2'-O-methylguanine26 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-dimethylguanine26 in initiator tRNAMet
-
-
-
?
S-adenosyl-L-methionine + guanine26 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylguanine26 in elongator tRNAMet
-
-
-
?
S-adenosyl-L-methionine + guanine26 in elongator tRNAMet
S-adenosyl-L-homocysteine + N2-methylguanine26 in elongator tRNAMet
-
-
-
?
S-adenosyl-L-methionine + guanine26 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylguanine26 in initiator tRNAMet
-
-
-
?
S-adenosyl-L-methionine + guanine26 in initiator tRNAMet
S-adenosyl-L-homocysteine + N2-methylguanine26 in initiator tRNAMet
-
-
-
?
S-adenosyl-L-methionine + guanine26 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
S-adenosyl-L-methionine + guanine26 in tRNAPhe
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNAPhe
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
S-adenosyl-L-methionine + N2-methylguanine26 in tRNAPhe
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNAPhe
2 S-adenosyl-L-methionine + guanine26 in elongator tRNAMet
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in elongator tRNAMet
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in elongator tRNAMet
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in elongator tRNAMet
-
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in initiator tRNAMet
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in initiator tRNAMet
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in initiator tRNAMet
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in initiator tRNAMet
-
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
wild-type enzyme produces 88% N2-dimethylguanine26 tRNA and 12% N2-methylguanine26 in tRNA. The Caenorhabditis elegans tRNA(m22G26)dimethyltransferase efficiently recognized the Escherichia coli tRNA as a substrate in vivo, and that the enzyme is capable of both mono- and dimethylating G26 in this tRNA
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
-
the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
-
the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group. The methylated intermediate, and the enzyme dissociates from its tRNA substrate between the two consecutive methylation reactions
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
-
the elements necessary for a productive interaction between G26 in nuclear coded yeast tRNAs and the yeast G26 modifying enzyme are located within the core of the tRNA
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
wild-type enzyme produces 94% N2-dimethylguanine26 tRNA and 6% N2-methylguanine26 in tRNA
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNAPhe
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNAPhe
tRNAPhe from yeast
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNAPhe
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNAPhe
-
tRNAPhe from yeast, the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNA
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
G26 is modified by N2,N2-dimethylation in many tRNAs by the Trm1 methyltransferase
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNA
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNA
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
G modification at position 26 is a mixture of m2G and m22G generated by Trm1
-
-
?
S-adenosyl-L-methionine + guanine26 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine26 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
-
-
-
?
S-adenosyl-L-methionine + guanine26 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine26 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine26 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
-
-
-
?
S-adenosyl-L-methionine + guanine26 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
the elements necessary for a productive interaction between G26 in nuclear coded yeast tRNAs and the yeast G26 modifying enzyme are located within the core of the tRNA
-
-
?
S-adenosyl-L-methionine + guanine26 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine26 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine26 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine26 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine26 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine26 in tRNAPhe
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNAPhe
-
tRNAPhe from yeast
-
-
?
S-adenosyl-L-methionine + guanine26 in tRNAPhe
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNAPhe
tRNAPhe from yeast
-
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group
-
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group. The methylated intermediate, and the enzyme dissociates from its tRNA substrate between the two consecutive methylation reactions
-
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
the elements necessary for a productive interaction between G26 in nuclear coded yeast tRNAs and the yeast G26 modifying enzyme are located within the core of the tRNA
-
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNAPhe
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNAPhe
tRNAPhe from yeast
-
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNAPhe
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNAPhe
-
tRNAPhe from yeast, the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group
-
-
?
additional information
?
-
-
in yeast tRNAAsp G26 is unmodified. Successive change of the near surroundings of G26 in this tRNA is performed until G26 becomes modified to N2-dimethylguanine26 tRNA by a tRNA(m2(2)G26)methyltransferase in Xenopus laevis oocytes. In this the two D-stem basepairs C1 -G24, G10-C25 are identified immediately preceding G26 as major identity elements for the dimethylating enzyme modifying G26. Increasing the extra loop in tRNAAsp from four to the more usual five bases influence the global structure of the tRNA such that the mJG26 formation is drastically decreased even if the near region of G26 had the two consensus basepairs. Not only are the two consensus base pairs in the D-stem a prerequisite for G26 modification, but also is any part of the tRNA molecule that influence the 3D-structure important for the recognition between nuclear coded tRNAs and the tRNA(mJG26)methyltransferase
-
-
?
additional information
?
-
-
the enzyme Trm1 is single site-specific
-
-
?
additional information
?
-
the enzyme Trm1 is single site-specific
-
-
?
additional information
?
-
Ta0997 gene product is a single site-specific Trm1
-
-
-
additional information
?
-
-
Ta0997 gene product is a single site-specific Trm1
-
-
-
additional information
?
-
Ta0997 gene product is a single site-specific Trm1
-
-
-
additional information
?
-
Ta0997 gene product is a single site-specific Trm1
-
-
-
additional information
?
-
Ta0997 gene product is a single site-specific Trm1
-
-
-
additional information
?
-
Ta0997 gene product is a single site-specific Trm1
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
-
the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
4 S-adenosyl-L-methionine + guanine26 in initiator tRNAMet
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in initiator tRNAMet
m2G26 and m22G26 are formed by Trm1
-
-
?
4 S-adenosyl-L-methionine + guanine26 in prolongator tRNAMet
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in prolongator tRNAMet
m2G26 and m22G26 are formed by Trm1
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNA
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
4 S-adenosyl-L-methionine + guanine26 in tRNALeuCAA
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeuCAA
-
-
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNALeuTAG
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeuTAG
-
-
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNASerUCA
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNASerUCA
-
-
-
-
?
S-adenosyl-L-methionine + 2'-O-methylguanine26 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-dimethylguanine26 in elongator tRNAMet
-
-
-
?
S-adenosyl-L-methionine + 2'-O-methylguanine26 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-dimethylguanine26 in initiator tRNAMet
-
-
-
?
S-adenosyl-L-methionine + guanine26 in elongator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylguanine26 in elongator tRNAMet
-
-
-
?
S-adenosyl-L-methionine + guanine26 in initiator tRNAMet
S-adenosyl-L-homocysteine + 2'-O-methylguanine26 in initiator tRNAMet
-
-
-
?
S-adenosyl-L-methionine + guanine26 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
-
-
?
2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
-
-
?
4 S-adenosyl-L-methionine + guanine26 in tRNA
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
G26 is modified by N2,N2-dimethylation in many tRNAs by the Trm1 methyltransferase
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4 S-adenosyl-L-methionine + guanine26 in tRNA
4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
G modification at position 26 is a mixture of m2G and m22G generated by Trm1
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S-adenosyl-L-methionine + guanine26 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
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?
S-adenosyl-L-methionine + guanine26 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
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?
S-adenosyl-L-methionine + guanine26 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
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?
S-adenosyl-L-methionine + guanine26 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
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?
S-adenosyl-L-methionine + guanine26 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
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?
S-adenosyl-L-methionine + guanine26 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
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?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
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the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group
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S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
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?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
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?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
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?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
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?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
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?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
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the efficiency of N2,N2-dimethyl G26 modification on certain tRNAs is decreased in response to maf1-deletion and is associated with antisuppression
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Rapamycin
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a decrease in tRNA synthesis by treatment with rapamycin leads to increased N2,N2-dimethyl G26 modification
additional information
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RNA polymerase III mutations associated with hypomyelinating leukodystrophy decrease tRNA transcription, increase of N2,N2-dimethyl G26 modification efficiency and reverse antisuppression
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.6
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
additional information
Thermoplasma acidophilum grows optimally at 59°C and pH 1.9
brenda
additional information
-
Thermoplasma acidophilum grows optimally at 59°C and pH 1.9
brenda
additional information
-
Thermoplasma acidophilum grows optimally at 59°C and pH 1.9
-
brenda
additional information
-
Thermoplasma acidophilum grows optimally at 59°C and pH 1.9
-
brenda
additional information
-
Thermoplasma acidophilum grows optimally at 59°C and pH 1.9
-
brenda
additional information
-
Thermoplasma acidophilum grows optimally at 59°C and pH 1.9
-
brenda