BRENDA - Enzyme Database show
show all sequences of 2.1.1.208

The avilamycin resistance determinants AviRa and AviRb methylate 23S rRNA at the guanosine 2535 base and the uridine 2479 ribose

Treede, I.; Jakobsen, L.; Kirpekar, F.; Vester, B.; Weitnauer, G.; Bechthold, A.; Douthwaite, S.; Mol. Microbiol. 49, 309-318 (2003)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + uridine2479 in 23S rRNA
Streptomyces viridochromogenes
AviRb modifies the 2'-O-ribose position of nucleotide uridine2479 within helix 89
S-adenosyl-L-homocysteine + 2'-O-methyluridine2479 in 23S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces viridochromogenes
Q9F5K6
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + uridine2479 in 23S rRNA
AviRb modifies the 2'-O-ribose position of nucleotide uridine2479 within helix 89
709992
Streptomyces viridochromogenes
S-adenosyl-L-homocysteine + 2'-O-methyluridine2479 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + uridine2479 in 23S rRNA
AviRb modifies the 2'-O-ribose position of nucleotide uridine2479 within helix 89. The exact position of the modification is conformed by MALDI mass spectrometry, a single methyl group is added. No methylation activity with 16S rRNA
709992
Streptomyces viridochromogenes
S-adenosyl-L-homocysteine + 2'-O-methyluridine2479 in 23S rRNA
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Streptomyces viridochromogenes
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Streptomyces viridochromogenes
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + uridine2479 in 23S rRNA
Streptomyces viridochromogenes
AviRb modifies the 2'-O-ribose position of nucleotide uridine2479 within helix 89
S-adenosyl-L-homocysteine + 2'-O-methyluridine2479 in 23S rRNA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + uridine2479 in 23S rRNA
AviRb modifies the 2'-O-ribose position of nucleotide uridine2479 within helix 89
709992
Streptomyces viridochromogenes
S-adenosyl-L-homocysteine + 2'-O-methyluridine2479 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + uridine2479 in 23S rRNA
AviRb modifies the 2'-O-ribose position of nucleotide uridine2479 within helix 89. The exact position of the modification is conformed by MALDI mass spectrometry, a single methyl group is added. No methylation activity with 16S rRNA
709992
Streptomyces viridochromogenes
S-adenosyl-L-homocysteine + 2'-O-methyluridine2479 in 23S rRNA
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Streptomyces viridochromogenes
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Streptomyces viridochromogenes
Other publictions for EC 2.1.1.208
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
709512
Mosbacher
Structure and function of the ...
Streptomyces viridochromogenes
J. Mol. Biol.
345
535-545
2005
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1
1
7
1
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3
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3
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1
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709992
Treede
The avilamycin resistance dete ...
Streptomyces viridochromogenes
Mol. Microbiol.
49
309-318
2003
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1
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2
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1
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1
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707163
Weitnauer
An ATP-binding cassette transp ...
Streptomyces viridochromogenes, Streptomyces viridochromogenes Tu57
Antimicrob. Agents Chemother.
45
690-695
2001
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1
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5
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1
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