Streptomyces viridochromogenes produces the antibiotic avilamycin A which binds to the 50S ribosomal subunit to inhibit protein synthesis. To protect itself from the antibiotic, Streptomyces viridochromogenes utilizes two methyltransferases, 23S rRNA (uridine2479-2′-O)-methyltransferase and EC 2.1.1.209 [23S rRNA (guanine2535-N1)-methyltransferase], whose actions confer avilamycin resistance to the RNA.
The expected taxonomic range for this enzyme is: Streptomyces viridochromogenes
Streptomyces viridochromogenes produces the antibiotic avilamycin A which binds to the 50S ribosomal subunit to inhibit protein synthesis. To protect itself from the antibiotic, Streptomyces viridochromogenes utilizes two methyltransferases, 23S rRNA (uridine2479-2'-O)-methyltransferase and EC 2.1.1.209 [23S rRNA (guanine2535-N1)-methyltransferase], whose actions confer avilamycin resistance to the RNA.
Substrates: AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of uridine2479 of the 23S ribosomal RNA in Gram-positive bacteria and thus mediates resistance to the oligosaccharide antibiotic avilamycin Products: -
?
S-adenosyl-L-methionine + uridine2479 in 23S rRNA
S-adenosyl-L-homocysteine + 2'-O-methyluridine2479 in 23S rRNA
Substrates: AviRb modifies the 2'-O-ribose position of nucleotide uridine2479 within helix 89. The exact position of the modification is conformed by MALDI mass spectrometry, a single methyl group is added. No methylation activity with 16S rRNA Products: -
Substrates: AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of uridine2479 of the 23S ribosomal RNA in Gram-positive bacteria and thus mediates resistance to the oligosaccharide antibiotic avilamycin Products: -
?
S-adenosyl-L-methionine + uridine2479 in 23S rRNA
S-adenosyl-L-homocysteine + 2'-O-methyluridine2479 in 23S rRNA
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapour-diffusion method at 20°C. Crystals obtained belong to space group P4(1)2(1)2 and contained two subunits of AviRb per crystallographic asymmetric unit
Weitnauer, G.; Gaisser, S.; Trefzer, A.; Stockert, S.; Westrich, L.; Quiros, L.M.; Mendez, C.; Salas, J.A.; Bechthold, A.
An ATP-binding cassette transporter and two rRNA methyltransferases are involved in resistance to avilamycin in the producer organism Streptomyces viridochromogenes T57
show
top
