BRENDA - Enzyme Database
show all sequences of 2.1.1.14

Cobalamin-independent methionine synthase (MetE): a face-to-face double barrel that evolved by gene duplication

Pejchal, R.; Ludwig, M.L.; PLoS Biol. 3, e31 (2005)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3)Star cells
Thermotoga maritima
Crystallization (Commentary)
Crystallization (Commentary)
Organism
vapor batch (microbatch) method using 25% poly(ethylene glycol) 4000, 0.2 M ammonium sulfate, and 0.1 M sodium acetate (pH 4.6)
Thermotoga maritima
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
requires zinc for activity
Arabidopsis thaliana
Zn2+
requires zinc for activity
Escherichia coli
Zn2+
requires zinc for activity
Saccharomyces cerevisiae
Zn2+
requires zinc for activity
Thermotoga maritima
Organism
Organism
UniProt
Commentary
Textmining
Arabidopsis thaliana
O50008
-
-
Escherichia coli
P25665
-
-
Saccharomyces cerevisiae
P05694
-
-
Thermotoga maritima
Q9X112
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Zn(II)-NTA column chromatography
Thermotoga maritima
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
676741
Thermotoga maritima
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
676741
Arabidopsis thaliana
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
676741
Escherichia coli
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
676741
Saccharomyces cerevisiae
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase
-
Arabidopsis thaliana
5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase
-
Escherichia coli
5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase
-
Saccharomyces cerevisiae
5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase
-
Thermotoga maritima
cobalamin-independent methionine synthase
-
Arabidopsis thaliana
cobalamin-independent methionine synthase
-
Escherichia coli
cobalamin-independent methionine synthase
-
Saccharomyces cerevisiae
cobalamin-independent methionine synthase
-
Thermotoga maritima
MetE
-
Arabidopsis thaliana
MetE
-
Saccharomyces cerevisiae
MetE
-
Thermotoga maritima
MetE
-
Escherichia coli
TM1286
-
Arabidopsis thaliana
TM1286
-
Escherichia coli
TM1286
-
Saccharomyces cerevisiae
TM1286
-
Thermotoga maritima
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
vitamin-B12 independent enzyme
Saccharomyces cerevisiae
additional information
vitamin-B12 independent enzyme
Arabidopsis thaliana
additional information
vitamin B12-independent enzyme
Escherichia coli
additional information
vitamin B12-independent enzyme
Thermotoga maritima
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3)Star cells
Thermotoga maritima
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
vitamin-B12 independent enzyme
Arabidopsis thaliana
additional information
vitamin B12-independent enzyme
Escherichia coli
additional information
vitamin-B12 independent enzyme
Saccharomyces cerevisiae
additional information
vitamin B12-independent enzyme
Thermotoga maritima
Crystallization (Commentary) (protein specific)
Crystallization
Organism
vapor batch (microbatch) method using 25% poly(ethylene glycol) 4000, 0.2 M ammonium sulfate, and 0.1 M sodium acetate (pH 4.6)
Thermotoga maritima
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
requires zinc for activity
Arabidopsis thaliana
Zn2+
requires zinc for activity
Escherichia coli
Zn2+
requires zinc for activity
Saccharomyces cerevisiae
Zn2+
requires zinc for activity
Thermotoga maritima
Purification (Commentary) (protein specific)
Commentary
Organism
Zn(II)-NTA column chromatography
Thermotoga maritima
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
676741
Thermotoga maritima
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
676741
Arabidopsis thaliana
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
676741
Escherichia coli
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
676741
Saccharomyces cerevisiae
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
Other publictions for EC 2.1.1.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733198
Yang
Proteomic insights into metabo ...
Saccharopolyspora spinosa
Appl. Microbiol. Biotechnol.
99
8629-8641
2015
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1
1
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734490
Ubhi
The cobalamin-independent meth ...
Candida albicans, Candida albicans ATCC MYA-2876
J. Mol. Biol.
427
901-909
2015
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1
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1
4
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2
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2
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1
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4
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2
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2
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735002
Saint-Macary
Methionine biosynthesis is ess ...
Pyricularia oryzae
PLoS ONE
10
e0111108
2015
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7
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1
1
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733170
Mordukhova
Evolved cobalamin-independent ...
Escherichia coli
Appl. Environ. Microbiol.
79
7905-7915
2013
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1
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2
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2
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3
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720398
Fujiwara
Mechanism of methionine syntha ...
Escherichia coli
Microbiology
158
917-924
2012
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1
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1
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720259
Fu
Crystal structures of cobalami ...
Streptococcus mutans
J. Mol. Biol.
412
688-697
2011
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1
1
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1
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4
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1
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1
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1
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1
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1
1
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704294
Hondorp
Oxidation of cysteine 645 of c ...
Escherichia coli
J. Bacteriol.
191
3407-3410
2009
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1
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1
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706748
Gonzalez-Verdejo
-
Isolation and expression analy ...
Phelipanche ramosa
Sci. Hortic.
116
337-341
2008
-
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3
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1
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1
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671122
Fu
Preparation, crystallization a ...
Streptococcus mutans
Acta Crystallogr. Sect. F
62
984-985
2006
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1
1
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1
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1
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1
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1
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1
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-
672156
Taurog
Synergistic, random sequential ...
Escherichia coli, Escherichia coli pJG816
Biochemistry
45
5083-5091
2006
-
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1
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2
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1
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2
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2
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1
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1
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1
1
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1
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2
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1
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672157
Taurog
Activation of methyltetrahydro ...
Escherichia coli
Biochemistry
45
5092-5102
2006
-
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1
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1
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657437
Huang
Cloning and identification of ...
Komagataella pastoris
Acta Biochim. Biophys. Sin.
37
371-378
2005
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1
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1
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3
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660333
Pejchal
-
Cobalamin-independent methioni ...
Thermotoga maritima
PLoS Biol.
3
254-265
2005
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1
1
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1
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1
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671587
Suliman
Purification and properties of ...
Candida albicans, Saccharomyces cerevisiae
Arch. Biochem. Biophys.
441
56-63
2005
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2
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8
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4
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2
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2
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10
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4
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8
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2
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2
2
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8
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2
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10
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8
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676741
Pejchal
Cobalamin-independent methioni ...
Arabidopsis thaliana, Escherichia coli, Saccharomyces cerevisiae, Thermotoga maritima
PLoS Biol.
3
e31
2005
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1
1
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4
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5
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1
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4
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16
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1
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659400
Ferrer
Crystal structures of cobalami ...
Arabidopsis thaliana
J. Biol. Chem.
279
44235-44238
2004
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660332
Hondorp
Oxidative stress inactivates c ...
Escherichia coli
PLoS Biol.
2
1738-1753
2004
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441333
Zeh
Cloning and characterization o ...
Solanum tuberosum
Plant Mol. Biol.
48
255-265
2002
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441218
Peariso
characterization of the zinc s ...
Escherichia coli
Biochemistry
40
987-993
2001
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1
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441217
Zhou
L-Selenohomocysteine: One-step ...
Escherichia coli
Bioorg. Med. Chem. Lett.
10
2471-2475
2000
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2
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441337
Eckermann
Plant methionine synthase: new ...
Catharanthus roseus
Biol. Chem.
381
695-703
2000
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2
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3
1
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1
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4
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1
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1
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441332
Gonzalez
Cobalamin-independent methioni ...
Escherichia coli
Biochemistry
35
12228-12234
1996
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441331
Eichel
Vitamin-B12-independent methio ...
Catharanthus roseus
Eur. J. Biochem.
230
1053-1058
1995
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1
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1
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1
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6
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1
1
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1
1
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441175
Gonzalez
Comparison of cobalamin-indepe ...
Escherichia coli
Biochemistry
31
6045-6056
1992
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441322
Madison
-
Sulphur metabolism. F. Enzymes ...
Daucus carota, Hordeum vulgare, Phaseolus sp., Pisum sativum, Spinacia oleracea
Methods Plant Biochem.
3
361-369
1990
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5
6
5
10
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5
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1
5
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10
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23
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5
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6
5
10
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5
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1
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10
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23
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3
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441329
Chu
Cloning and expression of the ...
Escherichia coli
Arch. Biochem. Biophys.
239
467-474
1985
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1
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1
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441330
Burton
Regulation of methionine biosy ...
Neurospora crassa
Arch. Biochem. Biophys.
168
219-229
1975
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441326
Taylor
-
N5-Methyltetrahydrofolate-homo ...
Auxenochlorella pyrenoidosa, Escherichia coli, Klebsiella aerogenes, Neurospora crassa, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
9
121-165
1973
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6
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16
3
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6
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1
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3
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6
1
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2
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441327
Salem
Folic acid and the methylation ...
Bacillus subtilis
Biochem. J.
126
993-1004
1972
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441328
Burton
-
Methionine synthesis (Yeast) ...
Saccharomyces cerevisiae
Methods Enzymol.
17B
388-392
1971
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1
1
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441323
Whitefield
Purification and properties of ...
Escherichia coli
J. Biol. Chem.
245
390-401
1970
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5
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2
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5
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2
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1
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1
1
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441334
Burton
The formation of methionine fr ...
Phaseolus vulgaris
Biochem. Biophys. Res. Commun.
36
228-234
1969
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441172
Guest
Transfer of the methyl group f ...
Escherichia coli
Biochem. J.
92
497-504
1964
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